{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T18:27:23Z","timestamp":1773167243409,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T00:00:00Z","timestamp":1522108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sardinia Regional Government","award":["CRP-59886"],"award-info":[{"award-number":["CRP-59886"]}]},{"DOI":"10.13039\/501100003407","name":"MIUR","doi-asserted-by":"publisher","award":["NANOMAX - ENCODER"],"award-info":[{"award-number":["NANOMAX - ENCODER"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, the electronic transduction of DNA hybridization is presented by coupling organic charge-modulated field-effect transistors (OCMFETs) and hairpin-shaped probes. These probes have shown interesting properties in terms of sensitivity and selectivity in other kinds of assays, in the form of molecular beacons (MBs). Their integration with organic-transistor based sensors, never explored before, paves the way to a new class of low-cost, easy-to-use, and portable genetic sensors with enhanced performances. Thanks to the peculiar characteristics of the employed sensor, measurements can be performed at relatively high ionic strengths, thus optimizing the probes\u2019 functionality without affecting the detection ability of the device. A complete electrical characterization of the sensor is reported, including calibration with different target concentrations in the measurement environment and selectivity evaluation. In particular, DNA hybridization detection for target concentration as low as 100 pM is demonstrated.<\/jats:p>","DOI":"10.3390\/s18040990","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T12:17:24Z","timestamp":1522153044000},"page":"990","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8451-6132","authenticated-orcid":false,"given":"Corrado","family":"Napoli","sequence":"first","affiliation":[{"name":"Department of Electrical and Electronic Engineering, Universit\u00e0 di Cagliari, 09123 Cagliari, Italy"}]},{"given":"Stefano","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Engineering, Universit\u00e0 di Cagliari, 09123 Cagliari, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0002-2977","authenticated-orcid":false,"given":"Ambra","family":"Giannetti","sequence":"additional","affiliation":[{"name":"Istituto di Fisica Applicata \u201cNello Carrara\u201d, Consiglio Nazionale delle Ricerche, 50019 Sesto Fiorentino, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1660-9833","authenticated-orcid":false,"given":"Sara","family":"Tombelli","sequence":"additional","affiliation":[{"name":"Istituto di Fisica Applicata \u201cNello Carrara\u201d, Consiglio Nazionale delle Ricerche, 50019 Sesto Fiorentino, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2689-5952","authenticated-orcid":false,"given":"Francesco","family":"Baldini","sequence":"additional","affiliation":[{"name":"Istituto di Fisica Applicata \u201cNello Carrara\u201d, Consiglio Nazionale delle Ricerche, 50019 Sesto Fiorentino, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6136-7664","authenticated-orcid":false,"given":"Massimo","family":"Barbaro","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Engineering, Universit\u00e0 di Cagliari, 09123 Cagliari, Italy"}]},{"given":"Annalisa","family":"Bonfiglio","sequence":"additional","affiliation":[{"name":"Department of Electrical and Electronic Engineering, Universit\u00e0 di Cagliari, 09123 Cagliari, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.bios.2017.07.010","article-title":"Detection principles of biological and chemical FET sensors","volume":"98","author":"Kaisti","year":"2017","journal-title":"Biosens. 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