{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T11:57:29Z","timestamp":1763035049857,"version":"build-2065373602"},"reference-count":51,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T00:00:00Z","timestamp":1572220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n (COLCIENCIAS)","doi-asserted-by":"publisher","award":["115077757186"],"award-info":[{"award-number":["115077757186"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Cutaneous leishmaniasis (CL) is a neglected tropical disease that requires novel tools for its understanding, diagnosis, and treatment follow-up. In the cases of other cutaneous pathologies, such as cancer or cutaneous ulcers due to diabetes, optical diffuse reflectance-based tools and methods are widely used for the investigation of those illnesses. These types of tools and methods offer the possibility to develop portable diagnosis and treatment follow-up systems. In this article, we propose the use of a three-layer diffuse reflectance model for the study of the formation of cutaneous ulcers caused by CL. The proposed model together with an inverse-modeling procedure were used in the evaluation of diffuse-reflectance spectral signatures acquired from cutaneous ulcers formed in the dorsal area of 21 golden hamsters inoculated with Leishmanisis braziliensis. As result, the quantification of the model\u2019s variables related to the main biological parameters of skin were obtained, such as: diameter and volumetric fraction of keratinocytes, collagen; volumetric fraction of hemoglobin, and oxygen saturation. Those parameters show statistically significant differences among the different stages of the CL ulcer formation. We found that these differences are coherent with histopathological manifestations reported in the literature for the main phases of CL formation.<\/jats:p>","DOI":"10.3390\/s19214674","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T11:26:13Z","timestamp":1572261973000},"page":"4674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Estimation of Biological Parameters of Cutaneous Ulcers Caused by Leishmaniasis in an Animal Model Using Diffuse Reflectance Spectroscopy"],"prefix":"10.3390","volume":"19","author":[{"given":"Deivid","family":"Botina","sequence":"first","affiliation":[{"name":"Research group on Advance Materials and Energy MatyEr, Biomaterials and Electromedicine Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Franco","sequence":"additional","affiliation":[{"name":"Research group on Automatic, Electronic and Computational Science, MIRP Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Murillo","sequence":"additional","affiliation":[{"name":"Program for the Study and Control of Tropical Diseases\u2014PECET, School of Medicine, University of Antioquia, Calle 62 No. 52-59, Medell\u00edn 050010, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"July","family":"Galeano","sequence":"additional","affiliation":[{"name":"Research group on Advance Materials and Energy MatyEr, Biomaterials and Electromedicine Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur","family":"Zarzycki","sequence":"additional","affiliation":[{"name":"Research group on Advance Materials and Energy MatyEr, Biomaterials and Electromedicine Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"},{"name":"Research group on Automatic, Electronic and Computational Science, Robotics and Control System Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9795-2459","authenticated-orcid":false,"given":"Maria C.","family":"Torres-Madronero","sequence":"additional","affiliation":[{"name":"Research group on Automatic, Electronic and Computational Science, MIRP Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilo","family":"Berm\u00fadez","sequence":"additional","affiliation":[{"name":"Research group on Advance Materials and Energy MatyEr, Biomaterials and Electromedicine Laboratory, Instituto Tecnol\u00f3gico Metropolitano, Calle 54A No. 30-01, Medell\u00edn 050013, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Monta\u00f1o","sequence":"additional","affiliation":[{"name":"Program for the Study and Control of Tropical Diseases\u2014PECET, School of Medicine, University of Antioquia, Calle 62 No. 52-59, Medell\u00edn 050010, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnson","family":"Garz\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo de \u00d3ptica y Espectroscop\u00eda, Centro de Ciencia B\u00e1sica, Universidad Pontificia Bolivariana, Cq. 1 No. 70-01, Medell\u00edn 050031, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck","family":"Marzani","sequence":"additional","affiliation":[{"name":"Laboratoire ImViA, Universit\u00e9 Bourgogne Franche-Comt\u00e9, B.P. 47870, 21078 Dijon CEDEX, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-4931","authenticated-orcid":false,"given":"Sara M.","family":"Robledo","sequence":"additional","affiliation":[{"name":"Program for the Study and Control of Tropical Diseases\u2014PECET, School of Medicine, University of Antioquia, Calle 62 No. 52-59, Medell\u00edn 050010, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,28]]},"reference":[{"key":"ref_1","unstructured":"(2018, September 30). 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