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In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L\u22121 level hence affording a simple, highly sensitive, and selective low cost detection platform.<\/jats:p>","DOI":"10.3390\/s20072017","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T03:58:39Z","timestamp":1586231919000},"page":"2017","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs"],"prefix":"10.3390","volume":"20","author":[{"given":"Chen","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China"}]},{"given":"Jialun","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China"}]},{"given":"Lujian","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0529-5253","authenticated-orcid":false,"given":"Jie","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Liu, Q., Zhou, N., and Mo, Y.-Y. (2013). Oncogenic microRNAs in Cancer. MicroRNA in Cancer, Springer.","DOI":"10.1007\/978-94-007-4655-8_5"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nature10888","article-title":"The microcosmos of cancer","volume":"482","author":"Lujambio","year":"2012","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/nature07511","article-title":"MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts","volume":"456","author":"Thum","year":"2008","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1100\/tsw.2011.11","article-title":"High-resolution northern blot for a reliable analysis of microRNAs and their precursors","volume":"11","author":"Koscianska","year":"2011","journal-title":"Sci. World J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/nature09783","article-title":"Pervasive roles of microRNAs in cardiovascular biology","volume":"469","author":"Small","year":"2011","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1002\/anie.201309388","article-title":"Toehold-initiated rolling circle amplification for visualizing individual microRNAs in situ in single cell","volume":"53","author":"Deng","year":"2014","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_7","first-page":"161","article-title":"The Role of MicroRNAs in Human Diseases","volume":"2","author":"Ardekani","year":"2010","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7140","DOI":"10.1039\/c2ra20487h","article-title":"Amplified electrochemical microRNA biosensor using a hemin-G-quadruplex complex as the sensing element","volume":"2","author":"Zhou","year":"2012","journal-title":"RSC Adv."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.snb.2018.01.129","article-title":"Dual-mode fluorescence biosensor platform based on T-shaped duplex structure for detection of microRNA and folate receptor","volume":"261","author":"Tian","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16518","DOI":"10.1039\/C5CC07340E","article-title":"Real-time monitoring of rolling circle amplification using aggregation-induced emission: Applications in biological detection","volume":"51","author":"Jiang","year":"2015","journal-title":"Chem. 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Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.aca.2016.09.019","article-title":"G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection","volume":"943","author":"Jiang","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1021\/ac901794w","article-title":"Universal Aptameric System for Highly Sensitive Detection of Protein Based on Structure-Switching-Triggered Rolling Circle Amplification","volume":"82","author":"Wu","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1039\/c3cs60439j","article-title":"Rolling circle amplification: A versatile tool for chemical biology, materials science and medicine","volume":"43","author":"Ali","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1021\/ac403741y","article-title":"A highly sensitive target-primed rolling circle amplification (TPRCA) method for fluorescent in situ hybridization detection of microRNA in tumor cells","volume":"86","author":"Ge","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1002\/chem.201705338","article-title":"DNAzyme Feedback Amplification: Relaying Molecular Recognition to Exponential DNA Amplification","volume":"24","author":"Liu","year":"2018","journal-title":"Chemistry"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1007\/s00604-017-2450-6","article-title":"Fluorometric determination of microRNA based on strand displacement amplification and rolling circle amplification","volume":"184","author":"Zhou","year":"2017","journal-title":"Microchim. Acta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6896","DOI":"10.1021\/ja0712625","article-title":"Metal-Dependent Global Folding and Activity of the 8-17 DNAzyme Studied by Fluorescence Resonance Energy Transfer","volume":"129","author":"Kim","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.1002\/anie.200800960","article-title":"A highly selective DNAzyme sensor for mercuric ions","volume":"47","author":"Hollenstein","year":"2008","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7664","DOI":"10.1021\/ac300616z","article-title":"DNAzyme-based rolling-circle amplification DNA machine for ultrasensitive analysis of microRNA in Drosophila larva","volume":"84","author":"Wen","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3268","DOI":"10.1002\/anie.200805665","article-title":"Highly sensitive determination of microRNA using target-primed and branched rolling-circle amplification","volume":"48","author":"Cheng","year":"2009","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.snb.2017.12.203","article-title":"Ultrasensitive, label-free detection of T4 ligase and T4 polynucleotide kinase based on target-triggered hyper-branched rolling circle amplification","volume":"260","author":"Jiang","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.bios.2015.04.003","article-title":"RNA-regulated molecular tweezers for sensitive fluorescent detection of microRNA from cancer cells","volume":"71","author":"Gong","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5840","DOI":"10.1039\/c2nr31497e","article-title":"Graphene oxide for rapid microRNA detection","volume":"4","author":"Lu","year":"2012","journal-title":"Nanoscale"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1021\/ac303772m","article-title":"Fluorescence Quenching of Graphene Oxide Integrating with the Site-Specific Cleavage of the Endonuclease for Sensitive and Selective MicroRNA Detection","volume":"85","author":"Tu","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/chem.201203344","article-title":"Sensitive and Convenient Detection of microRNAs Based on Cascade Amplification by Catalytic DNAzymes","volume":"19","author":"Tian","year":"2013","journal-title":"Chem. A Eur. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10822","DOI":"10.1021\/acs.analchem.5b01930","article-title":"Label-Free MicroRNA Detection Based on Fluorescence Quenching of Gold Nanoparticles with a Competitive Hybridization","volume":"21","author":"Wang","year":"2015","journal-title":"Anal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/7\/2017\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:15:19Z","timestamp":1760174119000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/7\/2017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,3]]},"references-count":29,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["s20072017"],"URL":"https:\/\/doi.org\/10.3390\/s20072017","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,3]]}}}