{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:48:23Z","timestamp":1760233703832,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T00:00:00Z","timestamp":1613174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers \u201cDigital bi-odesign and personalized healthcare\u201d","award":["075-15-2020-913"],"award-info":[{"award-number":["075-15-2020-913"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of \u00absilicon-on-insulator\u00bb (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 \u00d7 10\u221217 M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans.<\/jats:p>","DOI":"10.3390\/s21041333","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T05:54:49Z","timestamp":1613282089000},"page":"1333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Raman Spectroscopy-Based Quality Control of \u201cSilicon-On-Insulator\u201d Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9404-1660","authenticated-orcid":false,"given":"Kristina A.","family":"Malsagova","sequence":"first","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7415-1405","authenticated-orcid":false,"given":"Vladimir P.","family":"Popov","sequence":"additional","affiliation":[{"name":"Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}]},{"given":"Igor N.","family":"Kupriyanov","sequence":"additional","affiliation":[{"name":"Sobolev Institute of Geology and Mineralogy, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}]},{"given":"Tatyana O.","family":"Pleshakova","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Rafael A.","family":"Galiullin","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Andrey F.","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Ivan D.","family":"Shumov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5961-7182","authenticated-orcid":false,"given":"Dmitry I.","family":"Larionov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Fedor V.","family":"Tikhonenko","sequence":"additional","affiliation":[{"name":"Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5119-9449","authenticated-orcid":false,"given":"Svetlana I.","family":"Kapustina","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Vadim S.","family":"Ziborov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"},{"name":"Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia"}]},{"given":"Oleg F.","family":"Petrov","sequence":"additional","affiliation":[{"name":"Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia"}]},{"given":"Olga A.","family":"Gadzhieva","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Institution \u201cN. N. Burdenko National Medical Research Center of Neurosurgery\u201d of the Ministry of Health of the Russian Federation, 125047 Moscow, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5559-2678","authenticated-orcid":false,"given":"Boris A.","family":"Bashiryan","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Institution \u201cN. N. Burdenko National Medical Research Center of Neurosurgery\u201d of the Ministry of Health of the Russian Federation, 125047 Moscow, Russia"}]},{"given":"Vadim N.","family":"Shimansky","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Institution \u201cN. N. Burdenko National Medical Research Center of Neurosurgery\u201d of the Ministry of Health of the Russian Federation, 125047 Moscow, Russia"}]},{"given":"Alexander I.","family":"Archakov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]},{"given":"Yuri D.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Das, N., Dai, Y., Liu, P., Hu, C., Tong, L., Chen, X., and Smith, Z. (2017). Raman Plus X: Biomedical Applications of Multimodal Raman Spectroscopy. Sensors, 17.","DOI":"10.3390\/s17071592"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"do Nascimento, G.M. (2018). Raman Spectroscopy for In Vivo Medical Diagnosis. 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