{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T10:22:40Z","timestamp":1772360560585,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2012DFM30040"],"award-info":[{"award-number":["2012DFM30040"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2013YZ0002, 2014YZ0001"],"award-info":[{"award-number":["2013YZ0002, 2014YZ0001"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips\u2019 image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (R2) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing.<\/jats:p>","DOI":"10.3390\/s20010209","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T03:28:53Z","timestamp":1578022133000},"page":"209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing"],"prefix":"10.3390","volume":"20","author":[{"given":"Ruixin","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"}]},{"given":"Huihuang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"}]},{"given":"Jianpeng","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"}]},{"given":"Haiyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"}]},{"given":"Min","family":"Du","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China"},{"name":"Key Lab of Eco-Industrial Green Technology of Fujian Province, Nanping 354300, China"}]},{"given":"Mangi","family":"Vai","sequence":"additional","affiliation":[{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"},{"name":"State Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macau 999078, Macau SAR, China"},{"name":"Department of Electrical and Computer Engineering, Faculty of Science and Technology, University of Macau, Macao 999078, China"}]},{"given":"Siohang","family":"Pun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macau 999078, Macau SAR, China"}]},{"given":"Yueming","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China"},{"name":"Key Lab of Medical Instrumentation &amp; Pharmaceutical Technology of Fujian Province, Fuzhou 350108, China"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1002\/jcla.21704","article-title":"Point-of-care fluorescence immunoassay for cardiac panel biomarkers","volume":"28","author":"Kim","year":"2014","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3655","DOI":"10.1038\/s41598-017-03939-8","article-title":"Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.snb.2017.03.036","article-title":"Sensitive fluorescence immunoassay of alpha-fetoprotein through copper ions modulated growth of quantum dots in-situ","volume":"247","author":"Wang","year":"2017","journal-title":"Sens. Actuator B Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.bios.2016.04.027","article-title":"A fluorescent immunosensor for high-sensitivity cardiac troponin I using a spatially-controlled polymeric, nano-scale tracer to prevent quenching","volume":"83","author":"Seo","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17819","DOI":"10.1039\/C7RA00821J","article-title":"CdTe\/CdS quantum dot-labeled fluorescent immunochromatography test strips for rapid detection of Escherichia coli O157: H7","volume":"7","author":"Yu","year":"2017","journal-title":"RSC Adv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.foodchem.2015.08.109","article-title":"Highly sensitive and simultaneous detection of melamine and aflatoxin M1 in milk products by multiplexed planar waveguide fluorescence immunosensor (MPWFI)","volume":"197","author":"Guo","year":"2016","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.snb.2013.12.058","article-title":"Highly sensitive and rapid detection of melamine in milk products by planar waveguide fluorescence immunosensor (PWFI)","volume":"194","author":"Guo","year":"2014","journal-title":"Sens. Actuator B Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.1038\/srep04572","article-title":"A reusable evanescent wave immunosensor for highly sensitive detection of bisphenol A in water samples","volume":"4","author":"Zhou","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1007\/s00216-006-0549-4","article-title":"A novel enhancement assay for immunochromatographic test strips using gold nanoparticles","volume":"385","author":"Tanaka","year":"2006","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7008","DOI":"10.1021\/ac101405a","article-title":"Rapid and Sensitive Detection of Protein Biomarker Using a Portable Fluorescence Biosensor Based on Quantum Dots and a Lateral Flow Test Strip","volume":"82","author":"Li","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.bios.2011.09.002","article-title":"Quantum dot-based immunochromatography test strip for rapid, quantitative and sensitive detection of alpha fetoprotein","volume":"30","author":"Yang","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.cccn.2003.10.002","article-title":"A rapid, simple measurement of human albumin in whole blood using a fluorescence immunoassay (I)","volume":"339","author":"Choi","year":"2004","journal-title":"Clin. Chim. Acta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1002\/jsfa.6232","article-title":"Development of a new fluorescence immunochromatography strip for detection of chloramphenicol residues in chicken muscles","volume":"93","author":"Bai","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1111\/1750-3841.13654","article-title":"Advantage of Eu3+-Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk","volume":"82","author":"Tang","year":"2017","journal-title":"J. Food Sci."},{"key":"ref_15","first-page":"124","article-title":"Research of Range Adjustment for Fluorescent Immuno-Chromatographic Strip Analyzer","volume":"36","author":"Gao","year":"2017","journal-title":"Chin. J. Biomed. Eng."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.compbiomed.2016.04.005","article-title":"Assessment of advanced glycated end product accumulation in skin using auto fluorescence multispectral imaging","volume":"85","author":"Larsson","year":"2017","journal-title":"Comput. Biol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"E354","DOI":"10.1016\/S1470-2045(19)30317-1","article-title":"Latest developments in molecular tracers for fluorescence image-guided cancer surgery","volume":"20","author":"Hernot","year":"2019","journal-title":"Lancet Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cccn.2005.01.026","article-title":"Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing","volume":"356","author":"Oh","year":"2005","journal-title":"Clin. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Huang, M., Yu, W., and Zhu, D. (2012, January 8\u201310). An Improved Image Segmentation Algorithm Based on the Otsu Method. Proceedings of the 13th ACIS International Conference on Software Engineering, Artificial Intelligence, Networking and Parallel\/Distributed Computing, Kyoto, Japan.","DOI":"10.1109\/SNPD.2012.26"},{"key":"ref_20","first-page":"854","article-title":"A Fast Automatic Exposure Control Method for CMOS Image Sensor","volume":"43","author":"Ge","year":"2010","journal-title":"J. Tianjin Univ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1016\/j.measurement.2012.11.039","article-title":"Study on factors enhancing photobleaching effect of fluorescent dye","volume":"46","author":"Kuang","year":"2013","journal-title":"Measurement"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/1\/209\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:46:49Z","timestamp":1760190409000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/1\/209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,30]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["s20010209"],"URL":"https:\/\/doi.org\/10.3390\/s20010209","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,30]]}}}