{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:47:14Z","timestamp":1773384434248,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Environment Industry &amp; Technology Institute","award":["2016001970001"],"award-info":[{"award-number":["2016001970001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN\u2212. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 \u00b1 0.05 \u03bcM) for zinc ion was far below the standard (76 \u03bcM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN\u2212 through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN\u2212 were demonstrated with various analytical methods like Job plots, 1H NMR titrations, and ESI-Mass analyses.<\/jats:p>","DOI":"10.3390\/s19245458","type":"journal-article","created":{"date-parts":[[2019,12,12]],"date-time":"2019-12-12T03:20:16Z","timestamp":1576120816000},"page":"5458","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN\u2212: Imaging Applications in Live Cells and Zebrafish"],"prefix":"10.3390","volume":"19","author":[{"given":"Min Seon","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul 01187, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongju","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul 01187, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Byeong","family":"Chae","sequence":"additional","affiliation":[{"name":"Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul 01187, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haeri","family":"So","sequence":"additional","affiliation":[{"name":"Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul 01187, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyojin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Environmental Engineering, Seoul National University of Science and Technology, Seoul 01187, Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki-Tae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Environmental Engineering, Seoul National University of Science and Technology, Seoul 01187, Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingeun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 34140, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3377-4996","authenticated-orcid":false,"given":"Mi Hee","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 34140, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheal","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul 01187, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.1021\/cr100383r","article-title":"Recent trends in macro-, micro-, and nanomaterial-based tools and strategies for heavy-metal detection","volume":"111","author":"Aragay","year":"2011","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4517","DOI":"10.1039\/C5CS00005J","article-title":"Photoluminescence imaging of Zn2+ in living systems","volume":"44","author":"Chen","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1039\/b916287a","article-title":"Fluorescent chemosensors for Zn2+","volume":"39","author":"Xu","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.bios.2015.09.059","article-title":"Triphenylamine-based Schiff bases as the High sensitive Al3+ or Zn2+ fluorescence turn-on probe: Mechanism and application in vitro and in vivo","volume":"77","author":"Li","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.dyepig.2012.08.009","article-title":"A highly selective \u201cturn-on\u201d fluorescent chemosensor based on hydroxy pyrene-hydrazone derivative for Zn2+","volume":"96","author":"Choi","year":"2013","journal-title":"Dyes Pigm."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Hagimori, M., Taniura, M., Mizuyama, N., Karimine, Y., Kawakami, S., Saji, H., and Mukai, T. (2019). Synthesis of a novel pyrazine-pyridone biheteroaryl-based fluorescence sensor and detection of endogenous labile zinc ions in lung cancer cells. Sensors, 19.","DOI":"10.3390\/s19092049"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1038\/nprot.2014.086","article-title":"Synthesis of a highly Zn2+-selective cyanine-based probe and its use for tracing endogenous zinc ions in cells and organisms","volume":"9","author":"Guo","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.snb.2017.05.015","article-title":"A single chemosensor with combined ionophore\/fluorophore moieties acting as a fluorescent \u201cOff-On\u201d Zn2+ sensor and a colorimetric sensor for Cu2+: Experimental, logic gate behavior and TD-DFT calculations","volume":"250","author":"Sohrabi","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.snb.2017.05.178","article-title":"Macrocycle aza-crown chromogenic reagent to Al3+ and fluorescence sensor for Zn2+ and Al3+ along with live cell application and logic operation","volume":"252","author":"Bhanja","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s10895-016-1964-3","article-title":"A Colorimetric and Fluorescent Chemosensor for the Selective Detection of Cu2+ and Zn2+ Ions","volume":"27","author":"Kim","year":"2016","journal-title":"J. Fluoresc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.saa.2018.12.053","article-title":"Fluorescein derived Schiff base as fluorimetric zinc(II) sensor via \u2018turn on\u2019 response and its application in live cell imaging","volume":"212","author":"Das","year":"2019","journal-title":"Spectrochim. Acta A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.snb.2017.05.186","article-title":"Ratiometric & reversible fluorescent sensing of NaCN by an effective probe based on phenanthro [9,10-d]imidazole platform","volume":"253","author":"Yang","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.snb.2016.02.014","article-title":"A modest colorimetric chemosensor for investigation of CN\u2212 in semi-aqueous environment with high selectivity and sensitivity","volume":"229","author":"Maurya","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1039\/C5DT03209A","article-title":"Colorimetric detection of fluoride ions by anthraimidazoledione based sensors in the presence of Cu(II) ions","volume":"45","author":"Sarkar","year":"2016","journal-title":"Dalton Trans."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.snb.2015.01.093","article-title":"A multifunctional colorimetric chemosensor for cyanide and copper(II) ions","volume":"211","author":"Jo","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"9120","DOI":"10.1039\/C5DT00772K","article-title":"A colorimetric sensor for the sequential detection of Cu2+ and CN\u2212 in fully aqueous media: Practical performance of Cu2+","volume":"44","author":"You","year":"2015","journal-title":"Dalton Trans."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2549","DOI":"10.1039\/C5DT04039F","article-title":"Triarylborane substituted naphthalimide as a fluoride and cyanide ion sensor","volume":"45","author":"Maragani","year":"2016","journal-title":"Dalton Trans."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.snb.2015.11.122","article-title":"A fluorescence turn-on probe for a catalytic amount of cyanides through the cyanide-mediated cinnamate-to-coumarin transformation","volume":"226","author":"Kim","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/S0039-9140(98)00314-2","article-title":"Continuous monitoring for cyanide in waste water with a galvanic hydrogen cyanide sensor using a purge system","volume":"48","author":"Hachiya","year":"1999","journal-title":"Talanta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.snb.2016.06.115","article-title":"Naphthalene based lab-on-a-molecule for the highly selective and sensitive detection of CN\u2212 and Ag+ in aqueous solution","volume":"237","author":"Zhang","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Elsafy, A.G., Al-Easa, H.S., and Hijji, Y.M. (2018). Substituted 2-aminobenzothiazoles salicylidenes synthesis and characterization as cyanide sensors in aqueous medium. Sensors, 18.","DOI":"10.3390\/s18072219"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1039\/C4DT03247K","article-title":"A highly selective fluorescent \u2018turn-on\u2019 chemosensor for Zn2+ based on a benzothiazole conjugate: Their applicability in live cell imaging and use of the resultant complex as a secondary sensor of CN\u2212","volume":"44","author":"Khairnar","year":"2015","journal-title":"Dalton Trans."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"(1990). \u201cFirst pass phenomenon\u201d of inhaled gas in the fire victims. Forensic Sci. Int., 46, 203\u2013208.","DOI":"10.1016\/0379-0738(90)90306-J"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1016\/j.dyepig.2017.11.051","article-title":"Highly sensitive and selective turn-on fluorescent sensor for dual recognition of Cu2+ and CN\u2212 based on a methylquinoline derivative","volume":"149","author":"Shi","year":"2018","journal-title":"Dyes Pigm."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"16586","DOI":"10.1039\/C5RA27553A","article-title":"Colorimetric chemosensor for multiple targets, Cu2+, CN\u2212 and S2\u2212","volume":"6","author":"Ryu","year":"2016","journal-title":"RSC Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.saa.2016.02.008","article-title":"Several hemicyanine dyes as fluorescence chemosensors for cyanide anions","volume":"169","author":"Liang","year":"2016","journal-title":"Spectrochim. Acta A"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.bios.2017.02.006","article-title":"Highly selective optical and reversible dual-path chemosensor for cyanide detection and its application in live cells imaging","volume":"92","author":"Keshav","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.dyepig.2016.11.002","article-title":"Two-dimensional carbazole-based derivatives as versatile chemosensors for colorimetric detection of cyanide and two-photon fluorescence imaging of viscosity in vitro","volume":"137","author":"Zhang","year":"2017","journal-title":"Dyes Pigm."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1016\/j.tet.2014.02.055","article-title":"A colorimetric and fluorescent sensor for sequential detection of copper ion and cyanide","volume":"70","author":"Park","year":"2014","journal-title":"Tetrahedron"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.inoche.2019.05.026","article-title":"1, 8-Naphthalimide-based fluorescent sensor with highly selective and sensitive detection of Zn2+ in aqueous solution and living cells","volume":"106","author":"Liu","year":"2019","journal-title":"Inorg. Chem. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"19077","DOI":"10.1039\/c4ra01598c","article-title":"Coumarin-hemicyanine conjugates as novel reaction-based sensors for cyanide detection: Convenient synthesis and ICT mechanism","volume":"4","author":"Peng","year":"2014","journal-title":"RSC Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.snb.2019.04.066","article-title":"Orange emitting fluorescence probe for the selective detection of cyanide ion in solution and solid states","volume":"291","author":"Suganya","year":"2019","journal-title":"Sens. Actuators B"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1039\/C7NJ04216G","article-title":"Studies on a multifunctional chromo-fluorogenic sensor for dual channel recognition of Zn2+ and CN\u2212 ions in aqueous media: Mimicking multiple molecular logic gates and memory devices","volume":"42","author":"Rezaeian","year":"2018","journal-title":"New J. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1016\/j.tet.2019.03.031","article-title":"A highly selective diarylethene chemosensor for dual channel recognition of CN\u2212 and Zn2+ and its application","volume":"75","author":"Gao","year":"2019","journal-title":"Tetrahedron"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.saa.2016.12.009","article-title":"A new unsymmetrical azine derivative based on coumarin group as dual-modal sensor for CN\u2212 and fluorescent \u201cOFF\u2013ON\u201d for Zn2+","volume":"175","author":"Hu","year":"2017","journal-title":"Spectrochim. Acta A"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"93826","DOI":"10.1039\/C6RA17354C","article-title":"Triphenylamine based lab-on-a-molecule for the highly selective and sensitive detection of Zn2+ and CN\u2212 in aqueous solution","volume":"6","author":"Sun","year":"2016","journal-title":"RSC Adv."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107969","DOI":"10.1016\/j.dyepig.2019.107969","article-title":"Terpyridyl-based triphenylamine derivatives with aggregation-induced emission characteristics for selective detection of Zn2+, Cd2+ and CN\u2212 ions and application in cell imaging","volume":"173","author":"Li","year":"2020","journal-title":"Dyes Pigm."},{"key":"ref_38","first-page":"7570","article-title":"Vitamin B6 Cofactor Derivative: A Dual Fluorescent Turn-On Sensor to Detect Zn2+ and CN\u2212 Ions and Its Application in Live Cell Imaging","volume":"2","author":"Anand","year":"2017","journal-title":"Chem. Select"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"126814","DOI":"10.1016\/j.snb.2019.126814","article-title":"Selective chemosensor capable of sensing both CN\u2212 and Zn2+: Its application to zebrafish","volume":"297","author":"Jung","year":"2019","journal-title":"Sens. Actuators B"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1016\/j.tet.2017.03.069","article-title":"Thiophene and diethylaminophenol-based \u201cturn-on\u201d fluorescence chemosensor for detection of Al3+ and F- in a near-perfect aqueous solution","volume":"73","author":"Jeong","year":"2017","journal-title":"Tetrahedron"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5685","DOI":"10.1021\/jo200675j","article-title":"Enantioselective Recognition of Mandelic Acid by a 3,6-Dithiophen-2-yl-9 H -carbazole-Based Chiral Fluorescent Bisboronic Acid Sensor","volume":"76","author":"Wu","year":"2011","journal-title":"J. Org. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"61505","DOI":"10.1039\/C6RA10086D","article-title":"A highly sensitive benzimidazole-based chemosensor for the colorimetric detection of Fe(II) and Fe(III) and the fluorometric detection of Zn(II) in aqueous media","volume":"6","author":"Kim","year":"2016","journal-title":"RSC Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.saa.2014.10.060","article-title":"Dual-channel detection of Cu2+ and F\u2212 with a simple Schiff-based colorimetric and fluorescent sensor","volume":"136","author":"Na","year":"2014","journal-title":"Spectrochim. Acta A"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1038\/nprot.2013.087","article-title":"Relative and absolute determination of fluorescence quantum yields of transparent samples","volume":"8","author":"Grabolle","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_45","first-page":"113","article-title":"Formation and stability of inorganic complexes in solution","volume":"9","author":"Job","year":"1928","journal-title":"Ann. Chim. Appl."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2703","DOI":"10.1021\/ja01176a030","article-title":"A Spectrophotometric Investigation of the Interaction of Iodine with Aromatic Hydrocarbons","volume":"71","author":"Benesi","year":"1949","journal-title":"J. Am. Chem. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4415","DOI":"10.1039\/c2an35752f","article-title":"A turn-on and reversible fluorescence sensor for zinc ion","volume":"137","author":"Lin","year":"2012","journal-title":"Analyst"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1016\/j.tetlet.2012.08.058","article-title":"A turn-on Schiff base fluorescence sensor for zinc ion","volume":"53","author":"Hsieh","year":"2012","journal-title":"Tetrahedron Lett."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.inoche.2015.11.012","article-title":"Turn-on fluorescent chemosensor for selective detection of Zn2+ in an aqueous solution: Experimental and theoretical studies","volume":"63","author":"Choi","year":"2016","journal-title":"Inorg. Chem. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.saa.2018.09.019","article-title":"An azine-based carbothioamide chemosensor for selective and sensitive turn-on-off sequential detection of Zn(II) and H2PO4\u2212, live cell imaging and INHIBIT logic gate","volume":"207","author":"Purkait","year":"2019","journal-title":"Spectrochim. Acta A"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1021\/es00093a013","article-title":"Chemical associations of lead, cadmium, copper, and zinc in street dusts and roadside soils","volume":"15","author":"Harrison","year":"1981","journal-title":"Environ. Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.snb.2011.10.069","article-title":"Azo dyes featuring with nitrobenzoxadiazole (NBD) unit: A new selective chromogenic and fluorogenic sensor for cyanide ion","volume":"161","author":"Tsui","year":"2012","journal-title":"Sens. Actuators B Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.snb.2015.09.129","article-title":"A dual chemosensor for Zn2+ and Co2+ in aqueous media and living cells: Experimental and theoretical studies","volume":"223","author":"Park","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1039\/C4NJ00191E","article-title":"A single chemosensor for multiple analytes: Fluorogenic detection of Zn2+ and OAc\u2212 ions in aqueous solution, and an application to bioimaging","volume":"38","author":"Park","year":"2014","journal-title":"New J. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1039\/C5NJ02334C","article-title":"A highly selective and sensitive fluorescent turn-on Al3+ chemosensor in aqueous media and living cells: Experimental and theoretical studies","volume":"40","author":"Hwang","year":"2016","journal-title":"New J. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.snb.2012.08.038","article-title":"Azo dye-based latent colorimetric chemodosimeter for the selective detection of cyanides in aqueous bis-tris buffer","volume":"174","author":"Park","year":"2012","journal-title":"Sens. Actuators B"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.aca.2019.03.024","article-title":"A highly selective and sensitive dual-mode sensor for colorimetric and turn-on fluorescent detection of cyanide in water, agro-products and living cells","volume":"1065","author":"Guo","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1039\/C8AY02401D","article-title":"Spectrophotometric and RGB performances of a new tetraphenylcyclopenta-derived Schiff base for the quantification of cyanide ions","volume":"11","author":"Venkatesan","year":"2019","journal-title":"Anal. Methods"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/24\/5458\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:41:21Z","timestamp":1760190081000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/24\/5458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,11]]},"references-count":58,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["s19245458"],"URL":"https:\/\/doi.org\/10.3390\/s19245458","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,11]]}}}