{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:00:36Z","timestamp":1777442436113,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T00:00:00Z","timestamp":1521072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing process, resulting in a strong association with high bioluminescence signal onto a NeutrAvidin-coated surface. When the peptide substrate for MMP-7 was inserted into a region between luciferase and intein, the biotinylated probe detected MMP-7 activity by cleaving the peptide, and surface-induced bioluminescence signal was strongly reduced in the MMP-secreted media or mouse tissue extracts, compared with that in MMP-deficient control set. Our approach is anticipated to be useful for generating biotinylated proteins and for their applications in diagnosing MMP activity in human diseases.<\/jats:p>","DOI":"10.3390\/s18030875","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T16:07:17Z","timestamp":1521130037000},"page":"875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase"],"prefix":"10.3390","volume":"18","author":[{"given":"Duc","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"},{"name":"Brain Korea 21 Plus BioDefense Research Team, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanim","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dasom","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"},{"name":"Brain Korea 21 Plus BioDefense Research Team, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung","family":"Gye","sequence":"additional","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"},{"name":"Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7234-1320","authenticated-orcid":false,"given":"Young-Pil","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Hanyang University, Seoul 04763, Korea"},{"name":"Brain Korea 21 Plus BioDefense Research Team, Hanyang University, Seoul 04763, Korea"},{"name":"Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea"},{"name":"Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1582-4934.2006.tb00398.x","article-title":"The story of cell secretion: Events leading to the discovery of the \u2019porosome\u2019\u2014The universal secretory machinery in cells","volume":"10","author":"Jeftinija","year":"2006","journal-title":"J. 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