{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:33:13Z","timestamp":1776835993694,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T00:00:00Z","timestamp":1523491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Malaysian Ministry of Science, Technology and Innovation","award":["06-01-02-SF1242"],"award-info":[{"award-number":["06-01-02-SF1242"]}]},{"name":"Malaysian Ministry of Science, Technology and Innovation","award":["NND\/ND\/(2)\/TD11-009"],"award-info":[{"award-number":["NND\/ND\/(2)\/TD11-009"]}]},{"name":"National University of Malaysia (UKM)","award":["DIP-2016-028"],"award-info":[{"award-number":["DIP-2016-028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N,N\u2032-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 \u00d7 10\u221216\u20131.0 \u00d7 10\u221210 M (R2 = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes.<\/jats:p>","DOI":"10.3390\/s18041173","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T12:19:27Z","timestamp":1523535567000},"page":"1173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection"],"prefix":"10.3390","volume":"18","author":[{"given":"Eda","family":"Ariffin","sequence":"first","affiliation":[{"name":"School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Tan","sequence":"additional","affiliation":[{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurul","family":"Abd. Karim","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee","family":"Yook Heng","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia"},{"name":"Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.colsurfb.2011.04.028","article-title":"An impedimetric biosensor for detection of dengue serotype at picomolar concentration based on gold nanoparticle-polyaniline hybrid composites","volume":"86","author":"Nascimento","year":"2011","journal-title":"Colloids Surf. 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