{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:31:08Z","timestamp":1772253068839,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T00:00:00Z","timestamp":1526601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Surface Plasmon Resonance (SPR)-based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still mostly limited to prism-coupled devices. Plasmonic gratings, combined with a simple and cost-effective instrumentation, have been poorly developed compared to prism-coupled system mainly due to their lower sensitivity. Here we describe the optimization and signal enhancement of a sensing platform based on phase-interrogation method, which entails the exploitation of a nanostructured sensor. This technique is particularly suitable for integration of the plasmonic sensor in a lab-on-a-chip platform and can be used in a microfluidic chamber to ease the sensing procedures and limit the injected volume. The careful optimization of most suitable experimental parameters by numerical simulations leads to a 30\u201350% enhancement of SPR response, opening new possibilities for applications in the biomedical research field while maintaining the ease and versatility of the configuration.<\/jats:p>","DOI":"10.3390\/s18051621","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T04:07:30Z","timestamp":1526875650000},"page":"1621","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Grating-Coupled Surface Plasmon Resonance (GC-SPR) Optimization for Phase-Interrogation Biosensing in a Microfluidic Chamber"],"prefix":"10.3390","volume":"18","author":[{"given":"Stefano","family":"Rossi","sequence":"first","affiliation":[{"name":"Department of Physics and Astronomy \u201cG. Galilei\u201d, University of Padua, Via Marzolo 8, 35131 Padua, Italy"},{"name":"Laboratory for Nanofabrication of Nanodevices, Corso Stati Uniti 4, 35127 Padua, Italy"},{"name":"Fondazione Institute of Pediatric Research Citt\u00e0 della Speranza, Corso Stati Uniti 4, 35127 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Gazzola","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy \u201cG. Galilei\u201d, University of Padua, Via Marzolo 8, 35131 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Capaldo","sequence":"additional","affiliation":[{"name":"CNR-INFM TASC IOM National Laboratory, Area Science Park S.S. 14 km 163.5, 34012 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Borile","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy \u201cG. Galilei\u201d, University of Padua, Via Marzolo 8, 35131 Padua, Italy"},{"name":"Laboratory for Nanofabrication of Nanodevices, Corso Stati Uniti 4, 35127 Padua, Italy"},{"name":"Fondazione Institute of Pediatric Research Citt\u00e0 della Speranza, Corso Stati Uniti 4, 35127 Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Romanato","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy \u201cG. Galilei\u201d, University of Padua, Via Marzolo 8, 35131 Padua, Italy"},{"name":"Laboratory for Nanofabrication of Nanodevices, Corso Stati Uniti 4, 35127 Padua, Italy"},{"name":"Fondazione Institute of Pediatric Research Citt\u00e0 della Speranza, Corso Stati Uniti 4, 35127 Padua, Italy"},{"name":"CNR-INFM TASC IOM National Laboratory, Area Science Park S.S. 14 km 163.5, 34012 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1021\/cr068107d","article-title":"Surface plasmon resonance sensors for detection of chemical and biological species","volume":"108","author":"Homola","year":"2008","journal-title":"Chem. 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