{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:03:44Z","timestamp":1778623424355,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T00:00:00Z","timestamp":1543276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["DPI2016-80391-C3-3-R"],"award-info":[{"award-number":["DPI2016-80391-C3-3-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The design of safe stimulation protocols for functional electrostimulation requires knowledge of the \u201cmaximum reversible charge injection capacity\u201d of the implantable microelectrodes. One of the main difficulties encountered in characterizing such microelectrodes is the calculation of the access voltage Va. This paper proposes a method to calculate Va that does not require prior knowledge of the overpotential terms and of the electrolyte (or excitable tissue) resistance, which is an advantage for in vivo electrochemical characterization of microelectrodes. To validate this method, we compare the calculated results with those obtained from conventional methods for characterizing three flexible platinum microelectrodes by cyclic voltammetry and voltage transient measurements. This paper presents the experimental setup, the required instrumentation, and the signal processing.<\/jats:p>","DOI":"10.3390\/s18124152","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T03:31:33Z","timestamp":1543289493000},"page":"4152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A Measurement Setup and Automated Calculation Method to Determine the Charge Injection Capacity of Implantable Microelectrodes"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1556-7179","authenticated-orcid":false,"given":"Ana","family":"Cisnal","sequence":"first","affiliation":[{"name":"Fraunhofer-Institut f\u00fcr Biomedizinische Technik (IBMT), Department of Biomedical Microsystems, 66280 Sulzbach\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2877-7300","authenticated-orcid":false,"given":"Juan-Carlos","family":"Fraile","sequence":"additional","affiliation":[{"name":"ITAP, Universidad de Valladolid, Paseo del Cauce 59, 47011 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7731-2411","authenticated-orcid":false,"given":"Javier","family":"P\u00e9rez-Turiel","sequence":"additional","affiliation":[{"name":"ITAP, Universidad de Valladolid, Paseo del Cauce 59, 47011 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Mu\u00f1oz-Martinez","sequence":"additional","affiliation":[{"name":"Escuela de Ingenier\u00edas Industriales, Universidad de M\u00e1laga, Doctor Ortiz Ramos s\/n, 29071 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Fraunhofer-Institut f\u00fcr Biomedizinische Technik (IBMT), Department of Biomedical Microsystems, 66280 Sulzbach\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2460-8531","authenticated-orcid":false,"given":"Frank","family":"R. 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Neurol."},{"key":"ref_3","first-page":"12","article-title":"Functional electrostimulation in patients with spinal cord injury (scientific review)","volume":"23","year":"2001","journal-title":"Fisioterapia"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/10790268.2017.1390930","article-title":"Comparison of transcutaneous electrical nerve stimulation (TENS) and functional electrical stimulation (FES) for spasticity in spinal cord injury\u2014A pilot randomized cross-over trial","volume":"41","author":"Sivaramakrishnan","year":"2018","journal-title":"J. Spinal Cord. Med."},{"key":"ref_5","first-page":"147","article-title":"Visual prostheses based on direct interfaces with the visual system","volume":"4","author":"Hambrecht","year":"1995","journal-title":"Baillieres Clin. Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5362","DOI":"10.1167\/iovs.02-0817","article-title":"Perceptual efficacy of electrical stimulation of human retina with a microelectrode array during short-term surgical trials","volume":"44","author":"Rizzo","year":"2003","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1126\/science.285.5434.1729","article-title":"Recruitment of the auditory cortex in congenitally deaf cats by long-term cochlear electrostimulation","volume":"285","author":"Klinke","year":"1999","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1098\/rstb.2005.1782","article-title":"The multiple-channel cochlear implant: Interface between the sound and the central nervous system for hearing, speech, and language in deaf people\u2014A personal perspective","volume":"361","author":"Clark","year":"2006","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"G3015","DOI":"10.1149\/2.0031812jes","article-title":"Low-Impedance, High Surface Area Pt-Ir Electrodeposited on Cochlear Implant Electrodes","volume":"165","author":"Lee","year":"2018","journal-title":"J. Electrochem. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"M\u00fcller, E.J., and Robinson, P.A. (2018). Quantitative theory of deep brain stimulation of the subthalamic nucleus for the suppression of pathological rhythms in Parkinson\u2019s disease. PLOS Comput. Biol., 14.","DOI":"10.1371\/journal.pcbi.1006217"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1146\/annurev.neuro.29.051605.112824","article-title":"Deep brain stimulation","volume":"29","author":"Perlmutter","year":"2006","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Itakura, T. (2015). Deep Brain Stimulation for Psychiatric Disorders. Deep Brain Stimulation for Neurological Disorders, Springer.","DOI":"10.1007\/978-3-319-08476-3"},{"key":"ref_13","first-page":"279","article-title":"Functional electrical stimulation: Current status and future prospects of applications to the neuromuscular system in spinal cord injury","volume":"25","author":"Peckham","year":"1987","journal-title":"Paraplegia"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"021001","DOI":"10.1088\/1741-2560\/13\/2\/021001","article-title":"Tissue damage thresholds during therapeutic electrical stimulation","volume":"13","author":"Cogan","year":"2016","journal-title":"J. Neural Eng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev.bioeng.10.061807.160518","article-title":"Neural stimulation and recording electrodes","volume":"10","author":"Cogan","year":"2008","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_16","first-page":"330","article-title":"Neuromuscular electrical stimulation modelling by physiological and black-box approach","volume":"13","year":"2016","journal-title":"Rev. Iberoam. Autom. Inform. Ind."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jneumeth.2004.10.020","article-title":"Electrical stimulation of excitable tissue: Design of efficacious and safe protocols","volume":"141","author":"Merrill","year":"2005","journal-title":"J. Neurosci. Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1109\/TBME.1977.326218","article-title":"Electrochemical considerations for safe electrical stimulation of nervous system with platinum electrodes","volume":"1","author":"Brummer","year":"1977","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.exer.2006.01.012","article-title":"Thresholds for activation of rabbit retinal ganglion cells with a subretinal electrode","volume":"83","author":"Jensen","year":"2006","journal-title":"Exp. Eye Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1152\/jn.2002.88.4.1592","article-title":"Extracellular stimulation of central neurons: Influence of stimulus waveform and frequency on neuronal output","volume":"88","author":"McIntyre","year":"2002","journal-title":"J. Neurophysiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"036023","DOI":"10.1088\/1741-2552\/aa9f31","article-title":"Electrochemical characterization of high frequency stimulation electrodes: Role of electrode material and stimulation parameters on electrode polarization","volume":"15","author":"Ghazavi","year":"2018","journal-title":"J. Neural. Eng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s13246-014-0282-9","article-title":"Electrochemical performance of platinum electrodes within the multi-electrode spiral nerve cuff","volume":"37","author":"Rozman","year":"2014","journal-title":"Australas. Phys. Eng. Sci. Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Brownson, D.A., and Banks, C.E. (2014). Interpreting Electrochemistry in The Handbook of Graphene Electrochemistry, Springer.","DOI":"10.1007\/978-1-4471-6428-9"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gong, C.S.A., Syu, W.J., Lei, K.F., and Hwang, Y.S. (2016). Development of a flexible non-metal electrode for cell stimulation and recording. Sensors, 16.","DOI":"10.3390\/s16101613"},{"key":"ref_25","unstructured":"Bard, A.J., and Faulkner, L.R. (2000). Electrochemical Methods: Fundamentals and Applications, John Wiley & Sons, Inc.. [2nd ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1088\/1741-2560\/5\/1\/006","article-title":"Incorporation of the electrode-electrolyte interface into finite element models of metal microelectrodes","volume":"5","author":"Cantrell","year":"2007","journal-title":"J. Neural Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1073\/pnas.0636434100","article-title":"An approach to electrical modeling of single and multiple cells","volume":"6","author":"Gowrishankar","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","unstructured":"Stenger, D.A., and McKenna, T.M. (1994). Introduction to the theory, design, and modeling of thin-film microelectrodes for neural interfaces. Enabling Technologies for Cultured Neuronal Networks, Academic Press."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1109\/10.775401","article-title":"Modeling the effects of electric field on nerve fibers: Influence of tissue electrical properties","volume":"46","author":"Grill","year":"1999","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1109\/TIM.2013.2253992","article-title":"Fully implantable multi-channel measurement system for acquisition of muscle activity","volume":"62","author":"Lewis","year":"2013","journal-title":"IEEE Instrum. Meas."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0165-0270(00)00192-8","article-title":"Polyimide cuff electrodes for peripheral nerve stimulation","volume":"98","author":"Rodriguez","year":"2000","journal-title":"J. Neurosci. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/BF02368219","article-title":"Electrical Properties of Implant Encapsulation Tissue","volume":"22","author":"Grill","year":"1994","journal-title":"Ann. Biomed. Eng."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1109\/TBME.2014.2366514","article-title":"In vivo and in vitro comparison of the charge injection capacity of platinum macroelectrodes","volume":"62","author":"Leung","year":"2015","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Poppendieck, W., Koch, K., Steltenkamp, S., and Hoffmann, K.P. (2009, January 7\u201312). A measurement set-up to determine the charge injection capacity for neural microelectrodes. Proceedings of the World Congress on Medical Physics and Biomedical Engineering, Munich, Germany.","DOI":"10.1007\/978-3-642-03889-1_44"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1109\/10.61038","article-title":"Electrical stimulation with Pt electrodes. VIII. Electrochemically safe charge injection limits with 0.2 ms pulses","volume":"37","author":"Rose","year":"1990","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"138","DOI":"10.4236\/jst.2012.23020","article-title":"Morphology and electrochemical properties of activated and sputtered iridium oxide films for functional electrostimulation","volume":"2","author":"Negi","year":"2012","journal-title":"J. Sens. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/jbm.b.31223","article-title":"Sputtered iridium oxide films for neural stimulation electrodes","volume":"89","author":"Cogan","year":"2009","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"33526","DOI":"10.1038\/srep33526","article-title":"Flexible, Neural Electrode Array Based-on Porous Graphene for Cortical Microstimulation and Sensing","volume":"6","author":"Lu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jneumeth.2004.02.019","article-title":"Over-pulsing degrades activated iridium oxide films used for intracortical neural stimulation","volume":"137","author":"Cogan","year":"2004","journal-title":"J. Neurosci. Methods"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1109\/TBME.2005.847523","article-title":"Characterization and modeling of electrodes for biomedical applications","volume":"52","author":"Franks","year":"2005","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1088\/1741-2560\/4\/2\/008","article-title":"The influence of electrolyte composition on the in vitro charge-injection limits of activated iridium oxide (AIROF) stimulation electrodes","volume":"4","author":"Cogan","year":"2007","journal-title":"J. Neural Eng."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"J7","DOI":"10.1149\/1.1842092","article-title":"Investigation of electrochemical behavior of stimulation\/sensing materials for pacemaker electrode applications. I. Pt, Ti, and TiN coated electrodes","volume":"152","author":"Norlin","year":"2005","journal-title":"J. Electrochem. Soc."},{"key":"ref_43","unstructured":"Mohtashami, S. (2011). Electrochemical Properties of Flexible Electrodes for Implanted Neuromuscular Excitation Application. [Master\u2019s Thesis, McMaster University]. Unpublished."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Jin, Y.H., Daubinger, P., Fiebich, B.L., and Stieglitz, T. (2011, January 23\u201327). A novel platinum nanowire-coated neural electrode and its electrochemical and biological characterization. Proceedings of the IEEE 24th International Conference on Micro Electro Mechanical Systems (MEMS), Cancun, Mexico.","DOI":"10.1109\/MEMSYS.2011.5734597"},{"key":"ref_45","first-page":"165","article-title":"Variation in performance of platinum electrodes with size and surface roughness","volume":"24","author":"Green","year":"2012","journal-title":"Sens. Mater."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/12\/4152\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:32:17Z","timestamp":1760196737000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/18\/12\/4152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,27]]},"references-count":45,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["s18124152"],"URL":"https:\/\/doi.org\/10.3390\/s18124152","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,27]]}}}