{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T09:55:11Z","timestamp":1776160511387,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T00:00:00Z","timestamp":1529884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles\/polyaniline composite film-modified electrodes (AuNPs\/PANI\/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 \u03bcM and 10 \u03bcM with a detection limit of 0.27 \u03bcM.<\/jats:p>","DOI":"10.3390\/s18072035","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T11:03:25Z","timestamp":1529924605000},"page":"2035","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9083-074X","authenticated-orcid":false,"given":"Giulia","family":"Selvolini","sequence":"first","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioana","family":"B\u0103jan","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"},{"name":"Department of Analytical Chemistry, University of Medicine and Pharmacy \u201cIuliu Ha\u0163ieganu\u201d, 4 Pasteur Street, 400349 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-5195","authenticated-orcid":false,"given":"Oana","family":"Hosu","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"},{"name":"Department of Analytical Chemistry, University of Medicine and Pharmacy \u201cIuliu Ha\u0163ieganu\u201d, 4 Pasteur Street, 400349 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-3324","authenticated-orcid":false,"given":"Cecilia","family":"Cristea","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Medicine and Pharmacy \u201cIuliu Ha\u0163ieganu\u201d, 4 Pasteur Street, 400349 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"S\u0103ndulescu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Medicine and Pharmacy \u201cIuliu Ha\u0163ieganu\u201d, 4 Pasteur Street, 400349 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2465-7552","authenticated-orcid":false,"given":"Giovanna","family":"Marrazza","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"},{"name":"Istituto Nazionale Biostrutture e Biosistemi (INBB), Unit of Florence, Viale delle Medaglie d\u2019Oro 305, 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,6,25]]},"reference":[{"key":"ref_1","unstructured":"FAO (Food and Agriculture Organization), and WHO (World Health Organization) (2016). 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