{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:33:24Z","timestamp":1764084804343,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T00:00:00Z","timestamp":1574640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Nature Science Foundation of China","award":["21565028","21765022","21961042"],"award-info":[{"award-number":["21565028","21765022","21961042"]}]},{"name":"Guangxi Colleges and Universities Program of Innovative Research Team and Outstanding Talent","award":["2014-49"],"award-info":[{"award-number":["2014-49"]}]},{"name":"the Natural Science Foundation of Guangxi","award":["2018GXNSFAA294064"],"award-info":[{"award-number":["2018GXNSFAA294064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A surface-enhanced Raman scattering (SERS) tag is proposed for high-sensitivity detection of gibberellin A3 (GA3). Silver nanoparticles (AgNPs) were synthesized using citrate reduction. 4-Mercaptobenzoic acid (MBA) was used for the Raman-labeled molecules, which were coupled to the surface of the AgNPs using sulfydryls. MBA was coated with silica using the St\u00f6ber method to prevent leakage. GA3 antibodies were attached via the active functional groups N-Hydroxysuccinimide (NHS) and N-Ethyl-N\u2019-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to construct a novel immuno-AgNPs@SiO2 SERS tags. The captured SERS substrates were fabricated through Fe3O4 nanoparticles and gold nanoparticles (AuNPs) using chemical methods. These nanoparticles were characterized using ultraviolet-visible spectroscopy (UV\u2013Vis), dynamic light scattering, Raman spectroscopy, transmission electron microscope (TEM), and X-ray diffraction (XRD). This immuno-AgNPs@SiO2 SERS tags has a strong SERS signal based on characterizations via Raman spectroscopy. Based on antigen-antibody reaction, the immuno-Au@Fe3O4 nanoparticles can capture the GA3 and AgNPs@SiO2 SERS tags. Due to the increasing number of captured nanoprobes, the SERS signal from MBA was greatly enhanced, which favored the sensitive detection of GA3. The linear equation for the SERS signal was y = \u221213635x + 202211 (R2 = 0.9867), and the limit of detection (LOD) was 10\u221210 M. The proposed SERS tags are also applicable for the detection of other food risk factors.<\/jats:p>","DOI":"10.3390\/s19235152","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T05:52:58Z","timestamp":1574661178000},"page":"5152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synthesis of MBA-Encoded Silver\/Silica Core-Shell Nanoparticles as Novel SERS Tags for Biosensing Gibberellin A3 Based on Au@Fe3O4 as Substrate"],"prefix":"10.3390","volume":"19","author":[{"given":"Qingmin","family":"Wei","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fa","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changchun","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science, Guangxi Normal University, Guilin 541004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guobao","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S61","DOI":"10.1105\/tpc.010476","article-title":"Gibberellin signaling: biosynthesis, catabolism, and response pathways","volume":"14","author":"Olszewski","year":"2002","journal-title":"The Plant Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1042\/BJ20120245","article-title":"Gibberellin biosynthesis and its regulation","volume":"444","author":"Hedden","year":"2012","journal-title":"Biochem. 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