{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T17:30:15Z","timestamp":1772559015401,"version":"3.50.1"},"reference-count":29,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["737089"],"award-info":[{"award-number":["737089"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Seventh Framework Programme","award":["336917"],"award-info":[{"award-number":["336917"]}]},{"DOI":"10.13039\/501100010570","name":"Nieders\u00e4chsisches Ministerium f\u00fcr Wissenschaft und Kultur","doi-asserted-by":"publisher","award":["LENA-Optosense Quantum- and Nanometrology (QUANOMET)"],"award-info":[{"award-number":["LENA-Optosense Quantum- and Nanometrology (QUANOMET)"]}],"id":[{"id":"10.13039\/501100010570","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.<\/jats:p>","DOI":"10.3390\/s19051234","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T03:49:31Z","timestamp":1552362571000},"page":"1234","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-4287","authenticated-orcid":false,"given":"Gregor","family":"Scholz","sequence":"first","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4945-6178","authenticated-orcid":false,"given":"Shinta","family":"Mariana","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"given":"Agus Budi","family":"Dharmawan","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Faculty of Information Technology, Universitas Tarumanagara, Jakarta 11440, Indonesia"}]},{"given":"Iqbal","family":"Syamsu","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Research Center for Electronics and Telecommunication, Indonesian Institute of Sciences (LIPI), Bandung 40135, Indonesia"}]},{"given":"Philipp","family":"H\u00f6rmann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"given":"Carsten","family":"Reuse","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"given":"Jana","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"given":"Karsten","family":"Hiller","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7055-5499","authenticated-orcid":false,"given":"Joan Daniel","family":"Prades","sequence":"additional","affiliation":[{"name":"MIND-IN<sup>2<\/sup>UB, Department of Electronic and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4522-3625","authenticated-orcid":false,"given":"Hutomo Suryo","family":"Wasisto","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]},{"given":"Andreas","family":"Waag","sequence":"additional","affiliation":[{"name":"Institute of Semiconductor Technology (IHT), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"},{"name":"Laboratory for Emerging Nanometrology (LENA), Technische Universit\u00e4t Braunschweig, 38106 Braunschweig, Germany"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1039\/C7LC00942A","article-title":"Microsensor systems for cell metabolism\u2014From 2D culture to organ-on-chip","volume":"18","author":"Kieninger","year":"2018","journal-title":"Lab 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