{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T22:21:51Z","timestamp":1779920511189,"version":"3.53.1"},"reference-count":22,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T00:00:00Z","timestamp":1570752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/16573-5"],"award-info":[{"award-number":["2015\/16573-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Single-drop microextraction (SDME) was coupled with surface-enhanced Raman scattering (SERS) to provide sample extraction and pre-concentration for detection of analyte at low concentrations. A gold nanohole array substrate (AuNHAS), fabricated by interference lithography, was used as SERS substrate and para-mercaptobenzoic acid (p-MBA) was tested as a probe molecule, in the concentration range 10\u22128\u201310\u22124 mol L\u22121. With this approach, a limit of 10\u22127 mol L\u22121 was clearly detected. To improve the detection to lower p-MBA concentration, as 10\u22128 mol L\u22121, the SDME technique was applied. The p-MBA Raman signature was detected in two performed extractions and its new concentration was determined to be ~4.6 \u00d7 10\u22125 mol L\u22121. This work showed that coupling SDME with SERS allowed a rapid (5 min) and efficient pre-concentration (from 10\u22128 mol L\u22121 to 10\u22125 mol L\u22121), detection, and quantification of the analyte of interest, proving to be an interesting analytical tool for SERS applications.<\/jats:p>","DOI":"10.3390\/s19204394","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T10:53:03Z","timestamp":1570791183000},"page":"4394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Coupling Single-Drop Microextraction with SERS: A Demonstration Using p-MBA on Gold Nanohole Array Substrate"],"prefix":"10.3390","volume":"19","author":[{"given":"Elias B.","family":"Santos","sequence":"first","affiliation":[{"name":"LQANano, Federal University of S\u00e3o Paulo, S\u00e3o Jos\u00e9 dos Campos-SP, 12231-280, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0456-5776","authenticated-orcid":false,"given":"Chiara","family":"Valsecchi","sequence":"additional","affiliation":[{"name":"Engineering Department, Federal University of Pampa, Alegrete-RS, 97546-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaderson L. S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Engineering Department, Federal University of Pampa, Alegrete-RS, 97546-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis F.","family":"\u00c1vila","sequence":"additional","affiliation":[{"name":"Applied Optics Laboratory\u2013School of Technology, State University of Campinas, Limeira\u2013SP, 13484-350, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3062-7125","authenticated-orcid":false,"given":"Jacson W.","family":"Menezes","sequence":"additional","affiliation":[{"name":"Engineering Department, Federal University of Pampa, Alegrete-RS, 97546-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4756","DOI":"10.1002\/anie.201205748","article-title":"Surface-Enhanced Raman Spectroscopy: Concepts and Chemical Applications","volume":"53","author":"Schlucker","year":"2014","journal-title":"Angew. 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