{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T19:14:06Z","timestamp":1775157246742,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["268017\/E40"],"award-info":[{"award-number":["268017\/E40"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61650410655"],"award-info":[{"award-number":["61650410655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A novel toxicity-warning sensor for water quality monitoring in recirculating aquaculture systems (RAS) is presented. The design of the sensor system mainly comprises a whole-cell biosensor. Aliivibrio fischeri, a luminescent bacterium widely used in toxicity analysis, was tested for a mixture of known fish-health stressors, namely nitrite, un-ionized ammonia, copper, aluminum and zinc. Two toxicity predictive models were constructed. Correlation, root mean squared error, relative error and toxic behavior were analyzed. The linear concentration addition (LCA) model was found suitable to ally with a machine learning algorithm for prediction of toxic events, thanks to additive behavior near the limit concentrations for these stressors, with a root-mean-squared error (RMSE) of 0.0623, and a mean absolute error of 4%. The model was proved to have a smaller relative deviation than other methods described in the literature. Moreover, the design of a novel microfluidic chip for toxicity testing is also proposed, which is to be integrated in a fluidic system that functions as a bypass of the RAS tank to enable near-real time monitoring. This chip was tested with simulated samples of RAS water spiked with zinc, with an EC50 of 6,46E-7 M. Future work will be extended to the analysis of other stressors with the novel chip.<\/jats:p>","DOI":"10.3390\/s18092848","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T02:49:34Z","timestamp":1535597374000},"page":"2848","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Monitoring Aquaculture Water Quality: Design of an Early Warning Sensor with Aliivibrio fischeri and Predictive Models"],"prefix":"10.3390","volume":"18","author":[{"given":"Lu\u00eds F. B. A.","family":"Da Silva","sequence":"first","affiliation":[{"name":"Institute of Applied Micro-Nano Science and Technology\u2014IAMNST, Chongqing Key Laboratory of Colleges and Universities on Micro-Nano Systems Technology and Smart Transducing, Chongqing Engineering Laboratory for Detection, Control and Integrated System, National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Nan\u2019an District, Chongqing 400067, China"},{"name":"Department of Microsystems\u2014IMS, Faculty of Technology, Natural Sciences and Maritime Sciences, University of South-Eastern Norway, Postboks 235, 3603 Kongsberg, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaochu","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Applied Micro-Nano Science and Technology\u2014IAMNST, Chongqing Key Laboratory of Colleges and Universities on Micro-Nano Systems Technology and Smart Transducing, Chongqing Engineering Laboratory for Detection, Control and Integrated System, National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Nan\u2019an District, Chongqing 400067, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuno M. M.","family":"Pires","sequence":"additional","affiliation":[{"name":"Institute of Applied Micro-Nano Science and Technology\u2014IAMNST, Chongqing Key Laboratory of Colleges and Universities on Micro-Nano Systems Technology and Smart Transducing, Chongqing Engineering Laboratory for Detection, Control and Integrated System, National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Nan\u2019an District, Chongqing 400067, China"},{"name":"Centre for Environmental Radioactivity (CERAD CoE), Norwegian University of Life Sciences (NMBU), Faculty of Environmental Sciences and Natural Resource Management, P.O. Box 5003, NO-1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Applied Micro-Nano Science and Technology\u2014IAMNST, Chongqing Key Laboratory of Colleges and Universities on Micro-Nano Systems Technology and Smart Transducing, Chongqing Engineering Laboratory for Detection, Control and Integrated System, National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Nan\u2019an District, Chongqing 400067, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Christian","family":"Teien","sequence":"additional","affiliation":[{"name":"Centre for Environmental Radioactivity (CERAD CoE), Norwegian University of Life Sciences (NMBU), Faculty of Environmental Sciences and Natural Resource Management, P.O. Box 5003, NO-1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trond","family":"Storebakken","sequence":"additional","affiliation":[{"name":"Faculty of Biosciences, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brit","family":"Salbu","sequence":"additional","affiliation":[{"name":"Centre for Environmental Radioactivity (CERAD CoE), Norwegian University of Life Sciences (NMBU), Faculty of Environmental Sciences and Natural Resource Management, P.O. Box 5003, NO-1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,29]]},"reference":[{"key":"ref_1","unstructured":"(2018, April 14). AQUI-S New Zealand LTD Stress in Fish. 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