{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T07:20:41Z","timestamp":1781248841572,"version":"3.54.1"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T00:00:00Z","timestamp":1589155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development of China","doi-asserted-by":"publisher","award":["2016YFD0500706"],"award-info":[{"award-number":["2016YFD0500706"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Walmart Foundation","award":["SA1703161"],"award-info":[{"award-number":["SA1703161"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Salmonella is a main cause of foodborne illnesses and rapid screening of Salmonella is the key to prevent Salmonella outbreaks, however available detection methods either require a long time, or need complex pretreatment, or have low sensitivity. In this study, a microfluidic biosensor was developed for Salmonella detection using viscoelastic inertial microfluidics for separating magnetic bacteria from unbound magnetic nanoparticles (MNPs) and enzyme catalytic colorimetry for amplifying biological signals. The polyclonal antibodies and horseradish peroxidase (HRP) modified MNPs were first used to specifically capture Salmonella to form magnetic HRP-bacteria. Both magnetic HRP-bacteria and unbound MNPs were magnetically separated from background and resuspended in viscoelastic polyvinylpyrrolidone solution as sample flow. When sample flow was injected with polyvinylpyrrolidone sheath flow into a T-shaped microchannel, larger-sized magnetic HRP-bacteria could penetrate the sample flow, however smaller-sized MNPs remained in the sample flow due to weaker inertial lift force and elastic lift force, resulting in continuous-flow separation of magnetic HRP-bacteria. Finally, magnetic HRP-bacteria were collected and concentrated to catalyze tetramethyl benzidine, and absorbance was measured to determine the bacteria. This biosensor was able to detect Salmonella as low as 30 CFU\/mL in 1 h and featured the advantages of shorter time due to a one-step immunoreaction, easier extension due to only one antibody and one label, and lower cost due to less expensive materials.<\/jats:p>","DOI":"10.3390\/s20092738","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T12:26:30Z","timestamp":1589199990000},"page":"2738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics"],"prefix":"10.3390","volume":"20","author":[{"given":"Lan","family":"Yao","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaozhe","family":"Cai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-1077","authenticated-orcid":false,"given":"Jianhan","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.bios.2015.07.016","article-title":"A microfluidic droplet digital PCR for simultaneous detection of pathogenic Escherichia coli O157 and Listeria monocytogenes","volume":"74","author":"Bian","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.foodcont.2015.12.022","article-title":"Development of a novel target-enriched multiplex PCR (Tem-PCR) assay for simultaneous detection of five foodborne pathogens","volume":"64","author":"Xu","year":"2016","journal-title":"Food Control"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.foodcont.2015.06.027","article-title":"Survey of five food-borne pathogens in commercial cold food dishes and their detection by multiplex PCR","volume":"59","author":"Yu","year":"2016","journal-title":"Food Control"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.bios.2017.04.044","article-title":"A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability","volume":"96","author":"Liu","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.bjm.2016.04.033","article-title":"Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis","volume":"48","author":"Mirhosseini","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.foodcont.2017.03.042","article-title":"A sandwich-type ELISA for the detection of Listeria monocytogenes using the well-oriented single chain Fv antibody fragment","volume":"79","author":"Liu","year":"2017","journal-title":"Food Control"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.1021\/acsnano.5b00240","article-title":"One-step detection of pathogens and viruses: Combining magnetic relaxation switching and magnetic separation","volume":"9","author":"Chen","year":"2015","journal-title":"ACS Nano"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.bios.2016.07.106","article-title":"One-step multiplexed detection of foodborne pathogens: Combining a quantum dot-mediated reverse assaying strategy and magnetic separation","volume":"86","author":"Yin","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.snb.2018.05.146","article-title":"Magnetic activated cell sorting (MACS) pipette tip for immunomagnetic bacteria separation","volume":"272","author":"Oh","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Park, J.Y., Park, K., Ok, G., Chang, H.J., Park, T.J., Choi, S.W., and Lim, M.C. (2020). Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay. Sensors, 20.","DOI":"10.3390\/s20051395"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.bios.2017.10.005","article-title":"A sensitive biosensor using double-layer capillary based immunomagnetic separation and invertase-nanocluster based signal amplification for rapid detection of foodborne pathogen","volume":"100","author":"Huang","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.bios.2018.11.032","article-title":"Microfluidic chip coupled with optical biosensors for simultaneous detection of multiple analytes: A review","volume":"126","author":"Liao","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1007\/s00216-013-7497-6","article-title":"Rapid colorimetric detection of Salmonella typhimuriumusing a selective filtration technique combined with antibody\u2013magnetic nanoparticle nanocomposites","volume":"406","author":"Shim","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7717","DOI":"10.1038\/srep07717","article-title":"3D-printed microfluidic device for the detection of pathogenic bacteria using size-based separation in helical channel with trapezoid cross-section","volume":"5","author":"Lee","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.bios.2017.07.035","article-title":"A colorimetric and electrochemical immunosensor for point-of-care detection of enterovirus 71","volume":"99","author":"Hou","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7594","DOI":"10.1021\/ac401717f","article-title":"Magnetophoretic chromatography for the detection of pathogenic bacteria with the naked eye","volume":"85","author":"Kwon","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.aca.2015.04.044","article-title":"Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography","volume":"883","author":"Kwon","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"12303","DOI":"10.1021\/acs.analchem.6b03501","article-title":"Viscoelastic Separation of Particles by Size in Straight Rectangular Microchannels: A Parametric Study for a Refined Understanding","volume":"88","author":"Li","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1021\/acscombsci.5b00180","article-title":"Design of a Microfluidic Chip for Magnetic-Activated Sorting of One-Bead-One-Compound Libraries","volume":"18","author":"Cho","year":"2016","journal-title":"Acs Comb. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.snb.2015.03.038","article-title":"Development of a highly effective multi-stage surface acoustic wave SU-8 microfluidic concentrator","volume":"215","author":"Mu","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.bios.2014.08.071","article-title":"Bio-nanogate controlled enzymatic reaction for virus sensing","volume":"67","author":"Wang","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s10404-014-1395-x","article-title":"Inertia-induced focusing dynamics of microparticles throughout a curved microfluidic channel","volume":"18","author":"Xiang","year":"2014","journal-title":"Microfluid. Nanofluid."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"074106","DOI":"10.1063\/1.4866045","article-title":"Inertial modulation of hydrophoretic cell sorting and focusing","volume":"104","author":"Song","year":"2014","journal-title":"Appl. Phys. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10404-013-1322-6","article-title":"Dean flow focusing and separation of small microspheres within a narrow size range","volume":"17","author":"Johnston","year":"2014","journal-title":"Microfluid. Nanofluid."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"12547","DOI":"10.1021\/acs.analchem.6b04564","article-title":"Sheathless Focusing and Separation of Diverse Nanoparticles in Viscoelastic Solutions with Minimized Shear Thinning","volume":"88","author":"Liu","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3078","DOI":"10.1039\/C7LC00671C","article-title":"Microfluidic co-flow of Newtonian and viscoelastic fluids for high-resolution separation of microparticles","volume":"17","author":"Tian","year":"2017","journal-title":"Lab Chip"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6968","DOI":"10.1021\/acsnano.7b02277","article-title":"Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows","volume":"11","author":"Liu","year":"2017","journal-title":"ACS Nano"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/9\/2738\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:27:42Z","timestamp":1760174862000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/9\/2738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,11]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["s20092738"],"URL":"https:\/\/doi.org\/10.3390\/s20092738","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,11]]}}}