{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:27:50Z","timestamp":1764332870746,"version":"build-2065373602"},"reference-count":69,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:00:00Z","timestamp":1582934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (L-Cu2+) based on a benzocoumarin-Cu2+ ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (L-Cu2+) contains a 2:1 stoichiometry structure between fluorescent ligand L and paramagnetic Cu2+. L was found to exclusively bond with Cu2+ ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, L-Cu2+, was successfully used as a chemosensor for the detection of biothiols with a fluorescence \u201cOFF-ON\u201d response modality. Upon the addition of biothiols, the decomplexation of L-Cu2+ led to the liberation of the fluorescent ligand, L, resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that L-Cu2+ possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of L-Cu2+ was then demonstrated by the detection of biothiols in human urine sample.<\/jats:p>","DOI":"10.3390\/s20051331","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T03:13:28Z","timestamp":1583205208000},"page":"1331","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the \u2018Naked\u2013Eye\u2019 Detection of Biothiols in Human Urine"],"prefix":"10.3390","volume":"20","author":[{"given":"Yue","family":"Wang","sequence":"first","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1568-9002","authenticated-orcid":false,"given":"Haibo","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmin","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Kang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingtao","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-824X","authenticated-orcid":false,"given":"Run","family":"Zhang","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4856","DOI":"10.1021\/acs.analchem.5b00377","article-title":"Near-infrared and naked-eye fluorescence probe for direct and highly selective detection of cysteine and its application in living cells","volume":"87","author":"Zhang","year":"2015","journal-title":"Anal. 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