{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:20:27Z","timestamp":1761294027097,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Dielectrophoresis is an electric force experienced by particles subjected to non-uniform electric fields. Recently, several technologies have been developed focused on the use of dielectrophoretic force (DEP) to manipulate and detect cells. On the other hand, there is no such great development in the field of DEP-based cell discrimination methods. Despite the demand for methods to differentiate biological cell states, most DEP developed methods have been focused on differentiation through geometric parameters. The novelty of the present work relies upon the point that a DEP force cell measurement is used as a discrimination method, capable of detecting heat killed yeast cells from the alive ones. Thermal treatment is used as an example of different biological state of cells. It comes from the fact that biological properties have their reflection in the electric properties of the particle, in this case a yeast cell. To demonstrate such capability of the method, 279 heat-killed cells were measured and compared with alive cells data from the literature. For each cell, six speeds were taken at different points in its trajectory inside a variable non-uniform electric field. The electric parameters in cell wall conductivity, cell membrane conductivity, cell membrane permittivity of the yeast cell from bibliography explains the DEP experimental force measured. Finally, alive and heat-treated cells were distinguished based on that measure. Our results can be explained through the well-known damage of cell structure characteristics of heat-killed cells.<\/jats:p>","DOI":"10.3390\/s19235304","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T04:58:39Z","timestamp":1575349119000},"page":"5304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7062-245X","authenticated-orcid":false,"given":"Garc\u00eda-Diego","family":"Fernando-Juan","sequence":"first","affiliation":[{"name":"ACUMA Research Centre Universitat Polit\u00e8cnica de Val\u00e8ncia, Av. de los Naranjos s\/n, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2783-9909","authenticated-orcid":false,"given":"Mario","family":"Rubio-Chavarr\u00eda","sequence":"additional","affiliation":[{"name":"Escuela T\u00e9cnica Superior de Ingenieros Industriales Universitat Polit\u00e8cnica de Val\u00e8ncia, Av.de los Naranjos s\/n, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"ACUMA Research Centre Universitat Polit\u00e8cnica de Val\u00e8ncia, Av. de los Naranjos s\/n, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco J.","family":"Espin\u00f3s","sequence":"additional","affiliation":[{"name":"ACUMA Research Centre Universitat Polit\u00e8cnica de Val\u00e8ncia, Av. de los Naranjos s\/n, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1149\/2.0071705jes","article-title":"Review\u2014Where Is Dielectrophoresis (DEP) Going?","volume":"164","author":"Pethig","year":"2017","journal-title":"J. Electrochem. Soc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.1016\/j.mee.2010.01.013","article-title":"A modular micro-fluidic platform for cells handling by dielectrophoresis","volume":"87","author":"Burgarella","year":"2010","journal-title":"Microelectron. Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1002\/elps.201400481","article-title":"A portable and integrated instrument for cell manipulation by dielectrophoresis: Microfluidics and Miniaturization","volume":"36","author":"Burgarella","year":"2015","journal-title":"Electrophoresis"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s00216-013-7437-5","article-title":"Differentiation of Escherichia coli serotypes using DC gradient insulator dielectrophoresis","volume":"406","author":"Jones","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1039\/C4LC01253D","article-title":"Continuous-flow sorting of stem cells and differentiation products based on dielectrophoresis","volume":"15","author":"Song","year":"2015","journal-title":"Lab. Chip"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"125501","DOI":"10.1088\/0957-4484\/26\/12\/125501","article-title":"Dielectrophoretic stretching of DNA tethered to a fiber tip","volume":"26","author":"Hyun","year":"2015","journal-title":"Nanotechnology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"054020","DOI":"10.1088\/0960-1317\/21\/5\/054020","article-title":"Single-DNA-molecule trapping with silicon nanotweezers using pulsed dielectrophoresis","volume":"21","author":"Kumemura","year":"2011","journal-title":"J. Micromech. Microeng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2217\/ijh-2015-0009","article-title":"Dielectrophoretic recovery of DNA from plasma for the identification of chronic lymphocytic leukemia point mutations","volume":"5","author":"Manouchehri","year":"2016","journal-title":"Int. J. Hematol. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1002\/elps.201200444","article-title":"Dielectrophoretic isolation and detection of cfc-DNA nanoparticulate biomarkers and virus from blood: Nucleic Acids","volume":"34","author":"Sonnenberg","year":"2013","journal-title":"Electrophoresis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1039\/C5AN02430G","article-title":"Concentration of Sindbis virus with optimized gradient insulator-based dielectrophoresis","volume":"141","author":"Ding","year":"2016","journal-title":"Analyst"},{"doi-asserted-by":"crossref","unstructured":"Morimoto, A., Mogami, T., Watanabe, M., Lijima, K., Akiyama, Y., Katayama, K., Futami, T., Yamamoto, N., Sawada, T., and Koizumi, F. (2015). High-Density Dielectrophoretic Microwell Array for Detection, Capture, and Single-Cell Analysis of Rare Tumor Cells in Peripheral Blood. PLoS ONE, 10.","key":"ref_11","DOI":"10.1371\/journal.pone.0130418"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"011803","DOI":"10.1063\/1.4774312","article-title":"Separation of tumor cells with dielectrophoresis-based microfluidic chip","volume":"7","author":"Alshareef","year":"2013","journal-title":"Biomicrofluidics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.talanta.2016.11.008","article-title":"Lab-on-chip for liquid biopsy (LoC-LB) based on dielectrophoresis","volume":"64","author":"Mathew","year":"2017","journal-title":"Talanta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"233501","DOI":"10.1063\/1.4984897","article-title":"Lab-on-chip microfluidic impedance measurement for laminar flow ratio sensing and differential conductivity difference detection","volume":"110","author":"Kong","year":"2017","journal-title":"Appl. Phys. Lett."},{"doi-asserted-by":"crossref","unstructured":"Nakano, M., Obara, R., Ding, Z., and Suehiro, J. (2013, January 3\u20135). Detection of norovirus and rotavirus by dielectrophoretic impedance measurement. Proceedings of the Seventh International Conference on Sensing Technology (ICST), Wellington, New Zealand.","key":"ref_15","DOI":"10.1109\/ICSensT.2013.6727678"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"017001","DOI":"10.7567\/JJAP.55.017001","article-title":"Dielectrophoresis and dielectrophoretic impedance detection of adenovirus and rotavirus","volume":"55","author":"Nakano","year":"2016","journal-title":"Jpn. J. Appl. Phys."},{"doi-asserted-by":"crossref","unstructured":"Heller, M.J., Krishnan, R., and Sonnenberg, A. (2010, January 1\u20135). Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. Proceedings of the SPIE NanoScience + Engineering, San Diego, CA, USA.","key":"ref_17","DOI":"10.1117\/12.861579"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1002\/elps.201400016","article-title":"Dielectrophoretic isolation and detection of cancer-related circulating cell-free DNA biomarkers from blood and plasma: Nucleic acids","volume":"35","author":"Sonnenberg","year":"2014","journal-title":"Electrophoresis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1002\/elps.201100081","article-title":"Selective concentration of human cancer cells using contactless dielectrophoresis","volume":"32","author":"Henslee","year":"2011","journal-title":"Electrophoresis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1039\/c1lc20307j","article-title":"Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity","volume":"11","author":"Park","year":"2011","journal-title":"Lab. Chip"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1021\/cm500062b","article-title":"Rapid and Sensitive in Situ SERS Detection Using Dielectrophoresis","volume":"26","author":"Cherukulappurath","year":"2014","journal-title":"Chem. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"R73","DOI":"10.1088\/0967-3334\/26\/3\/R02","article-title":"Microfluidics for flow cytometric analysis of cells and particles","volume":"26","author":"Huh","year":"2005","journal-title":"Physiol. Meas."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1002\/elps.201500282","article-title":"Discriminating dengue-infected hepatic cells (WRL-68) using dielectrophoresis: Microfluidics and Miniaturization","volume":"37","author":"Yafouz","year":"2016","journal-title":"Electrophoresis"},{"doi-asserted-by":"crossref","unstructured":"Abd Rahman, N., Ibrahim, F., and Yafouz, B. (2017). Dielectrophoresis for Biomedical Sciences Applications: A Review. Sensors, 17.","key":"ref_24","DOI":"10.3390\/s17030449"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1039\/C3IB40255J","article-title":"Human oral cancer cells with increasing tumorigenic abilities exhibit higher effective membrane capacitance","volume":"6","author":"Liang","year":"2014","journal-title":"Integr. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/term.1629","article-title":"Characterization of human skeletal stem and bone cell populations using dielectrophoresis: Dielectrophoretic properties of skeletal populations","volume":"9","author":"Ismail","year":"2015","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1002\/smll.201202195","article-title":"Acute On-Chip HIV Detection Through Label-Free Electrical Sensing of Viral Nano-Lysate","volume":"9","author":"Shafiee","year":"2013","journal-title":"Small"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1109\/JERM.2019.2895539","article-title":"UHF-Dielectrophoresis Crossover Frequency as a New Marker for Discrimination of Glioblastoma Undifferentiated Cells","volume":"3","author":"Manczak","year":"2019","journal-title":"IEEE J. Electromagn. RF Microw. Med. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1634\/stemcells.2007-0810","article-title":"Unique Dielectric Properties Distinguish Stem Cells and Their Differentiated Progeny","volume":"26","author":"Flanagan","year":"2008","journal-title":"Stem Cells"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12","DOI":"10.14429\/dlsj.4.12572","article-title":"Advances in Rapid Detection and Antimicrobial Susceptibility Tests: A Review","volume":"4","author":"Mishra","year":"2018","journal-title":"Def. Life Sci. J."},{"unstructured":"Cruz, J.M., and Garcia-Diego, F.-J. (1997, January 5\u20139). Dielectrophoretic force measurements in yeast cells by the Stokes method. Proceedings of the IAS\u201997. Conference Record of the 1997 IEEE Industry Applications Conference Thirty-Second IAS Annual Meeting, New Orleans, LA, USA.","key":"ref_31"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1088\/0022-3727\/31\/14\/020","article-title":"Dielectrophoretic motion of oblate spheroidal particles. Measurements of motion of red blood cells using the Stokes method","volume":"31","author":"Cruz","year":"1998","journal-title":"J. Phys. Appl. Phys."},{"doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Diego, F.-J., Rubio-Chavarr\u00eda, M., Beltr\u00e1n, P., and Espin\u00f3s, F.J. (2019). Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement. Sensors, 19.","key":"ref_33","DOI":"10.3390\/s19173813"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s10544-018-0341-1","article-title":"Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies","volume":"20","author":"Kayani","year":"2018","journal-title":"Biomed. Microdevices"},{"doi-asserted-by":"crossref","unstructured":"Huang, K., Chu, H.K., Lu, B., Lai, J., and Cheng, L. (2019, January 20\u201324). Automated Cell Patterning System with a Microchip using Dielectrophoresis. Proceedings of the International Conference on Robotics and Automation (ICRA), Montreal, QC, Canada.","key":"ref_35","DOI":"10.1109\/ICRA.2019.8794177"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1002\/tee.22979","article-title":"2D Dielectrophoresis using an active matrix array made by thin-film-transistor technology","volume":"14","author":"Ihida","year":"2019","journal-title":"IEEJ Trans. Electr. Electron. Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11461","DOI":"10.1021\/acs.analchem.8b02628","article-title":"High-Throughput Separation, Trapping, and Manipulation of Single Cells and Particy Combined Dielectrophoresis at a Bipolar Electrode Array","volume":"90","author":"Wu","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1002\/elps.201700395","article-title":"Enhanced cell trapping throughput using DC-biased AC electric field in a dielectrophoresis-based fluidic device with densely packed silica beads","volume":"39","author":"Lewpiriyawong","year":"2018","journal-title":"Electrophoresis"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1021\/acs.analchem.9b01104","article-title":"Continuous Cell Characterization and Separation by Microfluidic Alternating Current Dielectrophoresis","volume":"91","author":"Zhao","year":"2019","journal-title":"Anal. Chem."},{"doi-asserted-by":"crossref","unstructured":"Luu, Y., and Macreadie, I. (2018). Development of Convenient System for Detecting Yeast Cell Stress, Including That of Amyloid Beta. Int. J. Mol. Sci., 19.","key":"ref_40","DOI":"10.3390\/ijms19072136"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1016\/j.snb.2017.08.179","article-title":"Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy","volume":"255","author":"Li","year":"2018","journal-title":"Sensors Actuators B Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13568-014-0040-0","article-title":"Effects of biosurfactants on the viability and proliferation of human breast cancer cells","volume":"4","author":"Duarte","year":"2014","journal-title":"AMB Express"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/MMBR.05018-11","article-title":"Biology of the Heat Shock Response and Protein Chaperones: Budding Yeast (Saccharomyces cerevisiae) as a Model System","volume":"76","author":"Verghese","year":"2012","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1466-8564(01)00020-0","article-title":"Effect of ultrasound on the survival of Saccharomyces cere\u00a8visiae: influence of temperature, pH and amplitude","volume":"2","author":"Guerrero","year":"2001","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.addr.2013.11.008","article-title":"Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms","volume":"72","author":"Lentacker","year":"2014","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0005-2736(92)90150-K","article-title":"Dielectric properties of yeast cells as determined by electrorotation","volume":"1104","author":"Lamprecht","year":"1992","journal-title":"Biochim. Biophys. Acta"},{"unstructured":"Pohl, H.A. (1978). Dielectrophoresis: The Behavior of Neutral Matter in Nonuniform Electric Fields, Cambridge University Press.","key":"ref_47"},{"doi-asserted-by":"crossref","unstructured":"Jones, T.B. (1995). Electromechanics of Particles, Cambridge University Press.","key":"ref_48","DOI":"10.1017\/CBO9780511574498"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/j.2050-0416.1970.tb03325.x","article-title":"For the Analysis Committee. Institute of brewing analysis committee: Measurement of yeast viability","volume":"76","author":"Pierce","year":"1970","journal-title":"J. Inst. Brew."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0003-2697(91)90013-J","article-title":"Direct detection of yeast mutants with reduced viability on plates by erythrosine B staining","volume":"193","author":"Bonneu","year":"1991","journal-title":"Anal. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1049\/ip-smt:19941073","article-title":"Optical technique for measuring the positive and negative dielectrophoretic behaviour of cells and colloidal suspensions","volume":"141","author":"Talary","year":"1994","journal-title":"IEE Proc. Sci. Meas. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.1016\/S0006-3495(96)79420-1","article-title":"Dielectric Behavior of Wild-Type Yeast and Vacuole-Deficient Mutant Over a Frequency Range of 10 kHz to 10 GHz","volume":"71","author":"Asami","year":"1996","journal-title":"Biophys. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0006-3495(03)74515-9","article-title":"Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis","volume":"85","author":"Pavlin","year":"2003","journal-title":"Biophys. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0006-3495(91)82212-3","article-title":"Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential","volume":"59","author":"Hibino","year":"1991","journal-title":"Biophys. J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1088\/0031-9155\/37\/7\/003","article-title":"Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies","volume":"37","author":"Huang","year":"1992","journal-title":"Phys. Med. Biol."},{"key":"ref_56","first-page":"1817","article-title":"Dielectric properties of single cells subjected to heat shock using DEP cytometry","volume":"66","author":"Afshar","year":"2018","journal-title":"TMTT"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1088\/0031-9155\/33\/5\/004","article-title":"Influence of hyperthermia, pH and culturing conditions on the electrical parameters of Chinese hamster V79 cells","volume":"33","author":"Bonincontro","year":"1988","journal-title":"Phys. Med. Biol."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/23\/5304\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:39:22Z","timestamp":1760189962000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/23\/5304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,2]]},"references-count":57,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["s19235304"],"URL":"https:\/\/doi.org\/10.3390\/s19235304","relation":{},"ISSN":["1424-8220"],"issn-type":[{"type":"electronic","value":"1424-8220"}],"subject":[],"published":{"date-parts":[[2019,12,2]]}}}