{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:00:16Z","timestamp":1782799216506,"version":"3.54.5"},"reference-count":51,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T00:00:00Z","timestamp":1514246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 \u00d7 10\u22126\u20131 \u00d7 10\u22128 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10\u22128 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007\/s00604-017-2586-4).<\/jats:p>","DOI":"10.3390\/s18010049","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T11:13:23Z","timestamp":1514286803000},"page":"49","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Amplified Detection of the Aptamer\u2013Vanillin Complex with the Use of Bsm DNA Polymerase"],"prefix":"10.3390","volume":"18","author":[{"given":"Mariia","family":"Andrianova","sequence":"first","affiliation":[{"name":"Scientific-Manufacturing Complex Technological Centre, 1\u20137 Shokin Square, Zelenograd, 124498 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Komarova","sequence":"additional","affiliation":[{"name":"Scientific-Manufacturing Complex Technological Centre, 1\u20137 Shokin Square, Zelenograd, 124498 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaliy","family":"Grudtsov","sequence":"additional","affiliation":[{"name":"Scientific-Manufacturing Complex Technological Centre, 1\u20137 Shokin Square, Zelenograd, 124498 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniy","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"Scientific-Manufacturing Complex Technological Centre, 1\u20137 Shokin Square, Zelenograd, 124498 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1333-5294","authenticated-orcid":false,"given":"Alexander","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"Scientific-Manufacturing Complex Technological Centre, 1\u20137 Shokin Square, Zelenograd, 124498 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1007\/s00216-007-1735-8","article-title":"Selection of fluorescent aptamer beacons that light up in the presence of zinc","volume":"390","author":"Rajendran","year":"2008","journal-title":"Anal. 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