{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:23:02Z","timestamp":1777432982423,"version":"3.51.4"},"reference-count":35,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21804045, 21275059 and 21575044"],"award-info":[{"award-number":["21804045, 21275059 and 21575044"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2018J05020, and 2016J01062"],"award-info":[{"award-number":["2018J05020, and 2016J01062"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016808","name":"Natural Science Foundation of Xiamen City","doi-asserted-by":"publisher","award":["3502Z20183027, and 3502Z20183040"],"award-info":[{"award-number":["3502Z20183027, and 3502Z20183040"]}],"id":[{"id":"10.13039\/100016808","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University","award":["ZQN-PY612"],"award-info":[{"award-number":["ZQN-PY612"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2\u2032-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H2O2 system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg\/mL to 500 ng\/mL, with the limit of detection (LOD) of 2.0 pg\/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications.<\/jats:p>","DOI":"10.3390\/s19102224","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T10:42:33Z","timestamp":1557830553000},"page":"2224","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4761-6844","authenticated-orcid":false,"given":"Xuexia","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiyun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honggui","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Marine Engineering, Jimei University, Xiamen 361021 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cui","family":"Wang","sequence":"additional","affiliation":[{"name":"Applied and Environment Microbiology, Department of Biology, Georgie State University, Atlanta, GA 30303, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlong","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4081-9179","authenticated-orcid":false,"given":"Ranjith Kumar","family":"Kankala","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5542-6119","authenticated-orcid":false,"given":"Shu-Feng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering &amp; Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.trac.2018.05.012","article-title":"Nanozyme: An emerging alternative to natural enzyme for biosensing and immunoassay","volume":"105","author":"Wang","year":"2018","journal-title":"Trac-Trend Anal. 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