{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:28:51Z","timestamp":1780633731792,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity.<\/jats:p>","DOI":"10.3390\/s20113195","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T03:32:21Z","timestamp":1591327941000},"page":"3195","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8764-8357","authenticated-orcid":false,"given":"Hiroki","family":"Ashiba","sequence":"first","affiliation":[{"name":"Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Oyamada","sequence":"additional","affiliation":[{"name":"Research Institute, Fujimori Kogyo Co., Ltd., 1-10-1 Sachiura, Kanazawa-ku, Yokohama, Kanagawa 236-0003, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Research Institute, Fujimori Kogyo Co., Ltd., 1-10-1 Sachiura, Kanazawa-ku, Yokohama, Kanagawa 236-0003, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Ueno","sequence":"additional","affiliation":[{"name":"C&amp;I Co., Ltd., 2004-1 Tamatori, Tsukuba, Ibaraki 300-3255, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4856-422X","authenticated-orcid":false,"given":"Makoto","family":"Fujimaki","sequence":"additional","affiliation":[{"name":"Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/0019-2791(71)90454-X","article-title":"Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G","volume":"8","author":"Engvall","year":"1971","journal-title":"Immunochemistry"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1136\/jcp.31.6.507","article-title":"Enzyme immunoassays with special reference to ELISA techniques","volume":"31","author":"Voller","year":"1978","journal-title":"J. Clin. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0022-1759(92)90061-W","article-title":"Enzyme immunoassay techniques an overview","volume":"150","author":"Porstmann","year":"1992","journal-title":"J. Immunol. Methods"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(79)90352-X","article-title":"Chemiluminescence enzyme immunoassay of cortisol using peroxidase as label","volume":"97","author":"Arakawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1093\/clinchem\/37.9.1639","article-title":"Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for Measuring Tumor Markers","volume":"37","author":"Nishizono","year":"1991","journal-title":"Clin. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.aca.2013.09.050","article-title":"Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen","volume":"801","author":"Liu","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/nbt1072","article-title":"Microfabricated arrays of femtoliter chambers allow single molecule enzymology","volume":"23","author":"Rondelez","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/nbt.1641","article-title":"Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations","volume":"28","author":"Rissin","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1177\/2211068215589580","article-title":"The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing","volume":"21","author":"Wilson","year":"2016","journal-title":"J. Lab. Autom."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1002\/bab.1238","article-title":"A simple, rapid one-step ELISA using antibody-antibody complex","volume":"62","author":"Jiang","year":"2015","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1039\/C6LC01121G","article-title":"One-step immunoassay of C-reactive protein using droplet microfluidics","volume":"16","author":"Tang","year":"2016","journal-title":"Lab Chip"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bios.2016.04.046","article-title":"Portable, one-step, and rapid GMR biosensor platform with smartphone interface","volume":"85","author":"Choi","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1039\/C7AN01066D","article-title":"Single-step homogeneous immunoassay for detecting prostate-specific antigen using dual-color light scattering of metal nanoparticles","volume":"142","author":"Vial","year":"2017","journal-title":"Analyst"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1021\/acssensors.0c00064","article-title":"One-Step Digital Immunoassay for Rapid and Sensitive Detection of Cardiac Troponin I","volume":"5","author":"Wang","year":"2020","journal-title":"ACS Sensors"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3535","DOI":"10.1021\/acs.analchem.9b02526","article-title":"Wash-Free, Digital Immunoassay in Polydisperse Droplets","volume":"92","author":"Byrnes","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0250-6874(82)80008-5","article-title":"Gas detection by means of surface plasmon resonance","volume":"3","author":"Nylander","year":"1982","journal-title":"Sens. Actuators"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0925-4005(98)00321-9","article-title":"Surface plasmon resonance sensors: Review","volume":"54","author":"Homola","year":"1999","journal-title":"Sens. Actuators B"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1364\/OL.27.000512","article-title":"Sensor based on an integrated optical microcavity","volume":"27","author":"Krioukov","year":"2002","journal-title":"Opt. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1063\/1.1605261","article-title":"Biochemical sensors based on polymer microrings with sharp asymmetrical resonance","volume":"83","author":"Chao","year":"2003","journal-title":"Appl. Phys. Lett."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"20701","DOI":"10.1073\/pnas.0808988106","article-title":"Single virus detection from the reactive shift of a whispering-gallery mode","volume":"105","author":"Vollmer","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6408","DOI":"10.1364\/OE.16.006408","article-title":"Silica-based monolithic sensing plates for waveguide-mode sensors","volume":"16","author":"Fujimaki","year":"2008","journal-title":"Opt. Express"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"15732","DOI":"10.1364\/OE.18.015732","article-title":"Detection of colored nanomaterials using evanescent field-based waveguide sensors","volume":"18","author":"Fujimaki","year":"2010","journal-title":"Opt. Express"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Gopinath, S.C.B., Awazu, K., Fujimaki, M., Shimizu, K., and Shima, T. (2013). Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors. PLoS ONE, 8.","DOI":"10.1371\/annotation\/7bb0ff7b-3527-4a42-a50a-ec81f108ac41"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10925","DOI":"10.1364\/OE.23.010925","article-title":"Parallel-incidence-type waveguide-mode sensor with spectral-readout setup","volume":"23","author":"Fujimaki","year":"2015","journal-title":"Opt. Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0161-5890(01)00042-6","article-title":"Human C-reactive protein: Expression, structure, and function","volume":"38","author":"Volanakis","year":"2001","journal-title":"Mol. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1016\/j.bios.2005.09.010","article-title":"SPR-based immunosensor for the CRP detection\u2014A new method to detect a well known protein","volume":"21","author":"Meyer","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.snb.2012.03.083","article-title":"Development of a disposable potentiometric antibody immobilized ZnO nanotubes based sensor for the detection of C-reactive protein","volume":"166\u2013167","author":"Ibupoto","year":"2012","journal-title":"Sens. Actuators B Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.snb.2013.08.078","article-title":"Label-free CRP detection using optical biosensor with one-step immobilization of antibody on nitrocellulose membrane","volume":"190","author":"Kim","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.bioelechem.2015.10.002","article-title":"Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor","volume":"107","author":"Yagati","year":"2016","journal-title":"Bioelectrochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17131","DOI":"10.1038\/s41598-019-52285-4","article-title":"A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein","volume":"9","author":"Chuang","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_31","unstructured":"(2020, May 22). Human CRP ELISA Kit, LCL Inc. Available online: http:\/\/www.icllab.com\/elisa-kits\/human-crp-elisa-kit.html."},{"key":"ref_32","unstructured":"(2020, May 22). Human C Reactive Protein ELISA Kit, Abcam Plc. Available online: https:\/\/www.abcam.com\/human-c-reactive-protein-elisa-kit-crp-ab99995.html."},{"key":"ref_33","unstructured":"(2020, May 22). CRP (human) ELISA kit, Enzo Life Sciences, Inc. Available online: http:\/\/www.enzolifesciences.com\/ENZ-KIT102\/crp-human-elisa-kit."},{"key":"ref_34","unstructured":"(2020, May 22). Human C-Reactive Protein\/CRP Quantikine ELISA Kit, R&D Systems, Inc. Available online: https:\/\/www.rndsystems.com\/products\/human-c-reactive-protein-crp-quantikine-elisa-kit_dcrp00."},{"key":"ref_35","unstructured":"(2020, May 22). CircuLex High-Sensitivity CRP ELISA Kit, Medical & Biological Laboratories Co., Ltd. Available online: https:\/\/ruo.mbl.co.jp\/bio\/e\/dtl\/P\/?pcd=CY-8071."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2869","DOI":"10.1007\/s00216-012-6308-9","article-title":"Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma","volume":"404","author":"Homola","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.bios.2013.02.004","article-title":"Aptamer\/thrombin\/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin","volume":"47","author":"Bai","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_38","unstructured":"Born, M., and Wolf, E. (1980). Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light, Pergamon. [6th ed.]. Chapter 1."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1364\/JOSA.55.001205","article-title":"Interspecimen Comparison of the Refractive Index of Fused Silica","volume":"55","author":"Malitson","year":"1965","journal-title":"J. Opt. Soc. Am."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"067168","DOI":"10.1063\/1.4923379","article-title":"Uncertainty analysis for the coefficient of band-to-band absorption of crystalline silicon","volume":"5","author":"Schinke","year":"2015","journal-title":"AIP Adv."},{"key":"ref_41","unstructured":"Parik, E.D. (1998). Water (H2O). Handbook of Optical Constants of Solids II, Academic Press."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1364\/AO.54.000477","article-title":"Optical constants of Cu, Ag, and Au revisited","volume":"54","author":"Babar","year":"2015","journal-title":"Appl. Opt."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/11\/3195\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:35:46Z","timestamp":1760175346000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/11\/3195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,4]]},"references-count":42,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["s20113195"],"URL":"https:\/\/doi.org\/10.3390\/s20113195","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,4]]}}}