{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:12:13Z","timestamp":1769170333645,"version":"3.49.0"},"reference-count":29,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD0901000"],"award-info":[{"award-number":["2018YFD0901000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["19DZ2284200"],"award-info":[{"award-number":["19DZ2284200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A commercially available microtiter plate reader was applied as a high-throughput counterpart of ultraviolet-visible (UV\u2013Vis) spectrophotometer to identify the producing location of extra virgin olive oils (EVOOs). Multiplicative scatter correction and the first derivative was used to denoise the UV\u2013Vis spectra and eliminate the effects of background drift. The spectra were analyzed using chemometrics methods including the principal component analysis (PCA) and the partial least squares-discriminant analysis (PLS-DA). The PLS-DA model on full spectra using 5 latent variables showed a classification accuracy of 97.92% by cross-validation. The overall results demonstrated that the use of a UV\u2013Vis spectrophotometer based on the microtiter plate reader combined with chemometrics can be applied to the quality assessment of EVOOs. It is demonstrated that the microtiter plate reader can be a high-throughput tool in the quality assessment of food ingredients.<\/jats:p>","DOI":"10.3390\/s19194169","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T03:03:15Z","timestamp":1569553395000},"page":"4169","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["High-Throughput Chemometric Quality Assessment of Extra Virgin Olive Oils Using a Microtiter Plate Reader"],"prefix":"10.3390","volume":"19","author":[{"given":"Huihui","family":"He","sequence":"first","affiliation":[{"name":"Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiying","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,26]]},"reference":[{"key":"ref_1","first-page":"135","article-title":"Olive Oil","volume":"Volume 13","author":"Victor","year":"2015","journal-title":"The Mediterranean Diet- an Evidence-Based Approach"},{"key":"ref_2","first-page":"133","article-title":"Effects of Extra-Virgin Olive Oil in Neurological Disorders","volume":"Volume 6","author":"Tahira","year":"2017","journal-title":"Neuroprotective Effects of Phytochemicals in Neurological Disorders"},{"key":"ref_3","first-page":"1","article-title":"Benefit of Oleuropein Aglycone for Alzheimer\u2019s Disease by Promoting Autophagy","volume":"2","author":"Ricardo","year":"2018","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_4","first-page":"299","article-title":"Neuroprotective Effects of Extra-Virgin Olive Oil and its Components in Alzheimer\u2019s Disease","volume":"Volume 14","author":"Tahira","year":"2017","journal-title":"Neuroprotective Effects of Phytochemicals in Neurological Disorders"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Angeloni, C., Malaguti, M., Barbalace, M.C., and Hrelia, S. (2017). Bioactivity of Olive Oil Phenols in Neuroprotection. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112230"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1017\/S1368980015002244","article-title":"Exclusive olive oil consumption has a protective effect on coronary artery disease; overview of the THISEAS study","volume":"19","author":"Maria","year":"2016","journal-title":"Public Health Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/biof.1318","article-title":"Nutrigenomics of extra-virgin olive oil: A review","volume":"43","author":"Marta","year":"2017","journal-title":"BioFactors"},{"key":"ref_8","first-page":"221","article-title":"Optical Nondestructive UV-Vis-NIR-MIR Spectroscopic Tools and Chemometrics in the Monitoring of Olive Oil Functional Compounds","volume":"Volume 11","author":"Apostolos","year":"2017","journal-title":"Olives and Olive Oil as Functional Foods: Bioactivity, Chemistry and Processing"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Conte, L., Bendini, A., Valli, E., Lucci, P., Moret, S., Maquet, A., Lacoste, F., Brereton, P., Garc\u00eda-Gonz\u00e1lez, D.L., and Moreda, W. (2019). Olive oil quality and authenticity: A review of current EU legislation, standards, relevant methods of analyses, their drawbacks and recommendations for the future. Trends Food Sci. Technol., in press.","DOI":"10.1016\/j.tifs.2019.02.025"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.aca.2011.11.015","article-title":"Characterisation of PDO olive oil Chianti Classico by non-selective (UV\u2013visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques","volume":"712","author":"Casale","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.trac.2016.02.026","article-title":"Food authentication: Techniques, trends & emerging approaches","volume":"85","author":"Danezis","year":"2016","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1108\/03090560310465099","article-title":"Consumer evaluations of product certification, geographic association and traceability in Greece","volume":"37","author":"Efthalia","year":"2003","journal-title":"Eur. J. Mark."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3390\/foods7040044","article-title":"Authentication and quantitation of fraud in extra virgin olive oils based on HPLC-UV fingerprinting and multivariate calibration","volume":"7","author":"Mireia","year":"2018","journal-title":"Foods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.trac.2004.07.016","article-title":"Vanguard-rearguard analytical strategies","volume":"24","year":"2005","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1016\/j.talanta.2015.07.067","article-title":"Artificial nose, NIR and UV\u2013visible spectroscopy for the characterisation of the PDO Chianti Classico olive oil","volume":"144","author":"Forina","year":"2015","journal-title":"Talanta"},{"key":"ref_16","first-page":"124","article-title":"Evaluation of olive oils from the Mediterranean region by UV-Vis spectroscopy and Independent Component Analysis","volume":"273","author":"Francieli","year":"2018","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.foodchem.2016.02.038","article-title":"Olive oil sensory defects classification with data fusion of instrumental techniques and multivariate analysis (PLS-DA)","volume":"203","author":"Eva","year":"2016","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1366\/000370208786049097","article-title":"Loopy MSC: A simple way to improve multiplicative scatter correction","volume":"62","author":"Willem","year":"2008","journal-title":"Appl. Spectrosc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/nmeth.4551","article-title":"Points of significance: Machine learning: Supervised methods","volume":"15","author":"Danilo","year":"2018","journal-title":"Nat. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1002\/jsfa.1426","article-title":"Classification of monovarietal Italian olive oils by unsupervised (PCA) and supervised (LDA) chemometrics","volume":"83","author":"Lucia","year":"2003","journal-title":"J. Sci. Food Agric."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, C., Shen, T., Liu, F., and He, Y. (2018). Identification of Coffee Varieties Using Laser-Induced Breakdown Spectroscopy and Chemometrics. Sensors, 18.","DOI":"10.3390\/s18010095"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1021\/jf305272s","article-title":"A Near-Infrared Reflectance Spectroscopy Method for Direct Analysis of Several Chemical Components and Properties of Fruit, for Example, Chinese Hawthorn","volume":"61","author":"Dong","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3790","DOI":"10.1039\/c3ay40582f","article-title":"Classification tools in chemistry. Part 1: Linear models. PLS-DA","volume":"5","author":"Davide","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.foodchem.2014.04.109","article-title":"Thermal edible oil evaluation by UV-Vis spectroscopy and chemometrics","volume":"163","author":"Rhayanna","year":"2014","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1007\/s11746-014-2515-1","article-title":"Rapid Determination of Olive Oil Chlorophylls and Carotenoids by Using Visible Spectroscopy","volume":"91","author":"Khaled","year":"2014","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_26","first-page":"110","article-title":"Principle of enzyme-linked immunosorbnent assay (ELISA) and repairing of ELISA reader","volume":"30","author":"Liu","year":"2009","journal-title":"Chin. Med. Equip. J."},{"key":"ref_27","unstructured":"(2019, September 24). Biotekinstruments. Available online: https:\/\/www.biotekinstruments.ru\/assets\/tech_resources\/LoBase_App_Note.pdf."},{"key":"ref_28","first-page":"77","article-title":"Processing of Analytic Signals","volume":"Volume 3","author":"Yi","year":"2010","journal-title":"The Basis of Chemometrics"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Li, X., Zhang, Y., Cui, H., and Shen, T. (2018, January 25\u201327). Analysis of Near Infrared Spectra of Apple Soluble Solids Content Based on BP Neural Network. Proceedings of the 37th Chinese Control Conference, Wuhan, China.","DOI":"10.23919\/ChiCC.2018.8483739"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/19\/4169\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:24:22Z","timestamp":1760189062000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/19\/4169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,26]]},"references-count":29,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["s19194169"],"URL":"https:\/\/doi.org\/10.3390\/s19194169","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,26]]}}}