{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T03:23:55Z","timestamp":1771039435623,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T00:00:00Z","timestamp":1610841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Several studies were devoted to the design of molecularly imprinted polymer (MIP)-based sensors for the detection of a given protein. Here, we bring elements that could contribute to the understanding of the interaction mechanism involved in the recognition of a protein by an imprint. For this purpose, a polydopamine (PDA)-MIP was designed for bovine serum albumin (BSA) recognition. Prior to BSA grafting, the gold surfaces were functionalized with mixed self-assembled monolayers of (MUDA)\/(MHOH) (1\/9, v\/v). The MIP was then elaborated by dopamine electropolymerization and further extraction of BSA templates by incubating the electrode in proteinase K solution. Three complementary techniques, electrochemistry, zetametry, and Fourier-transform infrared spectrometry, were used to investigate pH and ionic strength effects on a MIP\u2019s design and the further recognition process of the analytes by the imprints. Several MIPs were thus designed in acidic, neutral, and basic media and at various ionic strength values. Results indicate that the most appropriate conditions, to achieve a successful MIPs, were an ionic strength of 167 mM and a pH of 7.4. Sensitivity and dissociation constant of the designed sensor were of order of (3.36 \u00b1 0.13) \u00b5A\u00b7cm\u22122\u00b7mg\u22121\u00b7mL and (8.56 \u00b1 6.09) \u00d7 10\u221211 mg\/mL, respectively.<\/jats:p>","DOI":"10.3390\/s21020619","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T03:34:25Z","timestamp":1611113665000},"page":"619","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Contribution to the Understanding of the Interaction between a Polydopamine Molecular Imprint and a Protein Model: Ionic Strength and pH Effect Investigation"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0915-3041","authenticated-orcid":false,"given":"Amal","family":"Tlili","sequence":"first","affiliation":[{"name":"LIMA Laboratory, Faculty of Medicine of Monastir, Monastir University, Av. Avicenne, Monastir 5019, Tunisia"},{"name":"SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France"}]},{"given":"Ghada","family":"Attia","sequence":"additional","affiliation":[{"name":"SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France"}]},{"given":"Sohayb","family":"Khaoulani","sequence":"additional","affiliation":[{"name":"SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France"}]},{"given":"Zouhour","family":"Mazouz","sequence":"additional","affiliation":[{"name":"NANOMISENE Laboratory, CRMN, Technop\u00f4le Sousse, Sousse University, Sousse 4050, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0583-4229","authenticated-orcid":false,"given":"Chouki","family":"Zerrouki","sequence":"additional","affiliation":[{"name":"SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France"}]},{"given":"Nourdin","family":"Yaakoubi","sequence":"additional","affiliation":[{"name":"LAUM Laboratory, Le Mans University, UMR CNR 6613, Avenue Olivier Messiaen, CEDEX 9, 72085 Le Mans, France"}]},{"given":"Ali","family":"Othmane","sequence":"additional","affiliation":[{"name":"LIMA Laboratory, Faculty of Medicine of Monastir, Monastir University, Av. Avicenne, Monastir 5019, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-4411","authenticated-orcid":false,"given":"Najla","family":"Fourati","sequence":"additional","affiliation":[{"name":"SATIE Laboratory, Cnam, UMR CNRS 8029, 292 Rue Saint Martin, 75003 Paris, France"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1093\/chromsci\/bmt024","article-title":"Molecularly imprinted solid-phase extraction in the analysis of agrochemicals","volume":"51","author":"Yi","year":"2013","journal-title":"J. Chromatogr. 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