{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T01:56:56Z","timestamp":1773021416324,"version":"3.50.1"},"reference-count":76,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T00:00:00Z","timestamp":1559520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Surface-plasmon-resonance (SPR) is a quantum-electromagnetic phenomenon arising from the interaction of light with free electrons at a metal-dielectric interface. At a specific angle\/wavelength of light, the photon\u2019s energy is transferred to excite the oscillation of the free electrons on the surface. A change in the refractive-index (RI) may occur, which is influenced by the analyte concentration in the medium in close contact with the metal surface. SPR has been widely used for the detection of gaseous, liquid, or solid samples. In this study, a functionalized specific SPR chip was designed and used in a novel point-of-care SPR module (PhotonicSys SPR H5) for the detection of the stroke biomarkers NT-proBNP and S100\u03b2. These biomarkers have proven to be good for stroke diagnosis, with sensitivity and specificity of &gt;85%. Specific detection was done by binding a biomolecular-recognizing antibody onto the Au SPR-chip. Detection was tested in water and plasma samples. NT-proBNP and S100\u03b2 were detected in a range of concentrations for stroke, from 0.1 ng\/mL to 10 ng\/mL. The RI of the blank plasma samples was 1.362412, and the lowest concentration tested for both biomarkers showed a prominent shift in the RI signal (0.25 ng\/mL NT-proBNP (1.364215) and S100\u03b2 (1.364024)). The sensor demonstrated a clinically relevant limit-of-detection of less than ng\/mL.<\/jats:p>","DOI":"10.3390\/s19112533","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T11:31:01Z","timestamp":1559561461000},"page":"2533","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Point-of-Care Surface Plasmon Resonance Biosensor for Stroke Biomarkers NT-proBNP and S100\u03b2 Using a Functionalized Gold Chip with Specific Antibody"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4119-6284","authenticated-orcid":false,"given":"Dorin","family":"Harpaz","sequence":"first","affiliation":[{"name":"School of Material Science &amp; Engineering, Nanyang Technology University, 50 Nanyang Avenue, Singapore 639798, Singapore"},{"name":"Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel"},{"name":"Institute for Sports Research (ISR), Nanyang Technology University and Loughborough University, Nanyang Avenue, Singapore 639798, Singapore"}]},{"given":"Brescia","family":"Koh","sequence":"additional","affiliation":[{"name":"School of Material Science &amp; Engineering, Nanyang Technology University, 50 Nanyang Avenue, Singapore 639798, Singapore"}]},{"given":"Robert S.","family":"Marks","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel"},{"name":"The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel"},{"name":"The Ilse Katz Centre for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel"}]},{"given":"Raymond C.S.","family":"Seet","sequence":"additional","affiliation":[{"name":"Division of Neurology, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 10 Medical Dr, Singapore 117597, Singapore"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1474-5412","authenticated-orcid":false,"given":"Ibrahim","family":"Abdulhalim","sequence":"additional","affiliation":[{"name":"School of Material Science &amp; Engineering, Nanyang Technology University, 50 Nanyang Avenue, Singapore 639798, Singapore"},{"name":"The Ilse Katz Centre for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel"},{"name":"Department of Electro optics and Photonics Engineering, Ben Gurion University of the Negev, Beer-Sheva 84105, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3546-7180","authenticated-orcid":false,"given":"Alfred I.Y.","family":"Tok","sequence":"additional","affiliation":[{"name":"School of Material Science &amp; Engineering, Nanyang Technology University, 50 Nanyang Avenue, Singapore 639798, Singapore"},{"name":"Institute for Sports Research (ISR), Nanyang Technology University and Loughborough University, Nanyang Avenue, Singapore 639798, Singapore"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Harpaz, D., Eltzov, E., Seet, R.C.S., Marks, R.S., and Tok, A.I.Y. 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