{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T20:58:17Z","timestamp":1774385897312,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T00:00:00Z","timestamp":1530835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A spectral integration combined with a threshold method for the analysis of spectral scanning surface plasmon resonance (SPR) images can significantly increase signal recognition at low concentration of antibody solution. The 12-well SPR sensing plates consisted of gold-capped nanowire arrays with 500-nm period, 80-nm linewidth and 50-nm gold thickness which were used for generating multiple SPR images. A threshold method is introduced to eliminate background noises in spectral scanning images. Combining spectral integration and the threshold method, the detection limit of antibody concentration was 1.23 ng\/mL. Using multiple-well SPR sensing plates and the proposed analytical method, multiple kinetic responses with spectral and spatial information on different sensing areas can be sensitively measured.<\/jats:p>","DOI":"10.3390\/s18072181","type":"journal-article","created":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T10:55:44Z","timestamp":1530874544000},"page":"2181","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays"],"prefix":"10.3390","volume":"18","author":[{"given":"Yi-Hsin","family":"Tai","sequence":"first","affiliation":[{"name":"Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Han","family":"Fu","sequence":"additional","affiliation":[{"name":"Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuang-Li","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3002-0526","authenticated-orcid":false,"given":"Pei-Kuen","family":"Wei","sequence":"additional","affiliation":[{"name":"Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan"},{"name":"Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan"},{"name":"Institute of Optoelectronic Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4939","DOI":"10.1021\/ac9709763","article-title":"Surface plasmon resonance imaging measurements of DNA hybridization adsorption and streptavidin\/DNA multilayer formation at chemically modified gold surfaces","volume":"69","author":"Jordan","year":"1997","journal-title":"Anal. 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