{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:36:53Z","timestamp":1782801413024,"version":"3.54.5"},"reference-count":28,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T00:00:00Z","timestamp":1621036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009448","name":"Universit\u00e0 degli Studi della Campania Luigi Vanvitelli","doi-asserted-by":"publisher","award":["VALERE"],"award-info":[{"award-number":["VALERE"]}],"id":[{"id":"10.13039\/501100009448","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 \u00b5L and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8\u201325.8 U\/L and a limit of detection (LOD) of ~0.5 U\/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of &gt;92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy.<\/jats:p>","DOI":"10.3390\/s21103443","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T02:31:34Z","timestamp":1621218694000},"page":"3443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9171-7856","authenticated-orcid":false,"given":"Laura","family":"Pasquardini","sequence":"first","affiliation":[{"name":"Indivenire srl, Via Alla Cascata 56\/C, 38123 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7769-0984","authenticated-orcid":false,"given":"Nunzio","family":"Cennamo","sequence":"additional","affiliation":[{"name":"Department of Engineering, University of Campania \u201cL. Vanvitelli\u201d, Via Roma 29, 81031 Aversa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Malleo","sequence":"additional","affiliation":[{"name":"Unit of Pancreatic Surgery, University of Verona Hospital Trust, Department of Surgery, Dentistry, Gynecology and Pediatrics (DSCOMI), University of Verona, P. le Scuro 10, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lia","family":"Vanzetti","sequence":"additional","affiliation":[{"name":"Fondazione Bruno Kessler-Sensors&amp;Devices e Micro Nano Facility, Via Sommarive 18, 38123 Povo-Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Zeni","sequence":"additional","affiliation":[{"name":"Department of Engineering, University of Campania \u201cL. Vanvitelli\u201d, Via Roma 29, 81031 Aversa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Bonamini","sequence":"additional","affiliation":[{"name":"Unit of Pancreatic Surgery, University of Verona Hospital Trust, Department of Surgery, Dentistry, Gynecology and Pediatrics (DSCOMI), University of Verona, P. le Scuro 10, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Salvia","sequence":"additional","affiliation":[{"name":"Unit of Pancreatic Surgery, University of Verona Hospital Trust, Department of Surgery, Dentistry, Gynecology and Pediatrics (DSCOMI), University of Verona, P. le Scuro 10, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Bassi","sequence":"additional","affiliation":[{"name":"Unit of Pancreatic Surgery, University of Verona Hospital Trust, Department of Surgery, Dentistry, Gynecology and Pediatrics (DSCOMI), University of Verona, P. le Scuro 10, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2542-8412","authenticated-orcid":false,"given":"Alessandra Maria","family":"Bossi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.surg.2005.05.001","article-title":"Postoperative pancreatic fistula: An international study group (ISGPF) definition","volume":"138","author":"Bassi","year":"2005","journal-title":"Surgery"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1097\/SLA.0b013e3180caa42f","article-title":"Amylase value in drains after pancreatic resection as predictive factor of postoperative pancreatic fistula: Results of a prospective study in 137 patients","volume":"246","author":"Molinari","year":"2007","journal-title":"Ann. 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