{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T00:21:50Z","timestamp":1775262110135,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Government in the framework of PRIN 2017","award":["2017YER72 K_005"],"award-info":[{"award-number":["2017YER72 K_005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of \u03b2-lactoglobulin (\u03b2-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-\u03b2-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains \u03b2-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of \u03b2-LG within the range 0.1\u201310 ng\u00b7mL\u22121, with a limit of detection of 0.09 ng\u00b7mL\u22121. The biosensor was satisfactorily employed for the determination of spiked \u03b2-LG in real food samples.<\/jats:p>","DOI":"10.3390\/s20082349","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T04:49:38Z","timestamp":1587444578000},"page":"2349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Folding-Based Electrochemical Aptasensor for the Determination of \u03b2-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3917-4713","authenticated-orcid":false,"given":"Olaya","family":"Amor-Guti\u00e9rrez","sequence":"first","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"},{"name":"NanoBioAnalysis Group, Department of Physical and Analytical Chemistry, University of Oviedo, Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9083-074X","authenticated-orcid":false,"given":"Giulia","family":"Selvolini","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3782-5025","authenticated-orcid":false,"given":"M. Teresa","family":"Fern\u00e1ndez-Abedul","sequence":"additional","affiliation":[{"name":"BioNanoAnalytical Spectrometry and Electrochemistry Group, Department of Physical and Analytical Chemistry, University of Oviedo, Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9600-0253","authenticated-orcid":false,"given":"Alfredo","family":"de la Escosura-Mu\u00f1iz","sequence":"additional","affiliation":[{"name":"NanoBioAnalysis Group, Department of Physical and Analytical Chemistry, University of Oviedo, Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2465-7552","authenticated-orcid":false,"given":"Giovanna","family":"Marrazza","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.foodcont.2019.03.017","article-title":"A rapid immobilized trypsin digestion combined with liquid chromatography\u2014Tandem mass spectrometry for the detection of milk allergens in baked food","volume":"102","author":"Qi","year":"2019","journal-title":"Food Control"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5353","DOI":"10.1007\/s00216-018-0989-7","article-title":"Consumer-friendly food allergen detection: Moving towards smartphone-based immunoassays","volume":"410","author":"Ross","year":"2018","journal-title":"Anal. 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