{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:00:43Z","timestamp":1792717243703,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T00:00:00Z","timestamp":1522800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Thiabendazole is widely used in sclerotium blight, downy mildew and black rot prevention and treatment in rape. Accurate monitoring of thiabendazole pesticides in plants will prevent potential adverse effects to the Environment and human health. Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive fingerprint with the advantages of simple operation, convenient portability and high detection efficiency. In this paper, a rapid determination method of thiabendazole pesticides in rape was conducted combining SERS with chemometric methods. The original SERS were pretreated and the partial least squares (PLS) was applied to establish the prediction model between SERS and thiabendazole pesticides in rape. As a result, the SERS enhancing effect based on silver Nano-substrate was better than that of gold Nano-substrate, where the detection limit of thiabendazole pesticides in rape could reach 0.1 mg\/L. Moreover, 782, 1007 and 1576 cm\u22121 could be determined as thiabendazole pesticides Raman characteristic peaks in rape. The prediction effect of thiabendazole pesticides in rape was the best (     R p 2     = 0.94, RMSEP = 3.17 mg\/L) after the original spectra preprocessed with 1st-Derivative, and the linear relevance between thiabendazole pesticides concentration and Raman peak intensity at 782 cm\u22121 was the highest (R2 = 0.91). Furthermore, five rape samples with unknown thiabendazole pesticides concentration were used to verify the accuracy and reliability of this method. It was showed that prediction relative standard deviation was 0.70\u20139.85%, recovery rate was 94.71\u2013118.92% and t value was \u22121.489. In conclusion, the thiabendazole pesticides in rape could be rapidly and accurately detected by SERS, which was beneficial to provide a rapid, accurate and reliable scheme for the detection of pesticides residues in agriculture products.<\/jats:p>","DOI":"10.3390\/s18041082","type":"journal-article","created":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T06:45:37Z","timestamp":1522824337000},"page":"1082","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Rapid Determination of Thiabendazole Pesticides in Rape by Surface Enhanced Raman Spectroscopy"],"prefix":"10.3390","volume":"18","author":[{"given":"Lei","family":"Lin","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7432-8899","authenticated-orcid":false,"given":"Tao","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Nie","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"},{"name":"State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6752-1757","authenticated-orcid":false,"given":"Yong","family":"He","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingquan","family":"Chu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shupei","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Key Laboratory of Sensors Sensing, Ministry of Agriculture, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0021-9673(01)01395-4","article-title":"Analysis of thiabendazole and procymidone in fruits and vegetables by capillary electrophoresis-electrospray mass spectrometry","volume":"949","author":"Font","year":"2002","journal-title":"J. Chromatogr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6347","DOI":"10.1021\/jf0346298","article-title":"Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC)","volume":"51","author":"Rong","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4034","DOI":"10.1002\/anie.200805693","article-title":"A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase","volume":"48","author":"Yi","year":"2009","journal-title":"Angew. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2828","DOI":"10.1016\/S0021-9258(19)45285-X","article-title":"Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates","volume":"247","author":"Hugli","year":"1972","journal-title":"J. Biol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/elps.200500527","article-title":"Recent developments in capillary electrophoresis and capillary electrochromatography of peptides","volume":"27","author":"Kasicka","year":"2006","journal-title":"Electrophoresis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S414","DOI":"10.17221\/1100-CJFS","article-title":"Pesticides in food-immunochromatographic detection of thiabendazole and methiocarb","volume":"27","author":"Fukal","year":"2009","journal-title":"Czech J. Food Sci."},{"key":"ref_7","first-page":"769","article-title":"Determination of residues of carbendazim and thiabendazole pesticides in fruits by dispersive solid-phase extraction (dispersive SPE) and HPLC","volume":"25","author":"Chen","year":"2008","journal-title":"J. Fruit Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1016\/j.foodchem.2006.08.028","article-title":"A review: Current analytical methods for the determination of biogenic amines in foods","volume":"103","year":"2007","journal-title":"Food Chem."},{"key":"ref_9","first-page":"1102","article-title":"Enhancement factor distribution around a single surface-enhanced Raman scattering hot spot and its relation to single molecule detection","volume":"125","author":"Ru","year":"2006","journal-title":"J. Chem. Phys."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/jp011730b","article-title":"Single-molecule and single-nanoparticle SERS: Examining the roles of surface active sites and chemical enhancement","volume":"106","author":"Doering","year":"2002","journal-title":"J. Phys. Chem. B"},{"key":"ref_11","first-page":"404","article-title":"Surface-enhanced raman spectroscopy analysis of thiabendazole pesticide","volume":"35","author":"Lin","year":"2015","journal-title":"Spectrosc. Spectr. Anal."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.foodcont.2016.04.003","article-title":"Surface-enhanced raman spectroscopy coupled with gold nanoparticles for rapid detection of phosmet and thiabendazole residues in apples","volume":"68","author":"Luo","year":"2016","journal-title":"Food Control"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.foodchem.2017.07.014","article-title":"Determination of thiabendazole in orange juice using an MISPE-SERS chemosensor","volume":"239","author":"Feng","year":"2018","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.foodchem.2013.10.023","article-title":"Recovery and quantitative detection of thiabendazole on apples using a surface swab capture method followed by surface-enhanced raman spectroscopy","volume":"148","author":"He","year":"2014","journal-title":"Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1002\/smll.200800337","article-title":"Nanostructured surfaces and assemblies as SERS media","volume":"4","author":"Ko","year":"2008","journal-title":"Small"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"56240","DOI":"10.1039\/C6RA08000F","article-title":"Magnetic solid-phase extraction based on ferroferric oxide nanoparticles doubly coated with chitosan and \u03b2-cyclodextrin in layer-by-layer mode for the separation of ibuprofen","volume":"6","author":"Chen","year":"2016","journal-title":"RSC Adv."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4450","DOI":"10.1016\/j.apsusc.2006.09.069","article-title":"An investigation of the surface-enhanced raman scattering (SERS) effect from laser irradiation of ag nanoparticles prepared by trisodium citrate reduction method","volume":"253","author":"Ding","year":"2007","journal-title":"Appl. Surf. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1021\/am200057f","article-title":"A reproducible SERS substrate based on electrostatically assisted aptes-functionalized surface-assembly of gold nanostars","volume":"3","author":"Su","year":"2011","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1007\/s00216-009-3331-6","article-title":"Applications of LC\/ESI-MS\/MS and UHPLC QqTOF MS for the determination of 148 pesticides in fruits and vegetables","volume":"396","author":"Wang","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1039\/b708459p","article-title":"SERS\u2014A single-molecule and nanoscale tool for bioanalytics","volume":"37","author":"Kneipp","year":"2008","journal-title":"Chem. Soc. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.chemolab.2008.11.006","article-title":"On the geometry of SNV and MSC","volume":"96","author":"Fearn","year":"2009","journal-title":"Chemom. Intell. Lab. Syst."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/bioinformatics\/19.2.185","article-title":"A comparison of normalization methods for high density oligonucleotide array data based on variance and bias","volume":"19","author":"Bolstad","year":"2003","journal-title":"Bioinformatics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1366\/0003702884429869","article-title":"The effect of multiplicative scatter correction (MSC) and linearity improvement in NIR spectroscopy","volume":"42","author":"Isaksson","year":"1988","journal-title":"Appl. Spectrosc."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Nie, P., Tao, D., Yong, H., and Qu, F. (2017). Detection of soil nitrogen using near infrared sensors based on soil pretreatment and algorithms. Sensors, 17.","DOI":"10.3390\/s17051102"},{"key":"ref_25","first-page":"262","article-title":"The local effect of partial least squares regression modeling parameters on near infrared detection results of","volume":"02","author":"Li","year":"2007","journal-title":"J. Spectrosc. Spectr. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1111\/j.1743-6109.2009.01351.x","article-title":"Erectile function recovery rate after radical prostatectomy: A meta-analysis","volume":"6","author":"Tal","year":"2009","journal-title":"J. Sex. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kawamura, K., Tsujimoto, Y., Rabenarivo, M., Asai, H., Andriamananjara, A., and Rakotoson, T. (2017). Vis-NIR spectroscopy and PLS regression with waveband selection for estimating the total C and N of paddy soils in Madagascar. Remote Sens., 9.","DOI":"10.3390\/rs9101081"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Blanchard, P., and Br\u00fcning, E. (2015). Mathematical Methods in Physics, Birkh\u00e4user.","DOI":"10.1007\/978-3-319-14045-2"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1002\/(SICI)1096-987X(19990115)20:1<129::AID-JCC13>3.0.CO;2-A","article-title":"Chemical reactivity indexes in density functional theory","volume":"20","author":"Chermette","year":"2015","journal-title":"J. Comput. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1002\/jcc.24350","article-title":"Highly efficient implementation of pseudospectral time-dependent density-functional theory for the calculation of excitation energies of large molecules","volume":"37","author":"Cao","year":"2016","journal-title":"J. Comput. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.theochem.2009.07.036","article-title":"Excited-state spin-contamination in time-dependent density-functional theory for molecules with open-shell ground states","volume":"914","author":"Ipatov","year":"2009","journal-title":"J. Mol. Struct. Theochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.molstruc.2013.07.017","article-title":"Using solid-state density functional theory and terahertz spectroscopy to spectroscopically distinguish the various hydrohalide salts of 5-(4-pyridyl) tetrazole","volume":"1050","author":"Pellizzeri","year":"2013","journal-title":"J. Mol. Struct."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"28647","DOI":"10.1039\/C7CP05544G","article-title":"Quantification of cation-anion interactions in crystalline monopotassium and monosodium glutamate salts","volume":"19","author":"Ruggiero","year":"2017","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4285","DOI":"10.1021\/la0341815","article-title":"Mercaptoacetic acid-capped silver nanoparticles colloid:\u2009 Formation, morphology, and SERS activity","volume":"19","author":"Xiao","year":"2003","journal-title":"Langmuir"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1021\/ac00255a014","article-title":"Partial least-squares method for spectrofluorimetric analysis of mixtures of humic acid and lignin sulfonate","volume":"55","author":"Lindberg","year":"1983","journal-title":"Anal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/4\/1082\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:59:33Z","timestamp":1760194773000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/4\/1082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,4]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,4]]}},"alternative-id":["s18041082"],"URL":"https:\/\/doi.org\/10.3390\/s18041082","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,4]]}}}