{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T10:31:49Z","timestamp":1780482709417,"version":"3.54.1"},"reference-count":40,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T00:00:00Z","timestamp":1604707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073602"],"award-info":[{"award-number":["82073602"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Norman Bethune Program of Jilin University","award":["2015228"],"award-info":[{"award-number":["2015228"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Component analysis plays an important role in food production, pharmaceutics and agriculture. Nanozymes have attracted wide attention in analytical applications for their enzyme-like properties. In this work, a fluorometric method is described for the determination of thiamine (TH) (vitamin B1) based on hemoglobin\u2013Cu3(PO4)2 nanoflowers (Hb\u2013Cu3(PO4)2 NFs) with peroxidase-like properties. The Hb\u2013Cu3(PO4)2 NFs catalyzed the decomposition of H2O2 into \u00b7OH radicals in an alkaline solution that could efficiently react with nonfluorescent thiamine to fluoresce thiochrome. The fluorescence of thiochrome was further enhanced with a nonionic surfactant, Tween 80. Under optimal reaction conditions, the linear range for thiamine was from 5 \u00d7 10\u22128 to 5 \u00d7 10\u22125 mol\/L. The correlation coefficient for the calibration curve and the limit of detection (LOD) were 0.9972 and 4.8 \u00d7 10\u22128 mol\/L, respectively. The other vitamins did not bring about any obvious changes in fluorescence. The developed method based on hybrid nanoflowers is specific, pragmatically simple and sensitive, and has potential for application in thiamine detection.<\/jats:p>","DOI":"10.3390\/s20216359","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T19:03:37Z","timestamp":1604862217000},"page":"6359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fluorometric Detection of Thiamine Based on Hemoglobin\u2013Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity"],"prefix":"10.3390","volume":"20","author":[{"given":"Hangjin","family":"Zou","sequence":"first","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongtian","family":"Sheng","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3093-6964","authenticated-orcid":false,"given":"Yanfei","family":"Qi","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin University, Changchun 130021, Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.colsurfb.2011.07.051","article-title":"Simultaneous determination of uric acid, xanthine and hypoxanthine at poly(pyrocatechol violet)\/functionalized multi-walled carbon nanotubes composite film modified electrode","volume":"88","author":"Wang","year":"2011","journal-title":"Colloid Surf. 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