{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:02:05Z","timestamp":1773925325177,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T00:00:00Z","timestamp":1567641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Special Fund for Agro-scientific Research in the Public Interest","award":["201403071"],"award-info":[{"award-number":["201403071"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 21305158"],"award-info":[{"award-number":["No. 21305158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Agricultural Science and Technology Innovation Program","award":["ASTIP-IAS12"],"award-info":[{"award-number":["ASTIP-IAS12"]}]},{"name":"Modern Agro-Industry Technology Research System of the PR China","award":["CARS-36"],"award-info":[{"award-number":["CARS-36"]}]},{"name":"Project of risk assessment on raw milk","award":["GJFP2018008"],"award-info":[{"award-number":["GJFP2018008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, a rapid and sensitive fluorescent aptasensor for the detection of aflatoxin M1 (AFM1) in milk powder was developed. Graphene oxide (GO) was employed to quench the fluorescence of a carboxyfluorescein-labelled aptamer and protect the aptamer from nuclease cleavage. Upon the addition of AFM1, the formation of an AFM1\/aptamer complex resulted in the aptamer detaching from the surface of GO, followed by the aptamer cleavage by DNase I and the release of the target AFM1 for a new cycle, which led to great signal amplification and high sensitivity. Under optimized conditions, the GO-based detection of the aptasensor exhibited a linear response to AFM1 levels in a dynamic range from 0.2 to 10 \u03bcg\/kg, with a limit of detection (LOD) of 0.05 \u03bcg\/kg. Moreover, the developed aptasensor showed a high specificity towards AFM1 without interference from other mycotoxins. In addition, the technique was successfully applied for the detection of AFM1 in infant milk powder samples. The aptasensor proposed here offers a promising technology for food safety monitoring and can be extended to various targets.<\/jats:p>","DOI":"10.3390\/s19183840","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T02:59:22Z","timestamp":1567738762000},"page":"3840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["A Novel Graphene Oxide-Based Aptasensor for Amplified Fluorescent Detection of Aflatoxin M1 in Milk Powder"],"prefix":"10.3390","volume":"19","author":[{"given":"Xiaodong","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"Chimie g\u00e9n\u00e9rale et organique, Gembloux Agro-Bio Tech, Universit\u00e9 de Li\u00e8ge, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"},{"name":"Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Saive","sequence":"additional","affiliation":[{"name":"Chimie g\u00e9n\u00e9rale et organique, Gembloux Agro-Bio Tech, Universit\u00e9 de Li\u00e8ge, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2027-3896","authenticated-orcid":false,"given":"Marie-Laure","family":"Fauconnier","sequence":"additional","affiliation":[{"name":"Chimie g\u00e9n\u00e9rale et organique, Gembloux Agro-Bio Tech, Universit\u00e9 de Li\u00e8ge, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality &amp; Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"},{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193 Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,5]]},"reference":[{"key":"ref_1","unstructured":"International Agency for Research on Cancer (1993). 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