{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T02:49:27Z","timestamp":1773629367733,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T00:00:00Z","timestamp":1614902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["19-07-00300 ; 19\u201307\u201300304"],"award-info":[{"award-number":["19-07-00300 ; 19\u201307\u201300304"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A method for the rapid detection of coronaviruses is presented on the example of the transmissible gastroenteritis virus (TGEV) directly in aqueous solutions with different conductivity. An acoustic sensor based on a slot wave in an acoustic delay line was used for the research. The addition of anti-TGEV antibodies (Abs) diluted in an aqueous solution led to a change in the depth and frequency of resonant peaks on the frequency dependence of the insertion loss of the sensor. The difference in the output parameters of the sensor before and after the biological interaction of the TGE virus in solutions with the specific antibodies allows drawing a conclusion about the presence\/absence of the studied viruses in the analyzed solution. The possibility for virus detection in aqueous solutions with the conductivity of 1.9\u2013900 \u03bcs\/cm, as well as in the presence of the foreign viral particles, has been demonstrated. The analysis time did not exceed 10 min.<\/jats:p>","DOI":"10.3390\/s21051822","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T11:46:09Z","timestamp":1614944769000},"page":"1822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Acoustical Slot Mode Sensor for the Rapid Coronaviruses Detection"],"prefix":"10.3390","volume":"21","author":[{"given":"Olga","family":"Guliy","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1859-0673","authenticated-orcid":false,"given":"Boris","family":"Zaitsev","sequence":"additional","affiliation":[{"name":"Kotel\u2019nikov Institute of Radio Engineering and Electronics of RAS, Saratov Branch, Saratov 410019, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1743-170X","authenticated-orcid":false,"given":"Andrey","family":"Teplykh","sequence":"additional","affiliation":[{"name":"Kotel\u2019nikov Institute of Radio Engineering and Electronics of RAS, Saratov Branch, Saratov 410019, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Balashov","sequence":"additional","affiliation":[{"name":"Information Technology Center Renato Archer, Campinas CEP, SP 13069-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Fomin","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Staroverov","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Borodina","sequence":"additional","affiliation":[{"name":"Kotel\u2019nikov Institute of Radio Engineering and Electronics of RAS, Saratov Branch, Saratov 410019, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,5]]},"reference":[{"key":"ref_1","unstructured":"Beeching, N.J., Fletcher, T.E., and Fowler, R. (2021, February 26). COVID-19. BMJ Best Practices. BMJ Publishing Group. Available online: http:\/\/bestpractice.bmj.com\/topics\/en-gb\/3000168."},{"key":"ref_2","unstructured":"(2021, February 14). Communicable Disease Threats Report, 9\u201315 February 2020, Week-7. ECDC. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/communicable-disease-threats-report-9-15-february-2020-week-7."},{"key":"ref_3","first-page":"22","article-title":"Investigation on the porcine epidemic diarrhea prevalent on Qinhai","volume":"22","author":"Cheng","year":"1992","journal-title":"Vet. Sci."},{"key":"ref_4","unstructured":"Straw, B.E., D\u2019Allaire, S., Mengeling, W.L., and Taylor, D.J. (2000). Disease of Swine, Iowa State University Press."},{"key":"ref_5","unstructured":"Carte, R.J., and Saunders, V. (2007). Virology: Principles and Applications, John Wiley & Sons Ltd."},{"key":"ref_6","first-page":"hzw010","article-title":"Recent advances in diagnostic testing for viral infections. Biosci Horizons","volume":"9","author":"Souf","year":"2016","journal-title":"Int. J. Stud. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/jmv.20008","article-title":"Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays","volume":"72","author":"Coiras","year":"2004","journal-title":"J. Med. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.virusres.2019.01.013","article-title":"Optimization of qRT-PCR assay for zika virus detection in human serum and urine","volume":"263","author":"Puri","year":"2019","journal-title":"Virus Res."},{"key":"ref_9","first-page":"385","article-title":"Biosensors: Frontiers in rapid detection of COVID-19","volume":"10","author":"Samson","year":"2020","journal-title":"Biotech"},{"key":"ref_10","first-page":"2277","article-title":"ELISA for diagnosis of infections by viruses","volume":"53","author":"Yoshihara","year":"1995","journal-title":"Nihon Rinsho"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0166-0934(99)00167-6","article-title":"Flow cytometric detection of viruses","volume":"85","author":"Brussaard","year":"2000","journal-title":"J. Virol. Methods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1128\/JCM.01870-06","article-title":"Using a resequencing microarray as a multiple respiratory pathogen detection assay","volume":"45","author":"Lin","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/JCM.02202-06","article-title":"Comparison of the MChip to viral culture, reverse transcription-PCR, and the QuickVueinfluenzaA+B test for rapid diagnosis of influenza","volume":"45","author":"Mehlmann","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1002\/jmv.23272","article-title":"Broad respiratory virus detection in infants hospitalized for bronchiolitis by use of a multiplex RT-PCR DNA microarray system","volume":"84","author":"Huguenin","year":"2012","journal-title":"J. Med. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Rai, M., Reshetilov, A., Plekhanova, Y., and Ingle, A.P. (2021). SERS for Bacteria, Viruses, and Protein Biosensing. Macro, Micro and Nano-Biosensors. Potential Applications and Possible Limitations, Springer International Publisher.","DOI":"10.1007\/978-3-030-55490-3"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"037523","DOI":"10.1149\/2.0232003JES","article-title":"Review\u2014Chemical and biological sensors for viral detection","volume":"167","author":"Ozer","year":"2020","journal-title":"J. Electrochem. Soc."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Serra, P.A. (2011). Electrochemical Biosensors for Virus Detection. Biosensors for Health, Environment and Biosecurity, InTech. Chapter 14.","DOI":"10.5772\/928"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.virusres.2008.11.010","article-title":"Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation","volume":"140","author":"Qi","year":"2009","journal-title":"Virus Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rai, M., Reshetilov, A., Plekhanova, Y., and Ingle, A.P. (2021). Biosensors for virus detection. Macro, Micro and Nano-biosensors. Potential Applications and Possible Limitations, Springer International Publisher.","DOI":"10.1007\/978-3-030-55490-3"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Saylan, Y., Erdem, \u00d6., \u00dcnal, S., and Denizli, A. (2019). An alternative medical diagnosis method: Biosensors for virus detection. Biosensors, 9.","DOI":"10.3390\/bios9020065"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1134\/S0006350916010097","article-title":"Application of the method of electro-acoustical analysis for the detection of bacteriophages in a liquid phase","volume":"61","author":"Guliy","year":"2016","journal-title":"Biophysics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.talanta.2017.10.020","article-title":"Electro-acoustic sensor for the real-time identification of the bacteriophages","volume":"178","author":"Guliy","year":"2018","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1134\/S000635091703006X","article-title":"Use of mini-antibodies for detection of bacteriophages by the electroaucoustic analysis method","volume":"62","author":"Guliy","year":"2017","journal-title":"Biophysics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.snb.2018.04.063","article-title":"Sensor based on the slot acoustic wave for the non-contact analysis of the bacterial cells\u2013Antibody binding in the conducting suspensions","volume":"268","author":"Borodina","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00253-018-9476-5","article-title":"Prospects for the use of spherical gold nanoparticles in immunization","volume":"103","author":"Staroverov","year":"2019","journal-title":"Appl. Microbiol. Biot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4133","DOI":"10.1093\/nar\/19.15.4133","article-title":"Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (FAB) heavy and light chains","volume":"19","author":"Hoogenboom","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"ref_27","unstructured":"Sambrook, E.F., and Fritsch, T. (1989). Maniatis, Molecular Cloning: A Laborotory Manual, Cold Spring. Mavbov Lab. Press. [2nd ed.]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0022-1759(87)90187-6","article-title":"Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay","volume":"100","author":"Beatty","year":"1987","journal-title":"J. Immunol. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.1109\/TUFFC.2013.2867","article-title":"Acoustic Waves in a Structure Containing Two Piezoelectric Plates Separated by an Air (Vacuum) Gap","volume":"60","author":"Borodina","year":"2013","journal-title":"IEEE Trans. Ultrason. Ferroelectr. Freq. Control"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.jiph.2020.03.019","article-title":"Coronavirus disease 2019 (COVID-19): A literature review","volume":"13","author":"Harapan","year":"2020","journal-title":"J. Infect. Public Health"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1056\/NEJMp030078","article-title":"SARS-associated coronavirus","volume":"348","author":"Holmes","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1099\/0022-1317-67-7-1405","article-title":"Antigenic structure of transmissible gastroenteritis virus. II. Domains in the peplomer glycoprotein","volume":"67","author":"Delmas","year":"1986","journal-title":"J. Gen. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1128\/jvi.56.3.904-911.1985","article-title":"Proteolytic cleavage of the E2 glycoprotein of murine coronavirus by trypsin and separation of two different 90K cleavage fragments","volume":"56","author":"Sturman","year":"1985","journal-title":"J. Gen. Virol."},{"key":"ref_34","first-page":"197","article-title":"Biosensors for bacterial detection","volume":"2","author":"Chen","year":"2017","journal-title":"Int. J. Biosens. Bioelectron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ultras.2017.07.011","article-title":"The influence of viscous and conducting liquid on the characteristics of the slot acoustic wave","volume":"82","author":"Borodina","year":"2018","journal-title":"Ultrasonics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"121392","DOI":"10.1016\/j.talanta.2020.121392","article-title":"Diagnostic techniques for COVID-19 and new developments","volume":"220","author":"Sheikhzadeh","year":"2020","journal-title":"Talanta"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Nguyen, T., Bang, D.D., and Wolff, A. (2020). 2019 Novel coronavirus disease (COVID-19): Paving the road for rapid detection and point-ofcare diagnostics. Micromachines, 11.","DOI":"10.3390\/mi11030306"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5268","DOI":"10.1021\/acsnano.0c02439","article-title":"Dual-functional plasmonic photothermal biosensors for highly accurate severe acute respiratory syndrome coronavirus 2 detection","volume":"14","author":"Qiu","year":"2020","journal-title":"ACS Nano"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"112437","DOI":"10.1016\/j.bios.2020.112437","article-title":"Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19","volume":"166","author":"Zhu","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.jmoldx.2020.03.006","article-title":"Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)","volume":"22","author":"Park","year":"2020","journal-title":"J. Mol. Diagn."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1093\/clinchem\/hvaa102","article-title":"Rapid detection of COVID-19 coronavirus using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform","volume":"66","author":"Yu","year":"2020","journal-title":"Clin. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5135","DOI":"10.1021\/acsnano.0c02823","article-title":"Rapid COVID-19 detection causative virus (SARS-CoV-2) in human nasopharyngeal swab specimens using field-effect transistor-based biosensor","volume":"14","author":"Seo","year":"2020","journal-title":"ACS Nano"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"598103","DOI":"10.3389\/fpls.2020.598103","article-title":"Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers","volume":"11","author":"Poghossian","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.bios.2017.09.024","article-title":"Recent advances in nanowires-based field-effect transistors for biological sensor applications","volume":"100","author":"Ahmad","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR-Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_46","unstructured":"Zhang, X., Qi, Q., Jing, Q., Ao, S., Zhang, Z., Ding, M., Wu, M., Liu, K., Wang, W., and Ling, Y. (2020). Electrical probing of COVID-19 spike protein receptor binding domain via a graphene field-effect transistor. arXiv."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Mavrikou, S., Moschopoulou, G., Tsekouras, V., and Kintzios, S. (2020). Development of a portable, ultra-rapid and ultra-sensitive cell-based biosensor for the direct detection of the SARS-CoV-2 S1 spike protein antigen. Sensors, 20.","DOI":"10.3390\/s20113121"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"7617","DOI":"10.1021\/acsnano.0c03822","article-title":"Selective naked-eye detection of SARS-CoV-2 mediated by N gene targeted antisense oligonucleotide capped plasmonic nanoparticles","volume":"14","author":"Moitra","year":"2020","journal-title":"ACS Nano"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"125605","DOI":"10.1016\/j.optcom.2020.125605","article-title":"Progress in the use of an electro-optical sensor for virus detection","volume":"465","author":"Guliy","year":"2020","journal-title":"Opt. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7226","DOI":"10.1021\/acs.analchem.0c00784","article-title":"Rapid and sensitive detection of anti-SARS-CoV-2 IgG using lanthanide-doped nanoparticles-based lateral flow immunoassay","volume":"92","author":"Chen","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"112435","DOI":"10.1016\/j.bios.2020.112435","article-title":"Real-time diagnosis of reactive oxygen species (ROS) in fresh sputum by electrochemical tracing; correlation between COVID-19 and viral-induced ROS in lung\/respiratory epithelium during this pandemic","volume":"165","author":"Miripour","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Mahari, S., Roberts, A., Shahdeo, D., and Gandhi, S. (2020). eCovSens-Ultrasensitive novel in-house built printed circuit board based electrochemical device for rapid detection of nCovid-19 antigen, a spike protein domain 1 of SARS-CoV-2. bioRxiv.","DOI":"10.1101\/2020.04.24.059204"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3536","DOI":"10.1021\/ac035367b","article-title":"Piezoelectric Immunosensor for SARS-Associated Coronavirus in Sputum","volume":"76","author":"Zuo","year":"2004","journal-title":"Anal. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/j.bios.2007.12.016","article-title":"Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor","volume":"23","author":"Bisoffi","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.rbmret.2007.12.001","article-title":"Multipurpose Love acoustic wave immunosensor for bacteria, virus or proteins detection","volume":"29","author":"Moll","year":"2008","journal-title":"IRBM"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.bios.2006.06.030","article-title":"Quartz crystal microbalance immunosensors for environmental monitoring","volume":"22","author":"Kurosawa","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"12658","DOI":"10.3390\/s140712658","article-title":"Love-Wave Sensors Combined with Microfluidics for Fast Detection of Biological Warfare Agents","volume":"14","author":"Matatagui","year":"2014","journal-title":"Sensors"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/21\/5\/1822\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:33:41Z","timestamp":1760160821000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/21\/5\/1822"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,5]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["s21051822"],"URL":"https:\/\/doi.org\/10.3390\/s21051822","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,5]]}}}