{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:43:21Z","timestamp":1760233401986,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007435"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007435"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic\u2013inorganic polymer ORMOCER\u00ae. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of Saccharomyces cerevisiae. Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (\u22652 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.<\/jats:p>","DOI":"10.3390\/s21020565","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T01:33:29Z","timestamp":1610674409000},"page":"565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor"],"prefix":"10.3390","volume":"21","author":[{"given":"Lucie","family":"Ko\u0161tejnov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojov\u00e1 135\/1, 16502 Prague, Czech Republic"}]},{"given":"Jakub","family":"Ondr\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojov\u00e1 135\/1, 16502 Prague, Czech Republic"}]},{"given":"Petra","family":"Majerov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojov\u00e1 135\/1, 16502 Prague, Czech Republic"}]},{"given":"Martin","family":"Ko\u0161tejn","sequence":"additional","affiliation":[{"name":"Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojov\u00e1 135\/1, 16502 Prague, Czech Republic"}]},{"given":"Gabriela","family":"Kuncov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojov\u00e1 135\/1, 16502 Prague, Czech Republic"},{"name":"Faculty of Environment, Jan Evangelista Purkyn\u011b University in \u00dast\u00ed nad Labem, Pasteurova 3632\/15, 40096 \u00dast\u00ed nad Labem, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0369-5082","authenticated-orcid":false,"given":"Josef","family":"Tr\u00f6gl","sequence":"additional","affiliation":[{"name":"Faculty of Environment, Jan Evangelista Purkyn\u011b University in \u00dast\u00ed nad Labem, Pasteurova 3632\/15, 40096 \u00dast\u00ed nad Labem, Czech Republic"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"unstructured":"(2021, January 06). 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