{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T04:13:22Z","timestamp":1775708002195,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T00:00:00Z","timestamp":1511827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EUROTRANS-BIO Programme","award":["E01\/0785\/02\/X15"],"award-info":[{"award-number":["E01\/0785\/02\/X15"]}]},{"name":"MIUR-PRIN Programme","award":["2015RNWJAM"],"award-info":[{"award-number":["2015RNWJAM"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A cost-effective immunosensor for the detection and isolation of dental pulp stem cells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specific monoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCM surface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin\u2013avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with a mixed cell population, was analyzed; the results indicated that the developed QCM immunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell from samples with high complexity, being simple, selective, and providing for more convenient and time-saving operations.<\/jats:p>","DOI":"10.3390\/s17122747","type":"journal-article","created":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T11:23:50Z","timestamp":1511868230000},"page":"2747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7829-1907","authenticated-orcid":false,"given":"Ornella","family":"Maglio","sequence":"first","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"},{"name":"Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy"}]},{"given":"Salvatore","family":"Costanzo","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2219-6353","authenticated-orcid":false,"given":"Rosaria","family":"Cercola","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]},{"given":"Gerardo","family":"Zambrano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]},{"given":"Marco","family":"Mauro","sequence":"additional","affiliation":[{"name":"Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy"}]},{"given":"Raffaele","family":"Battaglia","sequence":"additional","affiliation":[{"name":"Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy"}]},{"given":"Gianluca","family":"Ferrini","sequence":"additional","affiliation":[{"name":"Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy"}]},{"given":"Flavia","family":"Nastri","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]},{"given":"Vincenzo","family":"Pavone","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2013-3009","authenticated-orcid":false,"given":"Angela","family":"Lombardi","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Napoli \u201cFederico II\u201d Via Cintia, 80126 Napoli, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2017,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1039\/b719389k","article-title":"Quartz crystal microbalance immunoassay for carcinoma antigen 125 based on gold nanowire-functionalized biomimetic interface","volume":"133","author":"Tang","year":"2008","journal-title":"Analyst"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0003-2670(94)00579-B","article-title":"Highly sensitive quartz crystal immunosensors for multisample detection of herbicides","volume":"304","author":"Yokoyama","year":"1995","journal-title":"Anal. Chim. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.aca.2009.12.004","article-title":"A study of glycoprotein\u2013lectin interactions using quartz crystal microbalance","volume":"668","author":"Yakovleva","year":"2010","journal-title":"Anal. Chim. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.aca.2010.11.054","article-title":"An enzyme-free quartz crystal microbalance biosensor for sensitive glucose detection in biological fluids based on glucose\/dextran displacement approach","volume":"686","author":"Tang","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8540","DOI":"10.3390\/s150408540","article-title":"A Label-Free Immunosensor for Ultrasensitive Detection of Ketamine Based on Quartz Crystal Microbalance","volume":"15","author":"Yang","year":"2015","journal-title":"Sensors"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.talanta.2017.05.075","article-title":"Flexible immunosensor for the detection of salivary \u03b1-amylase in body fluids","volume":"174","author":"Funaria","year":"2017","journal-title":"Talanta"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez, R., Garc\u00eda, P., Garc\u00eda, M., Garc\u00eda, J.V., Jim\u00e9nez, Y., and Arnau, A. (2017). Design and Validation of a 150 MHz HFFQCM Sensor for Bio-Sensing Applications. Sensors, 17.","DOI":"10.3390\/s17092057"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Della Ventura, B., Iannaccone, M., Funaria, R., Pica Ciamarra, M., Altucci, C., Capparelli, R., Roperto, S., and Velotta, R. (2017). Effective antibodies immobilization and functionalized nanoparticles in a quartz-crystal microbalance-based immunosensor for the detection of parathion. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0171754"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.talanta.2017.01.054","article-title":"Development of a QCM-D biosensor for Ochratoxin A detection in red wine","volume":"166","author":"Karczmarczyka","year":"2017","journal-title":"Talanta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1039\/C6EM00628K","article-title":"Probing the interactions of organic molecules, nanomaterials, and microbes with solid surfaces using quartz crystal microbalances: Methodology, advantages, and limitations","volume":"19","author":"Rixiang","year":"2017","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Huang, X., Bai, Q., Hu, J., and Hou, D. (2017). A Practical Model of Quartz Crystal Microbalance in Actual Applications. Sensors, 17.","DOI":"10.3390\/s17081785"},{"key":"ref_12","first-page":"151","article-title":"Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions","volume":"19","author":"Dixon","year":"2008","journal-title":"J. Biomol. Tech."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/BF01337937","article-title":"Verwendung Von Schwingquarzen Zur Wagung Dunner Schichten Und Zur Mikrowagung","volume":"155","author":"Sauerbrey","year":"1959","journal-title":"Z. Phys."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1021\/bm020116i","article-title":"Quartz Crystal Microbalance: A Useful Tool for Studying Thin Polymer Films and Complex Biomolecular Systems at the Solution-Surface Interface","volume":"4","author":"Marx","year":"2003","journal-title":"Biomacromolecules"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12524","DOI":"10.1021\/acs.analchem.6b04303","article-title":"Integration of Quartz Crystal Microbalance-Dissipation and Reflection-Mode Localized Surface Plasmon Resonance Sensors for Biomacromolecular Interaction Analysis","volume":"88","author":"Ferhan","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Dirri, F., Palomba, E., Longobardo, A., Biondi, D., Boccaccini, A., Bortolino, S., Scaccabarozzi, D., and Zampetti, E. (2017, January 21\u201323). QCM-based sensor for volatile organic compounds characterization. Proceedings of the 2017 IEEE International Workshop on Metrology for AeroSpace (MetroAeroSpace), Padua, Italy.","DOI":"10.1109\/MetroAeroSpace.2017.7999547"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1039\/B314345G","article-title":"Calix[4]arene-functionalized poly-cyclopenta[2,1-b;3,4-b\u2032]bithiophenes with good recognition ability and selectivity for small organic molecules for application in QCM-based sensors","volume":"14","author":"Rizzo","year":"2004","journal-title":"J. Mater. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1002\/jmr.1117","article-title":"A survey of the 2006\u20132009 quartz crystal microbalance biosensor literature","volume":"24","author":"Becker","year":"2011","journal-title":"J. Mol. Recognit."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Vashist, S.K., and Vashist, P. (2011). Recent advances in quartz crystal microbalance-based sensors. J. Sens., 571405.","DOI":"10.1155\/2011\/571405"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1002\/jmr.2209","article-title":"A Survey of the 2010 Quartz Crystal Microbalance Literature","volume":"25","author":"Speight","year":"2012","journal-title":"J. Mol. Recognit."},{"key":"ref_21","unstructured":"Hosu, O., Selvolini, G., Cristea, C., and Marrazza, G. (2017). Electrochemical Immunosensors for Disease Detection and Diagnosis. Curr. Med. Chem., 24."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1088\/0957-0233\/14\/11\/005","article-title":"Immunosensors using a quartz crystal microbalance","volume":"14","author":"Kurosawa","year":"2003","journal-title":"Meas. Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0376-7388(00)82408-4","article-title":"Determination of microbes and immunoglobulins using a piezoelectric biosensor","volume":"41","author":"Muramatsu","year":"1989","journal-title":"J. Membr. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.snb.2015.04.027","article-title":"Rapid sensing of hepatitis B virus using QCM in the thickness shear mode","volume":"216","author":"Dultseva","year":"2015","journal-title":"Sens. Actuators B"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0956-5663(03)00254-9","article-title":"A self-assembled monolayer-based piezoelectric immunosensor for rapid detection of Escherichia coli O157:H7","volume":"19","author":"Su","year":"2004","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1631\/jzus.B0710307","article-title":"Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor","volume":"9","author":"Wang","year":"2008","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"045004","DOI":"10.1088\/2043-6262\/5\/4\/045004","article-title":"Quartz crystal microbalance (QCM) as biosensor for the detecting of Escherichia coli O157:H7","volume":"5","author":"Ngo","year":"2014","journal-title":"Adv. Nat. Sci. Nanosci. Nanotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0956-5663(02)00166-5","article-title":"Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organism (GMOs) detection","volume":"18","author":"Mannelli","year":"2003","journal-title":"Biosens. Bioelectron."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.snb.2006.02.024","article-title":"In Situ QCM DNA-biosensor probe modification","volume":"120","author":"Lazerges","year":"2006","journal-title":"Sens. Actuators B"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.bios.2006.06.030","article-title":"Quartz crystal microbalance immunosensors for environmental monitoring","volume":"22","author":"Kurosawa","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6463","DOI":"10.1007\/s00216-017-0591-4","article-title":"A high-throughput QCM chip configuration for the study of living cells and cell-drug interactions","volume":"409","author":"Shen","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.bios.2015.11.033","article-title":"Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM sensor","volume":"78","author":"Masdor","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bios.2007.03.007","article-title":"A novel multi-array immunoassay device for tumor markers based on insert-plug model of piezoelectric immunosensor","volume":"23","author":"Zhang","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4161\/org.7.2.15781","article-title":"The role of multipotent marrow stromal cells (MSCs) in tissue regeneration","volume":"7","author":"Westenfelder","year":"2011","journal-title":"Organogenesis"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/scrt37","article-title":"Stem cells for the treatment of neurodegenerative diseases","volume":"1","author":"Dantuma","year":"2010","journal-title":"Stem Cell Res. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/ncb0901-778","article-title":"Isolation of multipotent adult stem cells from the dermis of mammalian skin","volume":"3","author":"Toma","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"S745","DOI":"10.1067\/mai.2003.133","article-title":"Embryonic and adult stem cell therapy","volume":"111","author":"Henningson","year":"2003","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.15537\/smj.2015.12.12750","article-title":"Dental pulp stem cells","volume":"36","author":"Ashri","year":"2015","journal-title":"Saudi Med. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"839","DOI":"10.4252\/wjsc.v7.i5.839","article-title":"Human dental pulp stem cells: Applications in future regenerative medicine","volume":"26","author":"Potdar","year":"2015","journal-title":"World J. Stem Cells"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1177\/154405910508401105","article-title":"NF-kB Activation in human dental pulp stem cells by TNF and LPS","volume":"84","author":"Chang","year":"2005","journal-title":"J. Dent. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.bios.2017.10.028","article-title":"Label-free biosensors in the field of stem cell biology","volume":"101","author":"Fathi","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10561-017-9658-x","article-title":"Mesenchymal stromal\/stem cell separation methods: Concise review","volume":"18","author":"Nicodemou","year":"2017","journal-title":"Cell Tissue Bank"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"15947","DOI":"10.3390\/s121115947","article-title":"Overview of Micro- and Nano-Technology Tools for Stem Cell Applications: Micropatterned and Microelectronic Devices","volume":"12","author":"Cagnin","year":"2012","journal-title":"Sensors"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-1-61737-960-4_10","article-title":"Characterization of human adipose-derived stem cells using flow cytometry","volume":"702","author":"Tucker","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0952-7915(91)90058-9","article-title":"Flow cytometry and cell-separation procedures","volume":"3","author":"Battye","year":"1991","journal-title":"Curr. Opin. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1093\/clinchem\/48.10.1819","article-title":"The history and future of the fluorescence activated cell sorter and flow cytometry: A view from Stanford","volume":"48","author":"Herzenberg","year":"2002","journal-title":"Clin. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0167-5699(00)01678-9","article-title":"Monoclonal antibodies and the FACS: Complementary tools for immunobiology and medicine)","volume":"21","author":"Herzenberg","year":"2000","journal-title":"Immunol. Today"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1088\/0034-4885\/60\/11\/005","article-title":"Biosensors: Recent advances","volume":"60","author":"Collings","year":"1997","journal-title":"Rep. Prog. Phys."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1021\/bm061197b","article-title":"Protein Immobilization Strategies for Protein Biochips","volume":"8","author":"Rusmini","year":"2007","journal-title":"Biomacromolecules"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4431","DOI":"10.1039\/c1an15325k","article-title":"Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay","volume":"136","author":"Vashist","year":"2011","journal-title":"Analyst"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"11083","DOI":"10.1021\/cr5000943","article-title":"Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics","volume":"114","author":"Vashist","year":"2014","journal-title":"Chem. Rev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/clinchem\/37.5.625","article-title":"The Biotin-(Strept)Avidin System: Principles and Applications in Biotechnology","volume":"37","author":"Diamandis","year":"1991","journal-title":"Clin. Chem."},{"key":"ref_53","unstructured":"Savage, M.D., Mattson, G., Desai, S., Nielander, G.W., Morgensen, S., and Conklin, E.J. (1992). Avidin-Biotin Chemistry: A Handbook, Pierce Chemical Co."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1021\/cr9502357","article-title":"Formation and structure of self-assembled monolayers","volume":"96","author":"Ulman","year":"1996","journal-title":"Chem. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"43R","DOI":"10.1039\/a606964i","article-title":"Self-assembled monolayers for biosensors","volume":"122","author":"Wink","year":"1997","journal-title":"Analyst"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Vestergaard, M.C., Kerman, K., Hsing, I.-M., and Tamiya, E. (2015). Introduction to Nanobiosensors and Nanobioanalyses. Nanobiosensors and Nanobioanalyses, Springer.","DOI":"10.1007\/978-4-431-55190-4"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1146\/annurev.physchem.52.1.107","article-title":"Mechanisms and kinetics of self-assembled monolayer formation","volume":"52","author":"Schwartz","year":"2001","journal-title":"Annu. Rev. Phys. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"6386","DOI":"10.1021\/la904087s","article-title":"Characterization of Streptavidin Binding to Biotinylated, Binary Self-Assembled Thiol Monolayers. Influence of component ratio and solvent","volume":"26","author":"Seifert","year":"2010","journal-title":"Langmuir"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1006\/jcis.2000.7362","article-title":"Binding of Biotin to Gold Surfaces Functionalized by Self-Assembled Monolayers of Cystamine and Cysteamine: Combined FT-IRRAS and XPS Characterization","volume":"235","author":"Yam","year":"2001","journal-title":"J. Colloid Interface Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"13499","DOI":"10.1021\/jp076707q","article-title":"Supramolecular architectures of streptavidin on biotinylated self-assembled monolayers. Tracking biomolecular reorganization after bioconjugation","volume":"111","author":"Azzaroni","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1021\/ac00020a015","article-title":"Characterization of a Quartz Crystal Microbalance with Simultaneous Mass and Liquid Loading","volume":"63","author":"Martin","year":"1991","journal-title":"Anal. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1039\/C5AY02898A","article-title":"Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors","volume":"8","author":"Atay","year":"2016","journal-title":"Anal. Methods"},{"key":"ref_63","unstructured":"Lodish, H., Berk, A., Zipursky, S.L., Matsudaira, P., Baltimore, D., and Darnell, J. (2017, November 12). The Molecules of Life, Molecular Cell Biology, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK21473\/."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.tibtech.2015.09.001","article-title":"Emerging Technologies for Next-Generation Point-of-Care Testing","volume":"33","author":"Vashist","year":"2015","journal-title":"Trends Biotechnol."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/12\/2747\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:51:40Z","timestamp":1760208700000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/12\/2747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,28]]},"references-count":64,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["s17122747"],"URL":"https:\/\/doi.org\/10.3390\/s17122747","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,28]]}}}