{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T21:45:06Z","timestamp":1762033506958,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T00:00:00Z","timestamp":1517184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.<\/jats:p>","DOI":"10.3390\/s18020385","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T07:46:20Z","timestamp":1517211980000},"page":"385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins"],"prefix":"10.3390","volume":"18","author":[{"given":"Huan","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"},{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}]},{"given":"Bibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"},{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}]},{"given":"Shuwen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"},{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}]},{"given":"Chunyan","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"},{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2957-0165","authenticated-orcid":false,"given":"Ying","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"},{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"}]},{"given":"Yuyang","family":"Jiang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China"},{"name":"School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China"}]}],"member":"1968","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4252","DOI":"10.1039\/C6CS00011H","article-title":"The principle of conformational signaling","volume":"45","author":"Tompa","year":"2016","journal-title":"Chem. Soc. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.aspen.2015.07.005","article-title":"Expression patterns of five heat shock proteins in Sesamia inferens (Lepidoptera: Noctuidae) during heat stress","volume":"18","author":"Tang","year":"2015","journal-title":"J. Asia Pac. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.aca.2017.02.040","article-title":"An immunochromatographic assay for carcinoembryonic antigen on cotton thread using a composite of carbon nanotubes and gold nanoparticles as reporters","volume":"969","author":"Jia","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.aca.2016.06.043","article-title":"Aberrant sialylation of a prostate-specific antigen: Electrochemical label-free glycoprofiling in prostate cancer serum samples","volume":"934","author":"Pihikova","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.aca.2016.02.026","article-title":"Versatile microfluidic complement fixation test for disease biomarker detection","volume":"916","author":"Li","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1039\/C4CS00370E","article-title":"Cancer biomarker detection: Recent achievements and challenges","volume":"44","author":"Wu","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.aca.2017.03.034","article-title":"An ultra-specific and sensitive sandwich ELISA for imatinib using two anti-imatinib antibodies","volume":"969","author":"Saita","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.aca.2017.01.051","article-title":"Biofunctional polyelectrolytes assembling on biosensors\u2014A versatile surface coating method for protein detections","volume":"964","author":"Han","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.aca.2016.09.030","article-title":"Rapid detection of bacterial endotoxins in ophthalmic viscosurgical device materials by direct analysis in real time mass spectrometry","volume":"943","author":"Li","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.aca.2015.08.052","article-title":"A novel electrochemical immunosensor using \u03b2-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase as labels for ultrasensitive detection of alpha-fetoprotein","volume":"893","author":"Gao","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1039\/C3AN02329J","article-title":"Measuring masses of large biomolecules and bioparticles using mass spectrometric techniques","volume":"139","author":"Peng","year":"2014","journal-title":"Analyst"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6639","DOI":"10.1039\/c3an01567j","article-title":"Dual signal amplification strategy for the fabrication of an ultrasensitive electrochemiluminescenct aptasensor","volume":"138","author":"Zhao","year":"2013","journal-title":"Analyst"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.aca.2016.11.033","article-title":"Graphene oxide directed in-situ deposition of electroactive silver nanoparticles and its electrochemical sensing application for DNA analysis","volume":"951","author":"Gao","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aca.2017.01.052","article-title":"Hybrid monolith sorbent of polypyrrole-coated graphene oxide incorporated into a polyvinyl alcohol cryogel for extraction and enrichment of sulfonamides from water samples","volume":"961","author":"Chullasat","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7069","DOI":"10.1021\/am302704a","article-title":"Graphene Oxide as a Quencher for Fluorescent Assay of Amino Acids, Peptides, and Proteins","volume":"4","author":"Li","year":"2012","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10680","DOI":"10.1039\/c1cc13975d","article-title":"A graphene oxide-peptide fluorescence sensor tailor-made for simple and sensitive detection of matrix metalloproteinase 2","volume":"47","author":"Feng","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7065","DOI":"10.1002\/anie.201101351","article-title":"Graphene oxide-peptide conjugate as an intracellular protease sensor for caspase-3 activation imaging in live cells","volume":"50","author":"Wang","year":"2011","journal-title":"Angew. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.canlet.2014.05.002","article-title":"Peptide-based cancer therapy: Opportunity and challenge","volume":"351","author":"Wu","year":"2014","journal-title":"Cancer Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1039\/C0CC04887A","article-title":"Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity","volume":"47","author":"Zhang","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1021\/am2000104","article-title":"A new fluorescence turn-on assay for trypsin and inhibitor screening based on graphene oxide","volume":"3","author":"Gu","year":"2011","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/nnano.2007.451","article-title":"Processable aqueous dispersions of graphene nanosheets","volume":"3","author":"Li","year":"2008","journal-title":"Nat. Nanotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4477","DOI":"10.1021\/jp9731821","article-title":"Structure of Graphite Oxide RevisitedII","volume":"102","author":"Lerf","year":"1998","journal-title":"J. Phys. Chem. B"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7918","DOI":"10.1039\/C5AN01759A","article-title":"Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform","volume":"140","author":"Li","year":"2015","journal-title":"Analyst"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.1039\/C4AN00151F","article-title":"An enzyme-aided amplification strategy for sensitive detection of DNA utilizing graphene oxide (GO) as a fluorescence quencher","volume":"139","author":"Zhang","year":"2014","journal-title":"Analyst"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e1001588","DOI":"10.1371\/journal.pbio.1001588","article-title":"Control of cellular Bcl-xL levels by deamidation-regulated degradation","volume":"11","author":"Dho","year":"2013","journal-title":"PLoS Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.abb.2009.01.003","article-title":"A conserved hydrophobic core at Bcl-xL mediates its structural stability and binding affinity with BH3-domain peptide of pro-apoptotic protein","volume":"484","author":"Yu","year":"2009","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.pep.2007.04.019","article-title":"Expression, purification and characterization of human urodilatin in E. coli","volume":"55","author":"Sun","year":"2007","journal-title":"Protein Expr. Purif."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.bbrc.2016.01.158","article-title":"Proteolysis targeting peptide (PROTAP) strategy for protein ubiquitination and degradation","volume":"470","author":"Zheng","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"42443","DOI":"10.1039\/C6RA07310G","article-title":"A sensitive polymeric dark quencher-based sensing platform for fluorescence \u201cturn on\u201d detection of proteins","volume":"6","author":"Lv","year":"2016","journal-title":"RSC Adv."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1039\/c2nr12061e","article-title":"A graphene oxide-based fluorescent aptasensor for the turn-on detection of epithelial tumor marker mucin 1","volume":"4","author":"He","year":"2012","journal-title":"Nanoscale"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1039\/C5SC03909F","article-title":"Two-color Imaging of MicroRNA with Enzyme-Free Signal Amplification via Hybridization Chain Reactions in Living Cells","volume":"7","author":"Li","year":"2016","journal-title":"Chem. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5230","DOI":"10.1021\/ac049565u","article-title":"Signaling Aptamer\/Protein Binding by a Molecular Light Switch Complex","volume":"76","author":"Jiang","year":"2004","journal-title":"Anal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/2\/385\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:52:54Z","timestamp":1760194374000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/2\/385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,29]]},"references-count":32,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2]]}},"alternative-id":["s18020385"],"URL":"https:\/\/doi.org\/10.3390\/s18020385","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2018,1,29]]}}}