{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:05:03Z","timestamp":1776445503206,"version":"3.51.2"},"reference-count":40,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2019,9,14]],"date-time":"2019-09-14T00:00:00Z","timestamp":1568419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Previous works showed that spatially resolved Raman spectra of cytoplasm and nucleus region of single cells exposed to X-rays evidence different features. The present work aims to introduce a new approach to profit from these differences to deeper investigate X-ray irradiation effects on single SH-SY5Y human neuroblastoma cells. For this aim, Raman micro-spectroscopy was performed in vitro on single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were selectively acquired. The examination by interval Principal Component Analysis (i-PCA) of the difference spectra obtained by subtracting each cytoplasm-related spectrum from the corresponding one detected at the nucleus enabled us to reveal the subtle modifications of Raman features specific of different spatial cell regions. They were discussed in terms of effects induced by X-ray irradiation on DNA\/RNA, lipids, and proteins. The proposed approach enabled us to evidence some features not outlined in previous investigations.<\/jats:p>","DOI":"10.3390\/s19183971","type":"journal-article","created":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T03:17:57Z","timestamp":1568603877000},"page":"3971","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Multivariate Analysis of Difference Raman Spectra of the Irradiated Nucleus and Cytoplasm Region of SH-SY5Y Human Neuroblastoma Cells"],"prefix":"10.3390","volume":"19","author":[{"given":"Ines","family":"Delfino","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Ecologiche e Biologiche, Universit\u00e0 della Tuscia, 01100 Viterbo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerio","family":"Ricciardi","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, 80100 Napoli, Italy"},{"name":"Istituto Nazionale di Fisica Nucleare, sezione di Napoli, 80126 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-5040","authenticated-orcid":false,"given":"Lorenzo","family":"Manti","sequence":"additional","affiliation":[{"name":"Istituto Nazionale di Fisica Nucleare, sezione di Napoli, 80126 Napoli, Italy"},{"name":"Dipartimento di Fisica, Universit\u00e0 \u201cFederico II,\u201d 80126 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8221-7849","authenticated-orcid":false,"given":"Maria","family":"Lasalvia","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica e Sperimentale, Universit\u00e0 di Foggia, 71100 Foggia, Italy"},{"name":"Istituto Nazionale di Fisica Nucleare, sezione di Bari, 70125 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-5904","authenticated-orcid":false,"given":"Maria","family":"Lepore","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, 80100 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/nprot.2016.036","article-title":"Using Raman spectroscopy to characterize biological materials","volume":"11","author":"Butler","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41536-017-0014-3","article-title":"Raman spectroscopy and regenerative medicine: A review","volume":"2","author":"Ember","year":"2017","journal-title":"NPJ Regen. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1364\/AOP.9.000315","article-title":"Raman spectroscopy: Techniques and applications in the life sciences","volume":"9","author":"Shipp","year":"2017","journal-title":"Adv. Opt. 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