{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T20:55:34Z","timestamp":1771620934569,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI14C3319"],"award-info":[{"award-number":["HI14C3319"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A6A3A11041491"],"award-info":[{"award-number":["2018R1A6A3A11041491"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The concentration effect of dielectrophoresis (DEP) enables detection of biomolecules with high sensitivity. In this study, microstructures were patterned between the interdigitated microelectrodes (IMEs) to increase the concentration effect of DEP. The microstructures increased the electric field gradient (    \u2207  |   E 2   |     ) between the IMEs to approximately 6.61-fold higher than in the bare IMEs with a gap of 10 \u03bcm, resulting in a decreased optimal voltage to concentrate amyloid beta 42 (A\u03b242, from 0.8 Vpp to 0.5 Vpp) and tau-441 (from 0.9 Vpp to 0.6 Vpp) between the IMEs. Due to the concentration effect of DEP, the impedance change in the optimal condition was higher than the values in the reference condition at 2.64-fold in A\u03b242 detection and at 1.59-fold in tau-441 detection. This concentration effect of DEP was also verified by counting the number of gold (Au) particles which conjugated with the secondary antibody. Finally, an enhanced concentration effect in the patterned IMEs was verified by measuring the impedance change depending on the concentration of A\u03b242 and tau-441. Our results suggest that microstructures increase the concentration effect of DEP, leading to enhanced sensitivity of the IMEs.<\/jats:p>","DOI":"10.3390\/s19194152","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T03:06:51Z","timestamp":1569467211000},"page":"4152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Highly Sensitive Micropatterned Interdigitated Electrodes for Enhancing the Concentration Effect Based on Dielectrophoresis"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7070-4204","authenticated-orcid":false,"given":"Hye Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"}]},{"given":"Heeju","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"},{"name":"Department of Biomedical Engineering Korea University, Seoul 01897, Korea"}]},{"given":"David S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"}]},{"given":"Dongsung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"},{"name":"Department of Biomedical Engineering Korea University, Seoul 01897, Korea"}]},{"given":"Jae Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"}]},{"given":"Jinsik","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Dongguk University, Seoul 04620, Korea"}]},{"given":"Dae Sung","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Korea University, Seoul 01897, Korea"}]},{"given":"Kyo Seon","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02453, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7062","DOI":"10.1039\/C5AN00780A","article-title":"Biomarker detection for disease diagnosis using cost-effective microfluidic platforms","volume":"140","author":"Sanjay","year":"2015","journal-title":"Analyst"},{"key":"ref_2","unstructured":"Mohammed, K. 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