{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:55:52Z","timestamp":1770749752166,"version":"3.50.0"},"reference-count":43,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21874019, 21675024"],"award-info":[{"award-number":["21874019, 21675024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2017J01575, 2017J07001, 2016J01049, 2016Y9056"],"award-info":[{"award-number":["2017J01575, 2017J07001, 2016J01049, 2016Y9056"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk \u03b4-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20\u20132500 \u03bcM, the limit of detection was 1.0 \u03bcM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.<\/jats:p>","DOI":"10.3390\/s19020228","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T03:22:31Z","timestamp":1547090551000},"page":"228","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum"],"prefix":"10.3390","volume":"19","author":[{"given":"Shurong","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"given":"Xiuhua","family":"You","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"given":"Quanhui","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"given":"Guangwen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"given":"Jinghua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3233-8877","authenticated-orcid":false,"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2008.08.006","article-title":"Glutathione: Overview of its protective roles, measurement, and biosynthesis","volume":"30","author":"Forman","year":"2009","journal-title":"Mol. Asp. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.mam.2008.05.005","article-title":"Regulation of glutathione synthesis","volume":"30","author":"Lu","year":"2009","journal-title":"Mol. Asp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.bbadis.2011.10.003","article-title":"Elevation of glutathione as a therapeutic strategy in Alzheimer disease","volume":"1822","author":"Pocernich","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1039\/c2cc17552e","article-title":"A disulfide bound-molecular beacon as a fluorescent probe for the detection of reduced glutathione and its application in cells","volume":"48","author":"Guo","year":"2012","journal-title":"Chem. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.chroma.2016.01.050","article-title":"Determination of cellular glutathione: Glutathione disulfide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection","volume":"1437","author":"Childs","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4852","DOI":"10.1021\/ac070023x","article-title":"Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption\/ionization mass spectrometry","volume":"79","author":"Huang","year":"2007","journal-title":"Anal. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"9221","DOI":"10.1021\/ac4019457","article-title":"Detection of cellular glutathione and oxidized glutathione using magnetic\u2013plasmonic nanocomposite-based \u201cTurn-Off\u201d surface enhanced raman scattering","volume":"85","author":"Saha","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/CVI.00670-13","article-title":"Development of a recombinant protein-based enzyme-linked immunosorbent assay for diagnosis of Mycoplasma bovis infection in cattle","volume":"21","author":"Wawegama","year":"2014","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.aca.2015.05.036","article-title":"Facile and controllable one-step fabrication of molecularly imprinted polymer membrane by magnetic field directed self-assembly for electrochemical sensing of glutathione","volume":"886","author":"Zhu","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yoon, S.A., Kim, W., Sharma, A., Verwilst, P., Won, M., and Lee, M.H. (2018). A fluorescent Cy7-mercaptopyridine for the selective detection of glutathione over homocysteine and cysteine. Sensors, 18.","DOI":"10.3390\/s18092897"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18928","DOI":"10.1021\/ja309079f","article-title":"BODIPY-based ratiometric fluorescent sensor for highly selective detection of glutathione over cysteine and homocysteine","volume":"134","author":"Niu","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1021\/ar300273v","article-title":"FRET-based small-molecule fluorescent probes: Rational design and bioimaging applications","volume":"46","author":"Yuan","year":"2013","journal-title":"Acc. Chem. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6143","DOI":"10.1039\/C5CS00152H","article-title":"Design strategies of fluorescent probes for selective detection among biothiols","volume":"44","author":"Niu","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1021\/ac102850y","article-title":"\u201cMolecular beacon\u201d-based fluorescent assay for selective detection of glutathione and cysteine","volume":"83","author":"Xu","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.talanta.2017.01.033","article-title":"A novel and sensitive fluorescence sensor for glutathione detection by controlling the surface passivation degree of carbon quantum dots","volume":"166","author":"Pan","year":"2017","journal-title":"Talanta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.snb.2018.01.101","article-title":"A facile fluorescence lateral flow biosensor for glutathione detection based on quantum dots-MnO2 nanocomposites","volume":"260","author":"Chen","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1007\/s12274-017-1872-0","article-title":"Self-quenched gold nanoclusters for turn-on fluorescence imaging of intracellular glutathione","volume":"11","author":"Dai","year":"2018","journal-title":"Nano Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.talanta.2017.11.057","article-title":"A label-free turn-on-off fluorescent sensor for the sensitive detection of cysteine via blocking the Ag+-enhancing glutathione-capped gold nanoclusters","volume":"179","author":"Li","year":"2018","journal-title":"Talanta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1016\/j.snb.2017.08.130","article-title":"Heterogeneous synthesis of nitrogen-doped carbon dots prepared via anhydrous citric acid and melamine for selective and sensitive turn on-off-on detection of Hg (II), glutathione and its cellular imaging","volume":"255","author":"Iqbal","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1007\/s00604-017-2187-2","article-title":"Fluorometric determination and imaging of glutathione based on a thiol-triggered inner filter effect on the fluorescence of carbon dots","volume":"184","author":"Wu","year":"2017","journal-title":"Microchim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6399","DOI":"10.1039\/C8NJ00567B","article-title":"NIR upconversion characteristics of carbon dots for selective detection of glutathione","volume":"42","author":"Gogoi","year":"2018","journal-title":"New J. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1002\/chem.201304374","article-title":"Highly luminescent N-doped carbon quantum dots as an effective multifunctional fluorescence sensing platform","volume":"20","author":"Qian","year":"2014","journal-title":"Chem. Eur. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6081","DOI":"10.1007\/s00216-017-0541-1","article-title":"Switch-on fluorescent strategy based on crystal violet-functionalized CdTe quantum dots for detecting L-cysteine and glutathione in water and urine","volume":"409","author":"Sheng","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3945","DOI":"10.1021\/la204380a","article-title":"Gold nanocluster-based fluorescent probes for near-infrared and turn-on sensing of glutathione in living cells","volume":"28","author":"Tian","year":"2012","journal-title":"Langmuir"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1039\/C5TB01839K","article-title":"A facile and one-step ethanol-thermal synthesis of MoS2 quantum dots for two-photon fluorescence imaging","volume":"4","author":"Gu","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8335","DOI":"10.1021\/acs.analchem.7b01558","article-title":"MoS2 quantum dots as new electrochemiluminescence emitters for ultrasensitive bioanalysis of lipopolysaccharide","volume":"89","author":"Zhao","year":"2017","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/j.carbon.2017.07.086","article-title":"Oxygen-incorporated few-layer MoS2 vertically aligned on three-dimensional graphene matrix for enhanced catalytic performances in quantum dot sensitized solar cells","volume":"123","author":"Wu","year":"2017","journal-title":"Carbon"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1021\/ac00146a015","article-title":"Calculation for fluorescence modulation by absorbing species and its application to measurements using optical fibers","volume":"59","author":"Yuan","year":"1987","journal-title":"Anal. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7294","DOI":"10.1021\/ac051010r","article-title":"Copper ion-selective fluorescent sensor based on the inner filter effect using a spiropyran derivative","volume":"77","author":"Shao","year":"2005","journal-title":"Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12206","DOI":"10.1021\/ac503215z","article-title":"Single-layer MnO2 nanosheets suppressed fluorescence of 7-hydroxycoumarin: Mechanistic study and application for sensitive sensing of ascorbic acid in vivo","volume":"86","author":"Zhai","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.bios.2016.11.046","article-title":"MnO2 nanosheets as an artificial enzyme to mimic oxidase for rapid and sensitive detection of glutathione","volume":"90","author":"Liu","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15938","DOI":"10.1021\/ja804503f","article-title":"Room-temperature synthesis of manganese oxide monosheets","volume":"130","author":"Kai","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6152","DOI":"10.1021\/jacs.5b02780","article-title":"Protein induces layer-by-layer exfoliation of transition metal dichalcogenides","volume":"137","author":"Guan","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7463","DOI":"10.1021\/ac5012014","article-title":"Molybdenum disulfide quantum dots as a photoluminescence sensing platform for 2,4,6-trinitrophenol detection","volume":"86","author":"Wang","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3426","DOI":"10.1021\/ac500336f","article-title":"Turn-on fluorescence sensor for intracellular imaging of glutathione using g-C3N4 nanosheet\u2013MnO2 sandwich nanocomposite","volume":"86","author":"Zhang","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"20168","DOI":"10.1021\/ja2100774","article-title":"Intracellular glutathione detection using MnO2-nanosheet-modified upconversion nanoparticles","volume":"133","author":"Deng","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"13414","DOI":"10.1039\/C6NR03129C","article-title":"A magnetic\/fluorometric bimodal sensor based on carbon dots MnO2 platform for glutathione detection","volume":"8","author":"Xu","year":"2016","journal-title":"Nanoscale"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1039\/C5AY03108G","article-title":"Signal-on fluorescent sensor based on GQDs\u2013MnO2 composite for glutathione","volume":"8","author":"Liu","year":"2016","journal-title":"Anal. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aca.2017.02.025","article-title":"Colorimetric detection of glutathione in cells based on peroxidase-like activity of gold nanoclusters: A promising powerful tool for identifying cancer cells","volume":"967","author":"Feng","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9710","DOI":"10.1021\/ac901935a","article-title":"Graphene oxide amplified electrogenerated chemiluminescence of quantum dots and its selective sensing for glutathione from thiol-containing compounds","volume":"81","author":"Wang","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1093\/clinchem\/41.10.1509","article-title":"Blood and plasma glutathione measured in healthy subjects by HPLC: Relation to sex, aging, biological variables, and life habits","volume":"41","author":"Michelet","year":"1995","journal-title":"Clin. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7674","DOI":"10.1021\/ja401360a","article-title":"Reversible near-infrared fluorescent probe introducing tellurium to mimetic glutathione peroxidase for monitoring the redox cycles between peroxynitrite and glutathione in vivo","volume":"135","author":"Yu","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1039\/C3CC47755J","article-title":"An MnO2 nanosheet as a label-free nanoplatform for homogeneous biosensing","volume":"50","author":"Yuan","year":"2014","journal-title":"Chem. Commun."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/2\/228\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:24:39Z","timestamp":1760185479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/2\/228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,9]]},"references-count":43,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,1]]}},"alternative-id":["s19020228"],"URL":"https:\/\/doi.org\/10.3390\/s19020228","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,9]]}}}