{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:54:10Z","timestamp":1760550850203,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T00:00:00Z","timestamp":1559001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701274"],"award-info":[{"award-number":["31701274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["201612010"],"award-info":[{"award-number":["201612010"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Aluminum is the most abundant metallic element in the Earth\u2019s crust and acts as a non-essential element for biological species. The accumulation of excessive amounts of aluminum can be harmful to biological species. Thus, the development of convenient and selective tools for the aluminum detection is necessary. In this work, a highly selective aluminum ion fluorescent probe N\u2019-(2,5-dihydroxybenzylidene)acetohydrazide (Al-II) has been successfully synthesized and systemically characterized. The fluorescence intensity of this probe shows a significant enhancement in the presence of Al3+, which is subject to the strong quench effects caused by Cu2+ and Fe3+. The binding ratio of probe-Al3+ was determined from the Job\u2019s plot to be 1:1. Moreover, the probe was demonstrated to be effective for in vivo imaging of the intracellular aluminum ion in both living Drosophila S2 cells and Malpighian tubules.<\/jats:p>","DOI":"10.3390\/s19112423","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T11:18:09Z","timestamp":1559042289000},"page":"2423","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Highly Selective Turn-on Fluorescent Probe for the Detection of Aluminum and Its Application to Bio-Imaging"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2009-9074","authenticated-orcid":false,"given":"Liguo","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongzhao","family":"Ran","sequence":"additional","affiliation":[{"name":"Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8466-0043","authenticated-orcid":false,"given":"Ying","family":"Su","sequence":"additional","affiliation":[{"name":"Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China"},{"name":"Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10937400701597766","article-title":"Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide","volume":"10","author":"Krewski","year":"2007","journal-title":"J. Toxicol. Environ. Health B Crit. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1007\/s11033-011-0954-4","article-title":"Molecular and physiological strategies to increase aluminum resistance in plants","volume":"39","author":"Rengel","year":"2012","journal-title":"Mol. Biol. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"913","DOI":"10.3233\/JAD-150595","article-title":"A History of In Vivo Neutron Activation Analysis in Measurement of Aluminum in Human Subjects","volume":"50","author":"Mohseni","year":"2016","journal-title":"J. Alzheimers. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1126\/science.7367858","article-title":"Alzheimer\u2019s disease: X-ray spectrometric evidence of aluminum accumulation in neurofibrillary tangle-bearing neurons","volume":"208","author":"Perl","year":"1980","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1021\/es030713s","article-title":"Effective recovery of harmful metal ions from squid wastes using subcritical and supercritical water treatments","volume":"39","author":"Tavakoli","year":"2005","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1039\/c3an00198a","article-title":"Ratiometric fluorescence imaging of cellular hypochlorous acid based on heptamethine cyanine dyes","volume":"138","author":"Lou","year":"2013","journal-title":"Analyst"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/S0891-5849(99)00065-9","article-title":"Protein carbonyl formation on mucosal proteins in vitro and in dextran sulfate-induced colitis","volume":"27","author":"Blackburn","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1021\/ac00131a019","article-title":"Fluorescence properties of metal complexes of 8-Hydroxyquinoline-5-sulfonic acid and chromatographic applications","volume":"59","author":"Soroka","year":"1987","journal-title":"Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0010-8545(00)00246-0","article-title":"Design principles of fluorescent molecular sensors for cation recognition","volume":"205","author":"Valeur","year":"2000","journal-title":"Coord. Chem. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jphotochem.2014.02.010","article-title":"Alizarin Complexone as a highly selective ratiometric fluorescent probe for Al3+ detection in semi-aqueous solution","volume":"281","author":"Wang","year":"2014","journal-title":"J. Photoch. Photobio. A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/bio.3041","article-title":"A novel fluorescent probe based on rhodamine hydrazone derivatives bearing a thiophene group for Al3+","volume":"31","author":"Li","year":"2016","journal-title":"Luminescence"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1002\/open.201402169","article-title":"A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging","volume":"4","author":"Jiang","year":"2015","journal-title":"ChemistryOpen"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1039\/C3AN02247A","article-title":"A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo","volume":"139","author":"Xiao","year":"2014","journal-title":"Analyst"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1007\/s10895-017-2056-8","article-title":"A Highly Selective and Sensitive Fluorescent Turn-on Probe for Al3+ Based on Naphthalimide Schiff Base","volume":"27","author":"Peng","year":"2017","journal-title":"J. Fluoresc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.1039\/C8AN01801D","article-title":"Fluorescent organic nanoparticles (FONs) as convenient probes for metal ion detection in aqueous medium","volume":"144","author":"Ahmed","year":"2019","journal-title":"Analyst"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1016\/j.tetlet.2012.08.058","article-title":"A turn-on Schiff base fluorescence sensor for zinc ion","volume":"53","author":"Hsieh","year":"2012","journal-title":"Tetrahedron Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1016\/j.tetlet.2013.04.115","article-title":"A turn-on chemosensor based on naphthol\u2013triazole for Al(III) and its application in bioimaging","volume":"54","author":"Jia","year":"2013","journal-title":"Tetrahedron Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1038\/cr.2013.10","article-title":"Decoding the phosphorylation code in Hedgehog signal transduction","volume":"23","author":"Chen","year":"2013","journal-title":"Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4477","DOI":"10.1016\/j.bmcl.2016.07.073","article-title":"Highly sensitive and selective detection of Al(III) ions in aqueous buffered solution with fluorescent peptide-based sensor","volume":"26","author":"In","year":"2016","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.snb.2013.06.039","article-title":"Efficient FRET-based colorimetric and ratiometric fluorescent chemosensor for Al3+ in living cells","volume":"186","author":"Li","year":"2013","journal-title":"Sens. Actuators, B"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.saa.2018.03.053","article-title":"Turn on macrocyclic chemosensor for Al(3+) ion with facile synthesis and application in live cell imaging","volume":"199","author":"Ezhumalai","year":"2018","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.saa.2019.01.031","article-title":"A selective diaminomaleonitrile-based dual channel emissive probe for Al3+ and its application in living cell imaging","volume":"212","author":"Li","year":"2019","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.saa.2017.09.007","article-title":"A highly selective and sensitive turn-on probe for aluminum(III) based on quinoline Schiff\u2019s base and its cell imaging","volume":"190","author":"Zhou","year":"2018","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jphotochem.2017.08.055","article-title":"A water soluble, highly sensitive and selective fluorescent probe for Al3+ ions and its application in live cell imaging","volume":"348","author":"Gupta","year":"2017","journal-title":"J. Photochem. Photobiol. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4721","DOI":"10.1039\/C7AN01478C","article-title":"New phenazine based AIE probes for selective detection of aluminium(iii) ions in presence of other trivalent metal ions in living cells","volume":"142","author":"Balamurugan","year":"2017","journal-title":"Analyst"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.snb.2014.11.144","article-title":"A turn-on fluorescence chemosensor for Al3+, F\u2212 and CN\u2212 ions, and its application in cell imaging","volume":"209","author":"Ding","year":"2015","journal-title":"Sens. Actuators, B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"34807","DOI":"10.1038\/srep34807","article-title":"The development of fluorescence turn-on probe for Al(III) sensing and live cell nucleus-nucleoli staining","volume":"6","author":"Saini","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.talanta.2018.09.104","article-title":"A novel self-assembled nanoprobe for the detection of aluminum ions in real water samples and living cells","volume":"194","author":"Liu","year":"2019","journal-title":"Talanta"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Li, C.L., Lu, P.H., Fu, S.F., and Wu, A.T. (2019). A Highly Selective and Sensitive Fluorescent Chemosensor for Detecting Al3+ Ion in Aqueous Solution and Plant Systems. Sensors, 19.","DOI":"10.3390\/s19030623"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.saa.2018.08.063","article-title":"A high performance 2-hydroxynaphthalene Schiff base fluorescent chemosensor for Al3+ and its applications in imaging of living cells and zebrafish in vivo","volume":"207","author":"Tian","year":"2019","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.mrfmmm.2007.01.013","article-title":"Zinc fluxes and zinc transporter genes in chronic diseases","volume":"622","author":"Devirgiliis","year":"2007","journal-title":"Mutat. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.abb.2016.04.016","article-title":"What can flies tell us about zinc homeostasis?","volume":"611","author":"Xiao","year":"2016","journal-title":"Arch. Biochem. Biophys."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/11\/2423\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:53:56Z","timestamp":1760187236000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/11\/2423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,28]]},"references-count":32,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["s19112423"],"URL":"https:\/\/doi.org\/10.3390\/s19112423","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2019,5,28]]}}}