{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T02:46:37Z","timestamp":1774406797042,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Information about the characteristics of measuring chips according to their storage conditions is of great importance for clinical diagnosis. In our present work, we have studied the capability of chips to detect nanowire biosensors when they are either freshly prepared or have been stored for either one or two years in a clean room. Potential to detect DNA oligonucleotides (oDNAs)\u2014synthetic analogues of microRNAs (miRNAs) 198 and 429 that are associated with the development of prostate cancer (PCa)\u2014in buffer solution was demonstrated using a nanowire biosensor based on silicon-on-insulator structures (SOI-NW biosensor). To provide biospecific detection, nanowire surfaces were sensitized with oligonucleotide probes (oDNA probes) complimentary to the known sequences of miRNA 183 and 484. In this study it is demonstrated that freshly prepared SOI-NW biosensor chips with n-type conductance and immobilized oDNA probes exhibit responses to the addition of complimentary oDNAs in buffer, leading to decreases in chips\u2019 conductance at a concentration of 3.3 \u00d7 10\u221216 M. The influence of storage time on the characteristics of SOI-NW biosensor chips is also studied herein. It is shown that a two-year storage of the chips leads to significant changes in their characteristics, resulting in \u201cinverse\u201d sensitivity toward negatively charged oDNA probes (i.e., through an increase in chips\u2019 conductance). It is concluded that the surface layer makes the main contribution to conductance of the biosensor chip. Our results indicate that the detection of target nucleic acid molecules can be carried out with high sensitivity using sensor chips after long-term storage, but that changes in their surface properties, which lead to inversed detection signals, must be taken into account. Examples of the applications of such chips for the detection of cancer-associated microRNAs in plasma samples of patients with diagnosed prostate cancer are given. The results obtained herein are useful for the development of highly sensitive nanowire-based diagnostic systems for the revelation of (prostate) cancer-associated microRNAs in human plasma.<\/jats:p>","DOI":"10.3390\/s19235248","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T10:58:21Z","timestamp":1575025101000},"page":"5248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Detection of Marker miRNAs, Associated with Prostate Cancer, in Plasma Using SOI-NW Biosensor in Direct and Inversion Modes"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5041-1914","authenticated-orcid":false,"given":"Yuri","family":"Ivanov","sequence":"first","affiliation":[{"name":"Institute of Biomedical Chemistry (IBMC), Moscow 119121, Russia"},{"name":"Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow 125412, Russia"}]},{"given":"Tatyana","family":"Pleshakova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry (IBMC), Moscow 119121, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9404-1660","authenticated-orcid":false,"given":"Kristina","family":"Malsagova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry (IBMC), Moscow 119121, Russia"}]},{"given":"Leonid","family":"Kurbatov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry (IBMC), Moscow 119121, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7415-1405","authenticated-orcid":false,"given":"Vladimir","family":"Popov","sequence":"additional","affiliation":[{"name":"Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia"}]},{"given":"Alexander","family":"Glukhov","sequence":"additional","affiliation":[{"name":"Joint-Stock Company \u201cNovosibirsk Plant of Semiconductor Devices &amp; DC\u201d, Novosibirsk 630082, Russia"}]},{"given":"Alexander","family":"Smirnov","sequence":"additional","affiliation":[{"name":"Russian Union of Industrialists and Entrepreneurs, Moscow 109240, Russia"}]},{"given":"Dmitry","family":"Enikeev","sequence":"additional","affiliation":[{"name":"Institute for Urology and Reproductive Health, Sechenov University, Moscow 119992, Russia"}]},{"given":"Natalia","family":"Potoldykova","sequence":"additional","affiliation":[{"name":"Institute for Urology and Reproductive Health, Sechenov University, Moscow 119992, Russia"}]},{"given":"Boris","family":"Alekseev","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Moscow 125284, Russia"}]},{"given":"Daniyar","family":"Dolotkazin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Moscow 125284, Russia"}]},{"given":"Andrey","family":"Kaprin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Moscow 125284, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7942-3337","authenticated-orcid":false,"given":"Vadim","family":"Ziborov","sequence":"additional","affiliation":[{"name":"Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow 125412, Russia"}]},{"given":"Oleg","family":"Petrov","sequence":"additional","affiliation":[{"name":"Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow 125412, Russia"}]},{"given":"Alexander","family":"Archakov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Chemistry (IBMC), Moscow 119121, Russia"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.18097\/PBMC20146002141","article-title":"Modern approach to breast cancer diagnostics","volume":"60","author":"Tamkovich","year":"2014","journal-title":"Biomed Khim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/00006676-200404000-00011","article-title":"Diagnostic imaging for pancreatic cancer: Computed tomography, magnetic resonance imaging, and positron emission tomography","volume":"28","author":"Saisho","year":"2004","journal-title":"Pancreas"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.bios.2012.11.028","article-title":"Label-free electrochemical impedance spectroscopy biosensor for direct detection of cancer cells based on the interaction between carbohydrate and lectin","volume":"43","author":"Hu","year":"2013","journal-title":"Biosens. 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