{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:52:41Z","timestamp":1760241161876,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"New Energy and Industrial Technology Development Organization","award":["16100863-0"],"award-info":[{"award-number":["16100863-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The saltiness enhancement effect is the effect whereby saltiness is enhanced by adding specific substances to salt (sodium chloride). Since this effect can be used in the development of salt-reduced foods, a method to objectively evaluate the saltiness with this effect is required. A taste sensor with lipid\/polymer membranes has been used to quantify the taste of food and beverages in recent years. The sensor electrodes of this taste sensor have the feature of selectively responding to each of the five basic tastes, which is realized by the lipid\/polymer membranes. In this study, we developed a new saltiness sensor based on the lipid\/polymer membrane with the aim of quantifying the saltiness enhancement effect. In addition to the conventional components of a lipid, plasticizer, and polymer supporting reagent, the membrane we developed comprises ionophores, which selectively capture sodium ions. As a result, the response of the sensor increased logarithmically with the activity of NaCl in measured samples, similarly to the taste response of humans. In addition, all of the sensor responses increased upon adding saltiness-enhancing substances, such as citric acid, tartaric acid and branched-chain amino acids (BCAAs), to NaCl samples. These findings suggest that it is possible to quantify the saltiness enhancement effect using a taste sensor with lipid\/polymer membranes.<\/jats:p>","DOI":"10.3390\/s19235251","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T10:58:21Z","timestamp":1575025101000},"page":"5251","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of a Sensor with a Lipid\/Polymer Membrane Comprising Na+ Ionophores to Evaluate the Saltiness Enhancement Effect"],"prefix":"10.3390","volume":"19","author":[{"given":"Futa","family":"Nakatani","sequence":"first","affiliation":[{"name":"Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomofumi","family":"Ienaga","sequence":"additional","affiliation":[{"name":"Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Wu","sequence":"additional","affiliation":[{"name":"Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Tahara","sequence":"additional","affiliation":[{"name":"Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidekazu","family":"Ikezaki","sequence":"additional","affiliation":[{"name":"Intelligent Sensor Technology, Inc., 5-1-1 Onna, Atsugi-shi, Kanagawa 243-0032, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Sano","sequence":"additional","affiliation":[{"name":"Fuji Foods Corp., 5-14 Hanedaasahi-cho, Ota-ku, Tokyo 144-0042, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Muto","sequence":"additional","affiliation":[{"name":"Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Kaneda","sequence":"additional","affiliation":[{"name":"Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Toko","sequence":"additional","affiliation":[{"name":"Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"},{"name":"Institute for Advanced Study, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1001\/archinte.1954.00250040017003","article-title":"Evidence for relationship sodium (chloride) intake and human essential hypertension","volume":"94","author":"Dahl","year":"1954","journal-title":"Arch. Intern. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1541-4337.2009.00096.x","article-title":"Sodium reduction and its effect on food safety, food quality, and human health","volume":"9","author":"Doyle","year":"2010","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_3","first-page":"1625","article-title":"An enhancing effect on the saltiness of sodium chloride of added amino acids and their esters","volume":"53","author":"Tamura","year":"1989","journal-title":"Agric. Biol. Chem."},{"key":"ref_4","unstructured":"Japan Tobacco Inc. (2008). Seasoning Composition, Salty Taste-Like Taste Enhancer and Method for Enhancing Salty Taste-Like Taste of Food and Drink. (2138053A4), E.P. Patent."},{"key":"ref_5","first-page":"441","article-title":"Utilization of L-leucine can reduce the salt content in food","volume":"14","author":"Harada","year":"2007","journal-title":"Jpn. J. Tast. Smell Res."},{"key":"ref_6","unstructured":"Hiroshima University (2008). Salty Taste Enhancer, Food and Drink and Method for Producing Food and Drink. (20100075017A1), U.S. Patent."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"274","DOI":"10.3136\/nskkk.62.274","article-title":"Screening of salt taste enhancing dipeptides based on a new strategy using L-amino acid ligase","volume":"62","author":"Kino","year":"2015","journal-title":"Nippon Shokuhin Kagaku Kogaku Kaishi"},{"key":"ref_8","first-page":"219","article-title":"Novel development of ume salt enhanced saltiness","volume":"67","author":"Ishikawa","year":"2013","journal-title":"Bull. Soc. Sea Water Sci. Jpn."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"12578","DOI":"10.1021\/jf2041593","article-title":"Discovery of salt taste enhancing arginyl dipeptides in protein digests and fermented fish sauces by means of a sensomics approach","volume":"59","author":"Schindler","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1093\/chemse\/bju051","article-title":"Sodium aspartate as a specific enhancer of salty taste perception-sodium aspartate is a possible candidate to decrease excessive intake of dietary salt","volume":"39","author":"Nakagawa","year":"2014","journal-title":"Chem. Senses"},{"key":"ref_11","first-page":"151","article-title":"Application of the linking response of taste and odor to salt-reduced food","volume":"22","author":"Shimoda","year":"2015","journal-title":"Jpn. J. Tast. Smell Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.foodqual.2008.10.004","article-title":"Odour-taste interactions: A way to enhance saltiness in low-salt content solutions","volume":"20","author":"Lawrence","year":"2009","journal-title":"Food Qual. Prefer."},{"key":"ref_13","first-page":"3","article-title":"Does shoyu odor strengthen salty flavor?","volume":"14","author":"Shimoda","year":"2007","journal-title":"Jpn. J. Tast. Smell Res."},{"key":"ref_14","first-page":"5","article-title":"Enhancement effect of salt taste based on flavor technology","volume":"22","author":"Miyazawa","year":"2015","journal-title":"Jpn. J. Tast. Smell Res."},{"key":"ref_15","first-page":"22","article-title":"The enhancing effect of saltiness in foods and the estimation","volume":"112","author":"Kawai","year":"2017","journal-title":"Nippon Jozokyokai Shi"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.drudis.2007.01.010","article-title":"The latest trends in the taste assessment of pharmaceuticals","volume":"12","author":"Anand","year":"2007","journal-title":"Drug Discov. Today"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.1039\/c0an00292e","article-title":"Recent advances in electronic tongues","volume":"135","author":"Riul","year":"2010","journal-title":"Analyst"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Toko, K. (2000). Biomimetic Sensor Technology, Cambridge University Press.","DOI":"10.1017\/CBO9780511541179"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.3390\/s100403411","article-title":"Advanced taste sensors based on artificial lipids with global selectivity to basic taste qualities and high correlation to sensory scores","volume":"10","author":"Kobayashi","year":"2010","journal-title":"Sensors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1109\/JSEN.2013.2263125","article-title":"Electronic tongues-a review","volume":"13","author":"Tahara","year":"2013","journal-title":"IEEE Sens. J."},{"key":"ref_21","first-page":"2347","article-title":"Identification of Xinyang Maojian Tea Taste Using Electronic Tongue","volume":"31","author":"Zhang","year":"2019","journal-title":"Sens. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1248\/cpb.c17-00619","article-title":"The utility of the artificial taste sensor in evaluating the bitterness of drugs: Correlation with responses of human TASTE2 receptors (hTAS2Rs)","volume":"66","author":"Haraguchi","year":"2018","journal-title":"Chem. Pharm. Bull."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00604-007-0929-2","article-title":"Voltammetric electronic tongues\u2014Basic principles and applications","volume":"163","author":"Winquist","year":"2008","journal-title":"Microchim. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.procs.2015.10.062","article-title":"Development of lipid membrane based taste sensors for electronic tongue","volume":"70","author":"Sharma","year":"2015","journal-title":"Procedia Comput. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1039\/b705107g","article-title":"Sensor arrays for liquid sensing\u2014electronic tongue systems","volume":"132","author":"Ciosek","year":"2007","journal-title":"Analyst"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1351\/pac200577111965","article-title":"Nonspecific sensor arrays (\u201celectronic tongue\u201d) for chemical analysis of liquids","volume":"77","author":"Vlasov","year":"2005","journal-title":"Pure Appl. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1021\/ac086073z","article-title":"Smart taste sensors","volume":"80","author":"Citterio","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.microc.2013.04.018","article-title":"Development and application of an electronic tongue for detection and monitoring of nitrate, nitrite and ammonium levels in waters","volume":"110","author":"Pividori","year":"2013","journal-title":"Microchem. J."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Podrazka, M., B\u00e1czy\u0144ska, E., Kundys, M., Jele\u0144, P.S., and Nery, E.W. (2018). Electronic tongue\u2014A tool for all tastes?. Biosensors, 8.","DOI":"10.3390\/bios8010003"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.snb.2016.05.009","article-title":"Quantitative prediction of bitterness masking effect of high-potency sweeteners using taste sensor","volume":"235","author":"Wu","year":"2016","journal-title":"Sens. Actuators B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0925-4005(99)00508-0","article-title":"Taste sensor","volume":"64","author":"Toko","year":"2000","journal-title":"Sens. Actuators B"},{"key":"ref_32","first-page":"313","article-title":"Development of sensor with high selectivity for saltiness and its application in taste evaluation of table salt","volume":"22","author":"Chen","year":"2010","journal-title":"Sens. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.1021\/cr940394a","article-title":"Carrier-based ion-selective electrodes and bulk optodes. 1. General characteristics","volume":"97","author":"Bakker","year":"1997","journal-title":"Chem. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2116\/analsci.12.67","article-title":"Effects of \u03b1-substituents on ion selectivity of bis(12-crown-4-methyl) malonates as neutral carriers for sodium ion-selective electrodes","volume":"12","author":"Kimura","year":"1996","journal-title":"Anal. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"136","DOI":"10.4992\/psycholres1954.8.136","article-title":"A general equi-distance scale of the four qualities of taste","volume":"8","author":"Indow","year":"1966","journal-title":"Jpn. Psychol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"769","DOI":"10.3136\/nskkk1962.36.9_769","article-title":"An enhanced effect of saltiness of sodium chloride by peptides and their analogs","volume":"36","author":"Okai","year":"1989","journal-title":"Nippon Shokuhin Kogyo Gakkaishi"},{"key":"ref_37","first-page":"91","article-title":"Biomimetic membrane for taste sensing","volume":"Volume 6","author":"Ariga","year":"2009","journal-title":"Bottom-Up Nanofabrication"},{"key":"ref_38","unstructured":"Kolpin, K.M. (2008). Human Bitterness Detection Thresholds of Hop Acids in Beer and Honey. [Master\u2019s Thesis, Oregon State University]."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"529S","DOI":"10.1093\/jn\/136.2.529S","article-title":"Nutraceutical effects of branched-chain amino acids on skeletal muscle","volume":"136","author":"Shimomura","year":"2006","journal-title":"J. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1016\/S0016-5085(03)00323-8","article-title":"Nutritional supplementation with branched-chain amino acids in advanced cirrhosis: A double-blind, randomized trial","volume":"124","author":"Marchesini","year":"2003","journal-title":"Gastroenterology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0301-4622(76)80013-0","article-title":"Electrostatic interactions at charged lipid membranes: I. Effects of pH and univalent cations on membrane structure","volume":"4","author":"Teubner","year":"1976","journal-title":"Biophys. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/23\/5251\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:38:33Z","timestamp":1760189913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/23\/5251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,29]]},"references-count":41,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["s19235251"],"URL":"https:\/\/doi.org\/10.3390\/s19235251","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2019,11,29]]}}}