{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:47:12Z","timestamp":1777434432465,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T00:00:00Z","timestamp":1614124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT)","award":["2017R1A6A3A11035249 and 2020R1C1C1005523"],"award-info":[{"award-number":["2017R1A6A3A11035249 and 2020R1C1C1005523"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The electrochemical-based detection of uric acid (UA) is widely used for diagnostic purposes. However, various interfering species such as ascorbic acid, dopamine, and glucose can affect electrochemical signals, and hence there is an outstanding need to develop improved sensing platforms to detect UA with high selectivity. Herein, we report a pentagonal mediator-based non-enzymatic electrochemical biosensing platform to selectively measure UA in the presence of interfering species. The working electrode was fabricated by electrodepositing polymerized 1-vinylimidazole (PVI), which has an imidazole ligand, onto indium tin oxide (ITO), and then conjugating nickel ions to the PVI-coated ITO electrode. Electrode performance was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements and integrated together with pentacyanoammineferrate, which can bind to the amine groups of UA and function as an electron transferring mediator. The experimental results showed a wide linear range of UA concentration-dependent responses and the multi-potential step (MPS) technique facilitated selective detection of UA in the presence of physiologically relevant interfering species. Altogether, these findings support that pentacyanoammineferrate-based non-enzymatic electrodes are suitable biosensing platforms for the selective measurement of UA, and such approaches could potentially be extended to other bioanalytes as well.<\/jats:p>","DOI":"10.3390\/s21051574","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T02:36:13Z","timestamp":1614220573000},"page":"1574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pentacyanoammineferrate-Based Non-Enzymatic Electrochemical Biosensing Platform for Selective Uric Acid Measurement"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9650-4470","authenticated-orcid":false,"given":"Won-Yong","family":"Jeon","sequence":"first","affiliation":[{"name":"School of Chemical Engineering, Biomedical Institute for Convergence, Sungkyunkwan University, Suwon 16419, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Jun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan, Chungnam 31116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tun Naw","family":"Sut","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, Biomedical Institute for Convergence, Sungkyunkwan University, Suwon 16419, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyug-Han","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan, Chungnam 31116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Bong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan, Chungnam 31116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ijcard.2015.08.109","article-title":"Regulation of uric acid metabolism and excretion","volume":"213","author":"Maiuolo","year":"2016","journal-title":"Int. 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