{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:59:54Z","timestamp":1777510794320,"version":"3.51.4"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16K14969"],"award-info":[{"award-number":["16K14969"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Wireless biosensor systems were developed in our lab for monitoring blood glucose concentrations in fish as an indicator of fish stress. However, uniform immobilization of the enzyme on the surface of the electrode is difficult, so the sensor response is typically reduced at a range of high glucose concentrations during the stress monitoring. In this study, we attempted to enhance sensor response by using a self-assembled monolayer-immobilized enzyme. Glucose oxidase was immobilized on a working electrode modified with a self-assembled monolayer. The proposed biosensor showed a good correlation between the output current and the glucose concentration range of 10\u20133500 mg dL\u22121 under an optimized working condition. The dynamic measurement range of this newly developed sensor is significantly improved, especially over a high concentration range, which helps the sensor to achieve better performance in dramatic changes in the stress response of fish. In addition, we used biological samples from test fish and obtained a good correlation coefficient between the sensor output current and the glucose concentration using a conventional method. The proposed wireless biosensor system was also applied to monitor fish stress responses in real time through different stressors and to obtain some precise data that reflect real fish stress responses.<\/jats:p>","DOI":"10.3390\/s19071518","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T03:38:52Z","timestamp":1553830732000},"page":"1518","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer"],"prefix":"10.3390","volume":"19","author":[{"given":"Haiyun","family":"Wu","sequence":"first","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzu","family":"Fujii","sequence":"additional","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiki","family":"Nakano","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science, Tohoku University, Sendai-shi 9808572, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Arimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Murata","sequence":"additional","affiliation":[{"name":"Hokkaido Industrial Technology Center, Hakodate-shi 0410801, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruto","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Hokkaido Industrial Technology Center, Hakodate-shi 0410801, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasutoshi","family":"Yoshiura","sequence":"additional","affiliation":[{"name":"National Research Institute of Fisheries and Environment of Inland Sea, Japan Fisheries Research and Education Agency, Takamatsu-shi 761-0111, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Ohnuki","sequence":"additional","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Endo","sequence":"additional","affiliation":[{"name":"Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 1088477, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0959-8030(91)90019-G","article-title":"Physiological changes in fish from stress in aquaculture with emphasis on the response and effect of corticosteroids","volume":"1","author":"Barton","year":"1991","journal-title":"Annu. 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