{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T05:13:28Z","timestamp":1776489208198,"version":"3.51.2"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,2,3]],"date-time":"2018-02-03T00:00:00Z","timestamp":1517616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["NRF- 2017R1C1B2009460"],"award-info":[{"award-number":["NRF- 2017R1C1B2009460"]}]},{"name":"Railway Technology Research Project of the Ministry of Land Infrastructure and Transport","award":["17RTRP-B082486-04"],"award-info":[{"award-number":["17RTRP-B082486-04"]}]},{"name":"Mid-career Researcher Support Program","award":["2015R1A2A1A01005393"],"award-info":[{"award-number":["2015R1A2A1A01005393"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol\u2013gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2\u2032-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex\u00ae UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 \u03bcM to 1 mM with a lower detection limit of 5 \u03bcM. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules.<\/jats:p>","DOI":"10.3390\/s18020457","type":"journal-article","created":{"date-parts":[[2018,2,5]],"date-time":"2018-02-05T04:29:42Z","timestamp":1517804982000},"page":"457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection"],"prefix":"10.3390","volume":"18","author":[{"given":"Han","family":"Song","sequence":"first","affiliation":[{"name":"Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, Korea"}]},{"given":"Yongil","family":"Lee","sequence":"additional","affiliation":[{"name":"Korea Railroad Research Institute (KRRI), 176 Cheoldobakmulkwan-ro, Uiwang-si, Gyeonggi-do 16105, Korea"},{"name":"Department of Mechanical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Korea"}]},{"given":"Vu","family":"Bui","sequence":"additional","affiliation":[{"name":"Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, Korea"}]},{"given":"You-Kwon","family":"Oh","sequence":"additional","affiliation":[{"name":"School of Chemical and Biomolecular Engineering, Pusan National University, Busan 46241, Korea"}]},{"given":"Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1844-0939","authenticated-orcid":false,"given":"Moon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4702-0282","authenticated-orcid":false,"given":"Young-Chul","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2018,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1039\/C2CS35265F","article-title":"Design, functionalization strageties and biomedical applications of targeted biodegradable\/biocompatible polymer-based nanocarriers for drug delivery","volume":"42","author":"Nicolas","year":"2013","journal-title":"Chem. 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