{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:39:08Z","timestamp":1774474748634,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T00:00:00Z","timestamp":1569024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21804114"],"award-info":[{"award-number":["21804114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Natural Science Foundation of Hunan Province","award":["2018JJ3500"],"award-info":[{"award-number":["2018JJ3500"]}]},{"name":"the Research Foundation for PhD of Xiangtan University","award":["KZ08042"],"award-info":[{"award-number":["KZ08042"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A simple, low-cost technique has been developed for the rapid fabrication of single-layered paper-based microfluidic devices (\u03bcPADs). This technique, for the first time, made use of the deposition of patterned adhesive tape into the filter paper to construct hydrophobic barriers, with the help of toluene. Unlike other reported multi-layered \u03bcPADs that merely made use of adhesive tape as a separate layer for sealing or fluid flow controlling, the patterned adhesive tape was simultaneously dissolved and penetrated into the filter paper, which resulted in the successful transfer of the pattern from the tape to the filter paper. To demonstrate the effectiveness of this approach, nitrite and glucose were individually measured; detection limits as low as 0.015 \u00b1 0.004 mM and 0.022 \u00b1 0.006 mM were reported for nitrite and glucose, respectively. Multiplexed analysis of both analytes was also carried out with respective detection limits of 0.048 \u00b1 0.005 mM and 0.025 \u00b1 0.006 mM for nitrite and glucose. The application of the method was demonstrated by measuring nitrite and glucose in spiked artificial urine samples and satisfied recovery results were obtained.<\/jats:p>","DOI":"10.3390\/s19194082","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T03:26:32Z","timestamp":1569209192000},"page":"4082","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["New Single-Layered Paper-Based Microfluidic Devices for the Analysis of Nitrite and Glucose Built via Deposition of Adhesive Tape"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3195-8049","authenticated-orcid":false,"given":"Peng","family":"Yu","sequence":"first","affiliation":[{"name":"Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, China"}]},{"given":"Muhan","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, China"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, China"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1002\/anie.200603817","article-title":"Patterned Paper as a Platform for Inexpensive, Low Volume, Portable Bioassays","volume":"46","author":"Martinez","year":"2007","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.aca.2018.12.001","article-title":"Low-cost fabrication of a paper-based microfluidic using a folded pattern paper","volume":"1053","author":"Xie","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.bios.2018.01.025","article-title":"Ultrasensitive microfluidic paper-based electrochemical\/visual biosensor based on spherical-like cerium dioxide catalyst for miR-21 detection","volume":"105","author":"Sun","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1002\/elps.201700254","article-title":"An aptamer-based paper microfluidic device for the colorimetric determination of cocaine","volume":"39","author":"Wang","year":"2018","journal-title":"Electrophoresis"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.talanta.2017.08.091","article-title":"Compact detectors made of paired LEDs for photometric and fluorometric measurements on paper","volume":"178","author":"Granica","year":"2018","journal-title":"Talanta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1016\/j.bios.2008.11.031","article-title":"Biochip functionalization using electrowetting-on-dielectric digital microfluidics for surface plasmon resonance imaging detection of DNA hybridization","volume":"24","author":"Malic","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10544-012-9716-x","article-title":"Chemiluminescence microfluidic system of gold nanoparticles enhanced luminol-silver nitrate for the determination of vitamin B12","volume":"15","author":"Kamruzzaman","year":"2013","journal-title":"Biomed. Microdevices"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2487","DOI":"10.1002\/elps.201000063","article-title":"Toner and paper-based fabrication techniques for microfluidic applications","volume":"31","author":"Coltro","year":"2010","journal-title":"Electrophoresis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s10337-013-2413-y","article-title":"Microfluidic Paper-Based Analytical Devices (\u03bcPADs) and Micro Total Analysis Systems (\u03bcTAS): Development, Applications and Future Trends","volume":"76","author":"Lisowski","year":"2013","journal-title":"Chromatographia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1002\/jms.1585","article-title":"Microfluidic chips for mass spectrometry-based proteomics","volume":"44","author":"Lee","year":"2009","journal-title":"J. Mass Spectrom."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4894","DOI":"10.1021\/acs.analchem.8b00559","article-title":"Selective distance-based K+ quantification on paper-based microfluidics","volume":"90","author":"Gerold","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1021\/ed500401d","article-title":"Cost Effective Paper-Based Colorimetric Microfluidic Devices and Mobile Phone Camera Readers for the Classroom","volume":"92","author":"Koesdjojo","year":"2015","journal-title":"J. Chem. Educ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.aca.2017.10.018","article-title":"Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device","volume":"997","author":"Rossini","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.forc.2018.03.008","article-title":"Simultaneous colorimetric detection of metallic salts contained in low explosives residue using a microfluidic paper-based analytical device (\u00b5PAD)","volume":"9","author":"Chabaud","year":"2018","journal-title":"Forensic Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1007\/s11696-017-0357-7","article-title":"Colorimetric determination of acidity constant using a paper-based microfluidic analytical device","volume":"72","author":"Hemmateenejad","year":"2018","journal-title":"Chem. Pap."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.talanta.2018.10.039","article-title":"A colorimetric assay system for dopamine using microfluidic paper-based analytical devices","volume":"194","author":"Liu","year":"2019","journal-title":"Talanta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.talanta.2017.08.082","article-title":"Portable and low-cost colorimetric office paper-based device for phenacetin detection in seized cocaine samples","volume":"176","year":"2018","journal-title":"Talanta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.talanta.2018.10.104","article-title":"Single step and mask-free 3D wax printing of microfluidic paper-based analytical devices for glucose and nitrite assays","volume":"194","author":"Chiang","year":"2019","journal-title":"Talanta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3196","DOI":"10.1109\/TED.2019.2913851","article-title":"Novel 3D Printed Microfluidic Paper-Based Analytical Device with Integrated Screen-Printed Electrodes for Automated Viscosity Measurements","volume":"66","author":"Puneeth","year":"2019","journal-title":"IEEE Trans. Electron Devices"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3616","DOI":"10.1039\/C8AY01237G","article-title":"Characterization of low adsorption filter membranes for electrophoresis and electrokinetic sample manipulations in microfluidic paper-based analytical devices","volume":"10","author":"Baker","year":"2018","journal-title":"Anal. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.aca.2015.04.014","article-title":"Development of paper-based microfluidic analytical device for iron assay using photomask printed with 3D printer for fabrication of hydrophilic and hydrophobic zones on paper by photolithography","volume":"883","author":"Asano","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1007\/s10404-014-1347-5","article-title":"One-step rapid fabrication of paper-based microfluidic devices using fluorocarbon plasma polymerization","volume":"16","author":"Kao","year":"2014","journal-title":"Microfluid. Nanofluidics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1039\/C4AN00022F","article-title":"Vapor-phase deposition of polymers as a simple and versatile technique to generate paper-based microfluidic platforms for bioassay applications","volume":"139","author":"Demirel","year":"2014","journal-title":"Analyst"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7311","DOI":"10.1039\/C5AY00466G","article-title":"Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms","volume":"7","author":"Cardoso","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.talanta.2015.10.013","article-title":"Paper-based analytical device for instrumental-free detection of thiocyanate in saliva as a biomarker of tobacco smoke exposure","volume":"147","author":"Lavilla","year":"2016","journal-title":"Talanta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.talanta.2013.05.037","article-title":"Fabrication of paper-based devices by lacquer spraying method for the determination of nickel (II) ion in waste water","volume":"114","author":"Nurak","year":"2013","journal-title":"Talanta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19606","DOI":"10.1073\/pnas.0810903105","article-title":"Three-dimensional microfluidic devices fabricated in layered paper and tape","volume":"105","author":"Martinez","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3284","DOI":"10.1016\/j.bios.2010.12.044","article-title":"A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for uric acid determination","volume":"26","author":"Yu","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4474","DOI":"10.1021\/ac300309c","article-title":"Microfluidic paper-based analytical device for particulate metals","volume":"84","author":"Mentele","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4824","DOI":"10.1038\/s41598-019-41208-y","article-title":"Reconfigurable Acrylic-tape Hybrid Microfluidics","volume":"9","author":"Ren","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.redox.2012.11.005","article-title":"Nitrite: A physiological store of nitric oxide and modulator of mitochondrial function","volume":"1","author":"Shiva","year":"2013","journal-title":"Redox Boil."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/j.apsusc.2018.01.156","article-title":"Rapid and simple preparation of rhodamine 6G loaded HY zeolite for highly selective nitrite detection","volume":"440","author":"Viboonratanasri","year":"2018","journal-title":"Appl. Surf. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1136\/adc.62.2.138","article-title":"Urinary nitrite in symptomatic and asymptomatic urinary infection","volume":"62","author":"Powell","year":"1987","journal-title":"Arch. Dis. Child."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.snb.2018.09.076","article-title":"Co-MOF nanosheet array: A high-performance electrochemical sensor for non-enzymatic glucose detection","volume":"278","author":"Li","year":"2019","journal-title":"Sens. Actuators B Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.bios.2018.03.025","article-title":"Nanostructured silver fabric as a free-standing NanoZyme for colorimetric detection of glucose in urine","volume":"110","author":"Karim","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3390\/s30800276","article-title":"Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay","volume":"3","author":"Sun","year":"2003","journal-title":"Sensors"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4749","DOI":"10.1039\/C6AN00430J","article-title":"Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices","volume":"141","author":"Gabriel","year":"2016","journal-title":"Analyst"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10270","DOI":"10.1021\/ac402055n","article-title":"Laminated Paper-Based Analytical Devices (LPAD) with Origami-Enabled Chemiluminescence Immunoassay for Cotinine Detection in Mouse Serum","volume":"85","author":"Liu","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.bios.2011.10.019","article-title":"Paper-based chemiluminescence ELISA: Lab-on-paper based on chitosan modified paper device and wax-screen-printing","volume":"31","author":"Wang","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"17690","DOI":"10.1039\/C4RA16296J","article-title":"Antibacterial wound dressing: Plasma treatment effect on chitosan impregnation and in situ synthesis of silver chloride on cellulose surface","volume":"5","year":"2015","journal-title":"RSC Adv."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0022-0248(01)01547-0","article-title":"Laser-probe-based investigation of the evolution of particle size distributions of calcium oxalate particles formed in artificial urines","volume":"233","author":"Laube","year":"2001","journal-title":"J. Cryst. Growth"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7858","DOI":"10.1021\/ac501786u","article-title":"IUPAC-consistent approach to the limit of detection in partial least-squares calibration","volume":"86","author":"Allegrini","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1039\/C8AN02085J","article-title":"Electrochemical-digital immunosensor with enhanced sensitivity for detecting human salivary glucocorticoid hormone","volume":"144","author":"Khan","year":"2019","journal-title":"Analyst"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0169-7439(01)00155-1","article-title":"PLS-regression: A basic tool of chemometrics","volume":"58","author":"Wold","year":"2001","journal-title":"Chemometr. Intell. Lab. Syst."},{"key":"ref_45","unstructured":"Massart, D.L., Vandeginste, B.G.M., Buydens, L.M.C., De Jong, S., Lewi, P.J., and Smeyers-Verbeke, J. (1997). Handbook of Chemometrics and Qualimetrics, Elsevier."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.aca.2004.08.060","article-title":"Fabrication and characterization of copper nanoparticle thin-films and the electrocatalytic behavior","volume":"526","author":"Wang","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.talanta.2014.02.030","article-title":"Sensitive and selective determination of NO2\u2212 ion in aqueous samples using modified gold nanoparticle as a colorimetric probe","volume":"125","author":"Nam","year":"2014","journal-title":"Talanta"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6362","DOI":"10.1021\/ja901609k","article-title":"Colorimetric nitrite and nitrate detection with gold nanoparticle probes and kinetic end points","volume":"131","author":"Daniel","year":"2009","journal-title":"J. Am. Chem. Soc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s10404-015-1545-9","article-title":"Multiple semi-quantitative colorimetric assays in compact embeddable microfluidic cloth-based analytical device (\u03bcCAD) for effective point-of-care diagnostic","volume":"19","author":"Nilghaz","year":"2015","journal-title":"Microfluid. Nanofluid."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1021\/ed500644m","article-title":"Fabrication of a paper-based microfluidic device to readily determine nitrite ion concentration by simple colorimetric assay","volume":"92","author":"Wang","year":"2015","journal-title":"J. Chem. Educ."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10531","DOI":"10.1021\/ic201753v","article-title":"Two closely related iridium (III) complexes as colorimetric and fluorometric chemodosimeters for nitrite in aqueous solution operating along different modes of action","volume":"50","author":"Qinghai","year":"2011","journal-title":"Inorg. Chem."},{"key":"ref_52","unstructured":"Strasinger, S.K., and Di Lorenzo, M.S. (2014). Urinalysis and Body Fluids, FA Davis."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Yu, W., Seo, W., Tan, T., Jung, B., and Ziaie, B. (2016, January 16\u201320). A diaper-embedded disposable nitrite sensor with integrated on-board urine-activated battery for UTI screening. Proceedings of the 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Orlando, FL, USA.","DOI":"10.1109\/EMBC.2016.7590700"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"18570","DOI":"10.1038\/srep18570","article-title":"Lignocellulose-based analytical devices: Bamboo as an analytical platform for chemical detection","volume":"5","author":"Kuan","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Gabriel, E., Garcia, P., Lopes, F., and Coltro, W. (2017). Based Colorimetric Biosensor for Tear Glucose Measurements. Micromachines, 8.","DOI":"10.3390\/mi8040104"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.bios.2012.03.018","article-title":"Determination of glucose and uric acid with bienzyme colorimetry on microfluidic paper-based analysis devices","volume":"35","author":"Chen","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"37637","DOI":"10.1039\/C4RA07112C","article-title":"A handheld stamping process to fabricate microfluidic paper-based analytical devices with chemically modified surface for clinical assays","volume":"4","author":"Cardoso","year":"2014","journal-title":"Rsc Adv."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.snb.2016.01.121","article-title":"An animal cell culture monitoring system using a smartphone-mountable paper-based analytical device","volume":"229","author":"Im","year":"2016","journal-title":"Sens. Actuat. B Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1039\/C4AN01147C","article-title":"Modification of microfluidic paper-based devices with silica nanoparticles","volume":"139","author":"Evans","year":"2014","journal-title":"Analyst"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4273","DOI":"10.1021\/ac200697y","article-title":"Paper bioassay based on ceria nanoparticles as colorimetric probes","volume":"83","author":"Ornatska","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s00604-017-2575-7","article-title":"Microfluidic paper-based device for colorimetric determination of glucose based on a metal-organic framework acting as peroxidase mimetic","volume":"185","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1021\/ja010497s","article-title":"Product distribution of peroxynitrite decay as a function of pH, temperature, and concentration","volume":"124","author":"Kissner","year":"2002","journal-title":"J. Am. Chem. Soc."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/19\/4082\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:22:40Z","timestamp":1760188960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/19\/4082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,21]]},"references-count":62,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["s19194082"],"URL":"https:\/\/doi.org\/10.3390\/s19194082","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,21]]}}}