{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T06:28:25Z","timestamp":1769236105393,"version":"3.49.0"},"reference-count":30,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772068"],"award-info":[{"award-number":["31772068"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"undefined  &lt;span style=&quot;color:gray;font-size:10px;&quot;&gt;undefined&lt;\/span&gt;","award":["2018CXGC0214"],"award-info":[{"award-number":["2018CXGC0214"]}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["2018ZBXC323"],"award-info":[{"award-number":["2018ZBXC323"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2018ZC0126"],"award-info":[{"award-number":["ZR2018ZC0126"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Immunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost field detection technology. However, a traditional TRFIA paper-based sensor is based on antibody labeling, which cannot easily meet the current detection requirements. A second antibody labeling method was used to amplify the fluorescence signal and improve the detection sensitivity. Polystyrene fluorescent microspheres were combined with sheep anti-mouse IgG to prepare fluorescent probes (Eu-IgGs). After the probe fully reacted with the antibody (Eu-IgGs-Abs) in the sample cell, it was deployed on the paper-based sensor using chromatography. Eu-IgGs-Abs that were not bound to the target were captured on the T-line, while those that were bound were captured on the C-line. The paper-based sensor reflected the corresponding fluorescence intensity change. Because a single molecule of the deoxynivalenol antibody could bind to multiple Eu-IgGs, this method could amplify the fluorescence signal intensity on the unit antibody and improve the detection sensitivity. The working standard curve of the sensor was established under the optimum working conditions. It showed the lower limit of detection and higher recovery rate when it was applied to actual samples and compared with other methods. This sensor has the advantages of high sensitivity, good accuracy, and good specificity, saving the amount of antibody consumed and being suitable for rapid field detection of deoxynivalenol.<\/jats:p>","DOI":"10.3390\/s20226577","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T07:41:00Z","timestamp":1605685260000},"page":"6577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol"],"prefix":"10.3390","volume":"20","author":[{"given":"Haowei","family":"Dong","sequence":"first","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]},{"given":"Xingshuang","family":"An","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"College of Life Science, Yantai University, No. 30 Qingquan Road, Yantai 264005, China"}]},{"given":"Yaodong","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]},{"given":"Fukai","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China"}]},{"given":"Qingqing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]},{"given":"Xia","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]},{"given":"Yemin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.biocontrol.2016.10.004","article-title":"Crop molds and mycotoxins: Alternative management using biocontrol","volume":"104","author":"Nguyen","year":"2017","journal-title":"Biol. Control."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1039\/C9NJ04804A","article-title":"An electrochemical immunosensor based on a combined amplification strategy with the GO-CS\/CeO2-CS nanocomposite for the detection of aflatoxin M1","volume":"44","author":"An","year":"2020","journal-title":"New J. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.trac.2018.02.007","article-title":"Recent progress in application of nanomaterial enabled-biosensors for ochratoxin a detection","volume":"102","author":"Jiang","year":"2018","journal-title":"Trac-Trend Anal. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"121735","DOI":"10.1016\/j.jchromb.2019.121735","article-title":"Identification and determination of deoxynivalenol (DON) and deepoxy-deoxynivalenol (DOM-1) in pig colostrum and serum using liquid chromatography in combination with high resolution mass spectrometry (LC-MS\/MS (HR))","volume":"1126\u20131127","author":"Stastny","year":"2019","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"123379","DOI":"10.1016\/j.jhazmat.2020.123379","article-title":"Advances in nanomaterial-based electrochemical biosensors for the detection of microbial toxins, pathogenic bacteria in food matrices","volume":"401","author":"Gupta","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111676","DOI":"10.1016\/j.fct.2020.111676","article-title":"The neurotoxicity of trichothecenes T-2 toxin and deoxynivalenol (DON): Current status and future perspectives","volume":"145","author":"Zhang","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"111649","DOI":"10.1016\/j.fct.2020.111649","article-title":"The biological detoxification of deoxynivalenol: A review","volume":"145","author":"Yao","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.micpath.2018.06.045","article-title":"Determination of mycotoxins by HPLC, LC-ESI-MS\/MS, and MALDI-TOF MS in Fusarium species-infected sugarcane","volume":"123","author":"Zhang","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.foodchem.2017.11.040","article-title":"Determination of multiple mycotoxins in feedstuffs by combined use of UPLC-MS\/MS and UPLC-QTOF-MS","volume":"267","author":"Romera","year":"2018","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100058","DOI":"10.1016\/j.toxcx.2020.100058","article-title":"Quantification of zearalenone and \u03b1-zearalenol in swine liver and reproductive tissues using GC-MS","volume":"8","author":"Pack","year":"2020","journal-title":"Toxicon X"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"127888","DOI":"10.1016\/j.chemosphere.2020.127888","article-title":"Suspect screening of natural toxins in surface and drinking water by high performance liquid chromatography and high-resolution mass spectrometry","volume":"261","author":"Picardo","year":"2020","journal-title":"Chemosphere"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhang, H. (2019). Ultrasensitive Immunosensor for Aflatoxin B1 Detection Based on Screen-Printed Carbon Electrode Modified by Ferrocene @Multi-Walled Carbon Nanotubes. Int. J. Electrochem. Sci., 9170\u20139180.","DOI":"10.20964\/2019.09.61"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1039\/b817836d","article-title":"A regenerable immunochip for the rapid determination of 13 different antibiotics in raw milk","volume":"134","author":"Kloth","year":"2009","journal-title":"Analyst"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.bios.2016.09.041","article-title":"A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples","volume":"87","author":"Soares","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s00604-019-3570-y","article-title":"A \u2018turnon\u2019 aptasensor for simultaneous and time-resolved fluorometric determination of zearalenone, trichothecenes A and aflatoxin B1 using WS2 as a quencher","volume":"186","author":"Niazi","year":"2019","journal-title":"Mikrochim. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"133793","DOI":"10.1016\/j.scitotenv.2019.133793","article-title":"Dual-label time-resolved fluoroimmunoassay as an advantageous approach for investigation of diethyl phthalate & dibutyl phthalate in surface water","volume":"695","author":"Zhu","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.snb.2019.01.149","article-title":"Simultaneous detection of gastric cancer screening biomarkers plasma pepsinogen I\/II using fluorescent immunochromatographic strip coupled with a miniature analytical device","volume":"286","author":"Li","year":"2019","journal-title":"Sens. Actuators B Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1039\/C5AY00100E","article-title":"Monoclonal antibody-europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed","volume":"7","author":"Zhang","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_19","first-page":"36","article-title":"Development and performance of a fast quantitative test strip for vomiting toxin based on time-resolved fluorescent nanometer microspheres","volume":"42","author":"Xiao","year":"2017","journal-title":"Food Process."},{"key":"ref_20","first-page":"3490","article-title":"Applicability evaluation of time-resolved fluoro-immunoassay detection system for deoxynivalenol","volume":"9","author":"Wang","year":"2018","journal-title":"J. Food Saf. Qual."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.foodchem.2018.09.112","article-title":"Development of a new format of competitive immunochromatographic assay using secondary antibody-europium nanoparticle conjugates for ultrasensitive and quantitative determination of ochratoxin A","volume":"275","author":"Majdinasab","year":"2019","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5671","DOI":"10.1021\/acs.jafc.8b01016","article-title":"Rapid, On-Site, Ultrasensitive Melamine Quantitation Method for Protein Beverages Using Time-Resolved Fluorescence Detection Paper","volume":"66","author":"Li","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"120865","DOI":"10.1016\/j.talanta.2020.120865","article-title":"EuNPs-mAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of porcine epidemic diarrhea virus","volume":"214","author":"Xu","year":"2020","journal-title":"Talanta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.foodchem.2018.07.075","article-title":"Ultrasensitive and eco-friendly immunoassays based monoclonal antibody for detection of deoxynivalenol in cereal and feed samples","volume":"270","author":"Kong","year":"2019","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"125176","DOI":"10.1016\/j.foodchem.2019.125176","article-title":"Simultaneous detection of aflatoxin B1, ochratoxin A, zearalenone and deoxynivalenol in corn and wheat using surface plasmon resonance","volume":"300","author":"Wei","year":"2019","journal-title":"Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.foodchem.2018.06.096","article-title":"A peptide\/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed","volume":"268","author":"Xu","year":"2018","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1007\/s12161-018-1198-x","article-title":"One-Step Core\/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize","volume":"11","author":"Zhang","year":"2018","journal-title":"Food Anal. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"112895","DOI":"10.1016\/j.jpba.2019.112895","article-title":"Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine","volume":"177","author":"Huang","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"042017","DOI":"10.1088\/1755-1315\/242\/4\/042017","article-title":"Quantitative detection of Deoxynivalenol in food and feed based on nanogold immunochromatographic assay","volume":"242","author":"Jin","year":"2019","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1080\/19440049.2019.1606454","article-title":"Simple validated method for simultaneous determination of deoxynivalenol, nivalenol, and their 3-\u03b2-D-glucosides in baby formula and Korean rice wine via HPLC-UV with immunoaffinity cleanup","volume":"36","author":"Lee","year":"2019","journal-title":"Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/22\/6577\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:33:34Z","timestamp":1760178814000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/20\/22\/6577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,18]]},"references-count":30,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["s20226577"],"URL":"https:\/\/doi.org\/10.3390\/s20226577","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,18]]}}}