{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T05:54:26Z","timestamp":1772690066174,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007049","name":"Korea Institute of Ocean Science and Technology","doi-asserted-by":"publisher","award":["PE99721"],"award-info":[{"award-number":["PE99721"]}],"id":[{"id":"10.13039\/501100007049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.<\/jats:p>","DOI":"10.3390\/s19235298","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T10:50:45Z","timestamp":1575283845000},"page":"5298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives"],"prefix":"10.3390","volume":"19","author":[{"given":"Suji","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea"}]},{"given":"Dan-Bi","family":"Sung","sequence":"additional","affiliation":[{"name":"Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology (KIOST), Busan 49111, Korea"}]},{"given":"Seungyoon","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9279-2620","authenticated-orcid":false,"given":"Saravanan","family":"Parameswaran","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea"}]},{"given":"Jun-Ho","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3892-3439","authenticated-orcid":false,"given":"Jong Seok","family":"Lee","sequence":"additional","affiliation":[{"name":"Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology (KIOST), Busan 49111, Korea"},{"name":"Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Korea"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9588-6980","authenticated-orcid":false,"given":"Min Su","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/S0021-9258(17)37930-9","article-title":"Human Serum Albumin. 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