{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T20:01:22Z","timestamp":1771531282320,"version":"3.50.1"},"reference-count":89,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T00:00:00Z","timestamp":1620864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>HLA-B*15:02 screening before administering carbamazepine is recommended to prevent life-threatening hypersensitivity. However, the unavailability of a point-of-care device impedes this screening process. Our research group previously developed a two-step HLA-B*15:02 detection technique utilizing loop-mediated isothermal amplification (LAMP) on the tube, which requires two-stage device development to translate into a portable platform. Here, we report a heater-integrated lab-on-a-chip device for the LAMP amplification, which can rapidly detect HLA-B alleles colorimetrically. A gold-patterned micro-sized heater was integrated into a 3D-printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA-B genotypes in the LAMP-chip parallel with the tube assay. The LAMP-on-chip results showed a complete match with the LAMP-on-tube assay, demonstrating the detection system\u2019s concurrence.<\/jats:p>","DOI":"10.3390\/s21103413","type":"journal-article","created":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T03:28:36Z","timestamp":1620962916000},"page":"3413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Heater Integrated Lab-on-a-Chip Device for Rapid HLA Alleles Amplification towards Prevention of Drug Hypersensitivity"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1295-8796","authenticated-orcid":false,"given":"Shah Mukim","family":"Uddin","sequence":"first","affiliation":[{"name":"Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7729-0240","authenticated-orcid":false,"given":"Abkar","family":"Sayad","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxiong","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia"},{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duc Hau","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efstratios","family":"Skafidas","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia"},{"name":"Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Melbourne, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Kwan","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia"},{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, VIC 3004, Australia"},{"name":"Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Melbourne, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1001\/archderm.1993.01680220104023","article-title":"Clinical classification of cases of toxic epidermal necrolysis, Stevens-Johnson syndrome, and erythema multiforme","volume":"129","author":"Rzany","year":"1993","journal-title":"Arch. 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