{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T22:05:32Z","timestamp":1784239532014,"version":"3.55.0"},"reference-count":32,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T00:00:00Z","timestamp":1562544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 106-2221-E-224-047"],"award-info":[{"award-number":["MOST 106-2221-E-224-047"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 107-2221-E-224-030"],"award-info":[{"award-number":["MOST 107-2221-E-224-030"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Two types of urea biosensors were integrated with a wireless measurement system and microfluidic measurement system. The two biosensors used were (i) a magnetic beads (MBs)-urease\/graphene oxide (GO)\/titanium dioxide (TiO2)-based biosensor and (ii) an MBs-urease\/GO\/ nickel oxide (NiO)-based biosensor, respectively. The wireless measurement system work exhibited the feasibility for the remote detection of urea, but it will require refinement and modification to improve stability and precision. The microchannel fluidic system showed the measurement reliability. The sensing properties of urea biosensors at different flow rates were investigated. From the measurement results, the decay of average sensitivity may be attributed to the induced vortex-induced vibrations (VIV) at the high flow rate. In the aspect of wireless monitoring, the average sensitivity of the urea biosensor based on MBs-urease\/GO\/NiO was 4.780 mV\/(mg\/dl) and with the linearity of 0.938. In the aspect of measurement under dynamic conditions, the average sensitivity of the urea biosensor based on MBs-urease\/GO\/NiO were 5.582 mV\/(mg\/dl) and with the linearity of 0.959. Both measurements performed NiO was better than TiO2 according to the comparisons.<\/jats:p>","DOI":"10.3390\/s19133004","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T11:02:37Z","timestamp":1562583757000},"page":"3004","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System &amp; Microfluidic Measurement System"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8829-3404","authenticated-orcid":false,"given":"Jung-Chuan","family":"Chou","sequence":"first","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cian-Yi","family":"Wu","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2791-9642","authenticated-orcid":false,"given":"Po-Yu","family":"Kuo","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Hsien","family":"Lai","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsun","family":"Nien","sequence":"additional","affiliation":[{"name":"Graduate School of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Xiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsu-Yang","family":"Lai","sequence":"additional","affiliation":[{"name":"Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,8]]},"reference":[{"key":"ref_1","unstructured":"(2018, June 30). 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