{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:05:58Z","timestamp":1768259158691,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T00:00:00Z","timestamp":1514160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["16-15-00245"],"award-info":[{"award-number":["16-15-00245"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, a comparative theoretical and experimental analysis of two immuno-chromatographic serodiagnostics schemes, which differ in the immobilization of immunoreagents and the order of the formation of immune complexes, is performed. Based on the theoretical models, the assays are characterized to determine which scheme has a higher quantity of the detected complex and thus ensures the sensitivity of the analysis. The results show that for the effective detection of low-affinity antibodies, the scheme involving the immobilization of the antigen on gold nanoparticles and the antibody-binding protein on the test strip was more sensitive than the predominantly used scheme, which inverts the immunoreagents\u2019 locations. The theoretical predictions were confirmed by the experimental testing of sera collected from tuberculosis patients.<\/jats:p>","DOI":"10.3390\/s18010036","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T03:06:38Z","timestamp":1514257598000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Theoretical and Experimental Comparison of Different Formats of Immunochromatographic Serodiagnostics"],"prefix":"10.3390","volume":"18","author":[{"given":"Dmitriy","family":"Sotnikov","sequence":"first","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3008-2839","authenticated-orcid":false,"given":"Anatoly","family":"Zherdev","sequence":"additional","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4008-4918","authenticated-orcid":false,"given":"Boris","family":"Dzantiev","sequence":"additional","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,25]]},"reference":[{"key":"ref_1","unstructured":"Wild, D. (2005). The Immunoassay Handbook, Elsevier Ltd.. [3rd ed.]."},{"key":"ref_2","unstructured":"Parija, S.C. (2012). Textbook of Microbiology & Immunology\u2014E-Book, Reed Elsevier India Pvt. Ltd.. [2nd ed.]."},{"key":"ref_3","unstructured":"Tille, P. (2013). Bailey & Scott\u2019s Diagnostic Microbiology\u2014E-Book, Elsevier Ltd.. [13th ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.clinbiochem.2005.03.008","article-title":"Point-of-care immunotesting: Approaching the analytical performance of central laboratory methods","volume":"38","year":"2005","journal-title":"J. Clin. 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