{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T01:37:55Z","timestamp":1772761075946,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T00:00:00Z","timestamp":1591401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007655","name":"\u010cesk\u00e9 Vysok\u00e9 U\u010den\u00ed Technick\u00e9 v Praze","doi-asserted-by":"publisher","award":["SGS17\/110\/OHK4\/1T\/17"],"award-info":[{"award-number":["SGS17\/110\/OHK4\/1T\/17"]}],"id":[{"id":"10.13039\/100007655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007655","name":"\u010cesk\u00e9 Vysok\u00e9 U\u010den\u00ed Technick\u00e9 v Praze","doi-asserted-by":"publisher","award":["SGS18\/097\/OHK4\/1T\/17"],"award-info":[{"award-number":["SGS18\/097\/OHK4\/1T\/17"]}],"id":[{"id":"10.13039\/100007655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007435"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007435"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified with (3-aminopropyl)triethoxysilane (APTES). The repeatability of the preparation of the optical probe and its use was demonstrated with five differently shaped OFEs. The intensity of measured bioluminescence was minimally influenced by the OFE shape, possessing transmittances between 1.41% and 5.00%. OFE probes layered with P. putida TVA8 were used to monitor liquid toluene over a two-week period. It was demonstrated that OFE probes layered with positively induced P. putida TVA8 bioreporters were reliable detectors of toluene. A toluene concentration of 26.5 mg\/L was detected after &lt;30 min after immersion of the probe in the toluene solution. Additional experiments also immobilized constitutively bioluminescent cells of E. coli 652T7, on OFEs with polyethyleneimine (PEI). These OFEs were repetitively induced with Lauria-Bertani (LB) nutrient medium. Bioluminescence appeared 15 minutes after immersion of the OFE in LB. A change in pH from 7 to 6 resulted in a decrease in bioluminescence that was not restored following additional nutrient inductions at pH 7. The E. coli 652T7 OFE probe was therefore sensitive to negative influences but could not be repetitively used.<\/jats:p>","DOI":"10.3390\/s20113237","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T05:16:14Z","timestamp":1591679774000},"page":"3237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Repetitive Detection of Aromatic Hydrocarbon Contaminants with Bioluminescent Bioreporters Attached on Tapered Optical Fiber Elements"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3997-6452","authenticated-orcid":false,"given":"Jakub","family":"Zaj\u00edc","sequence":"first","affiliation":[{"name":"Faculty of Biomedical Engineering, Czech Technical University in Prague, 27201 Kladno, Czech Republic"},{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA"},{"name":"Department of Cardiology, Regional Hospital Liberec, 46063 Liberec, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-1764","authenticated-orcid":false,"given":"Steven","family":"Ripp","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0369-5082","authenticated-orcid":false,"given":"Josef","family":"Tr\u00f6gl","sequence":"additional","affiliation":[{"name":"Faculty of Environment, Jan Evangelista Purkyn\u011b University in \u00dast\u00ed nad Labem, 40096 \u00dast\u00ed nad Labem, Czech Republic"}]},{"given":"Gabriela","family":"Kuncov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Environment, Jan Evangelista Purkyn\u011b University in \u00dast\u00ed nad Labem, 40096 \u00dast\u00ed nad Labem, Czech Republic"},{"name":"Institute of Chemical Process Fundamentals of the ASCR, 16502 Prague, Czech Republic"}]},{"given":"Marie","family":"Posp\u00ed\u0161ilov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Biomedical Engineering, Czech Technical University in Prague, 27201 Kladno, Czech Republic"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,6]]},"reference":[{"key":"ref_1","unstructured":"Jones, O., Preston, M.R., Fawell, J., Mayes, W., Cartmell, E., Pollard, S., Harrison, R.M., Mackenzie, A.R., Williams, M., and Maynard, R. 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